4 documents found, page 1 of 1
Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone rege...
Vasconcelos, DM; Gonçalves, RM; Almeida, CR; Pereira, IO; Oliveira, MI; Neves, N; Silva, AM; Ribeiro, AC; Cunha, C; Almeida, AR; Ribeiro, CC; Gil, AMThe hypothesis behind this work is that fibrinogen (Fg), classically considered a pro-inflammatory protein, can promote bone repair/regeneration. Injury and biomaterial implantation naturally lead to an inflammatory response, which should be under control, but not necessarily minimized. Herein, porous scaffolds entirely constituted of Fg (Fg-3D) were implanted in a femoral rat bone defect and investigated at tw...
Studying T Cells N-Glycosylation by Imaging Flow Cytometry
Dias, A; Almeida, CR; Reis, CA; Pinho, SSImaging flow cytometry is an emerging imaging technology that combines features of both conventional flow cytometry and fluorescence microscopy allowing quantification of the imaging parameters. The analysis of protein posttranslational modifications by glycosylation using imaging flow cytometry constitutes an important bioimaging tool in the glycobiology field. This technique allows quantification of the glyca...
Finding and tracing human MSC in 3D microenvironments with the photoconvertible...
Caires, HR; Gomez-Lazaro, M; Oliveira, CM; Gomes, D; Mateus, DD; Oliveira, C; Barrias, CC; Barbosa, MA; Almeida, CRMesenchymal Stem/Stromal Cells (MSC) are a promising cell type for cell-based therapies - from tissue regeneration to treatment of autoimmune diseases - due to their capacity to migrate to damaged tissues, to differentiate in different lineages and to their immunomodulatory and paracrine properties. Here, a simple and reliable imaging technique was developed to study MSC dynamical behavior in natural and bioeng...
Dysregulation of T cell receptor N-glycosylation: A molecular mechanism involve...
Dias, A; Dourado, J; Lago, P; Cabral, J; Marcos-Pinto, R; Salgueiro, P; Almeida, CR; Carvalho, S; Fonseca, S; Lima, M; Vilanova, M; Dinis-Ribeiro, MThe incidence of inflammatory bowel disease is increasing worldwide and the underlying molecular mechanisms are far from being fully elucidated. Herein, we evaluated the role of N-glycosylation dysregulation in T cells as a key mechanism in the ulcerative colitis (UC) pathogenesis. The evaluation of the branched N-glycosylation levelsandprofile of intestinalTcell receptor (TCR)wereassessedin colonic biopsies fr...