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A comprehensive review of fish protein hydrolysates targeting pet food formulat...

Ribeiro, Tânia Bragança; Maia, Margarida R. G.; Fonseca, António J. M.; Marques, Bianca; Caleja, Cristina; Rosa, Ana; Martins, Rui; Almeida, André

The fish industry generates significant amounts of fish by- and co-products (FBCPs) annually, projected to reach 160.8 million tonnes by 2030. This growth highlights the urgent need for sustainable FBCP management and an opportunity to improve the sector's environmental sustainability. Fish protein hydrolysates (FBCPHs) and bioactive peptides (BPs) derived from these FBCPs are gaining recognition in the pet foo...


Circular animal protein hydrolysates: a comparative approach of functional prop...

Monteiro, Marta; Rodrigues-dos-Santos, Luciano; Filipa-Silva, Andreia; Marques, Diana A.; Pintado, Manuela; Almeida, André; Valente, Luisa M. P.

The growing demand for nutraceuticals has driven interest in upcycling low-value proteins from processed animal by-products and insect larvae into functional protein hydrolysates. This study evaluated five such hydrolysates in comparison to a high-value commercial reference (CPSP90), assessing the proximate composition, amino acid profile, molecular weight distribution, antioxidant activity, and bacterial growt...


Short and Long-Term Trajectories of the Post COVID-19 Condition: Results From t...

Santoro, Andrea; Bai, Francesca; Greco, Maria Francesca; Rovito, Roberta; Sala, Matteo; Borghi, Lidia; Piscopo, Kyrie; Vegni, Elena

Background: Post COVID-19 condition (PCC) affects 10-40% of patients and is characterized by persisting symptoms at ≥ 4 weeks after SARS-CoV-2 infection. Symptoms can last 7 or even more months. How long PCC persists and any changes in its clinical phenotypes over time require further investigation. We investigated PCC trajectories and factors associated with PCC persistence. Material and methods: We included b...


Bromelain-catalyzed hydrolysis of fish and poultry by-products: a sustainable a...

Borges, Sandra; Ribas, Tânia C. F.; Castro, Maria Leonor; Campos, Débora; Mota, Maria João; Almeida, André; Pintado, Manuela

This study aimed to develop bioactive protein hydrolysates from fish and poultry by-products using bromelain, an enzyme obtained from pineapple by-products, with potential applications in food and biotechnology. The global rise in protein-rich food consumption has led to increased generation of animal by-products from the meat and fish industries. These by-products are rich in protein, making them suitable for ...


A comprehensive review of fish protein hydrolysates targeting pet food formulat...

Ribeiro, Tânia Bragança; Maia, Margarida R. G.; Fonseca, António J. M.; Marques, Bianca; Caleja, Cristina; Rosa, Ana; Martins, Rui; Almeida, André

The fish industry generates significant amounts of fish by- and co-products (FBCPs) annually, projected to reach 160.8 million tonnes by 2030. This growth highlights the urgent need for sustainable FBCP management and an opportunity to improve the sectors environmental sustainability. Fish protein hydrolysates (FBCPHs) and bioactive peptides (BPs) derived from these FBCPs are gaining recognition in the pet food...


A flow-based method as a more sustainable tool for total protein monitoring in ...

Teixeira, Raquel; Ribas, Tânia C. F.; Almeida, André; Pintado, Manuela; Rangel, António O. S. S.

The growing interest in protein hydrolysates highlights the need for fast and reliable analytical methods to assess their nutritional and functional properties. Total protein quantification is a key parameter for characterizing by-products, monitoring hydrolysis processes, and evaluating hydrolysates. In this work, a sustainable flow-based spectrophotometric method was developed and optimized using the Biuret m...


Sequential injection chromatographic system for the expeditious molecular weigh...

Teixeira, Raquel; Ribas, Tânia C. F.; Almeida, André; Pintado, Manuela; Rangel, António O. S. S.

Introduction and objective: According to European Union regulations, the use of additives in animal feed must be rigorously evaluated to ensure safety, efficacy, and quality. The guidelines highlight the importance of reliable and efficient analytical tools for characterizing bioactive compounds in feed. Currently, protein evaluation is predominantly performed using High-Performance Liquid Chromatography (HPLC)...


Sequential injection chromatographic system for the expeditious molecular weigh...

Teixeira, Raquel; Ribas, Tânia C. F.; Almeida, André; Pintado, Manuela; Rangel, António O. S. S.

According to the regulations of the European Union, the use of additives in animal nutrition must be rigorously assessed to ensure safety, efficacy, and quality. These guidelines emphasize the importance of reliable and efficient analytical tools for characterizing bioactive compounds in feed formulation [1]. Currently, the evaluation of proteins is predominantly performed using high-performance liquid chromato...


A flow-based method as a more sustainable tool for total protein monitoring in ...

Teixeira, Raquel; Ribas, Tânia C. F.; Almeida, André; Pintado, Manuela; Rangel, António O. S. S.

Introduction: This proposal aims to promote the circular economy, minimizing waste and enhancing environmental sustainability. In collaboration with several companies, the project uses by-products, subjecting them to hydrolysis processes to obtain hydrolysates. Objective: The main objective is to develop a flow spectrophotometric method to quantify the total soluble protein of these by-products, valuing them in...


Locally-sourced animal protein hydrolysates in high-plant-protein diets can pro...

Rodrigues-dos-Santos, Luciano; Basto, Ana; Monteiro, Marta; António, Carla; Rodrigues, Ana M.; Sá, Tiago; Velasco, Cristina; Martins, Rui; Rosa, Ana

Upcycling meat and fish by-products into bioactive protein hydrolysates promotes zero-waste practices within the circular bioeconomy and provides locally-sourced alternatives to replace fishmeal (FM) in aquafeeds. In this study, novel hydrolysates were developed from blue shark skin, fish by-products, and swine processed animal protein, aiming to reduce the high-quality FM inclusion in European seabass (Dicentr...


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