69 documents found, page 1 of 7
Assessing host-adaptation of new sequence types of livestock-associated methici...
Oliveira, Ricardo; Araújo, Daniela; Castro, Joana Isabel Reis; Nogueira, Teresa; Almeida, Gonçalo; Azevedo, Nuno F.; Almeida, CarinaThe importance of animals as reservoirs of human pathogens is widely recognised, with livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) being consistently reported. This study presents a phenotypic and genotypic characterization of five MRSA isolates from raw bovine milk in northern Portugal, using culturomics and whole genome sequencing (WGS), to assess their potential risk for human i...
Persistence of Listeria monocytogenes in food processing environments: challeng...
Domingues, Célia P. F.; Almeida, Gonçalo; Teixeira, Paula; Nogueira, TeresaListeriosis, caused by Listeria monocytogenes, remains one of the most serious foodborne illness in high-income countries, despite its rarity. This paper addresses the challenge of persistent L. monocytogenes in food processing environments, which leads to significant public health and economic impacts. We examine the persistence mechanisms of L. monocytogenes, focusing on natural selection due to disinfectant ...
Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relation...
Santos, Dércia; Pinto, Ana Rita; Barata, Rita; Fernandes, Conceição; Guedes, Hugo; Almeida, Gonçalo; Cabecinha, Edna; Monteiro, Sandra M.Coastal environments have been recognized as key reservoirs for antibiotic-resistant bacteria. The present study evaluated marine gastropods, Phorcus lineatus, as potential bioindicators to assess the spread of antibiotic-resistant bacteria. P. lineatus was sampled in four sites, with different anthropogenic pressures, along the northwest Portuguese coastal area. From these specimens, bacteria were isolated and...
Multidrug-Resistant Escherichia coli Accumulated by Freshwater Bivalves: An Und...
Martins, Joana; Gonçalves, Ana; Fernandes, Conceição; Cabecinha, Edna; Monteiro, Sandra Mariza; Guedes, Hugo; Almeida, Gonçalo; Garcia, JulianaAs bioindicators, freshwater bivalves are crucial for the assessment of the contamination impact on different levels of biological integration. Escherichia coli is used as a bioindicator of water fecal contamination, representing a critical global concern, especially with the rise of multidrugresistant (MDR) strains. Phylogenetic diversity, pathotypic characterization, and antibiotic resistance profiles of E. c...
Assessing the persister state in Listeria monocytogenes in response to gentamic...
Sousa, Mariana; Azevedo, Mónica; Magalhães, Rui; Almeida, Gonçalo; Teixeira, PaulaIntroduction: In the context of food safety, persister cells are a public health concern since these may contribute to the persistence and survival of bacteria in food processing environments. Listeria monocytogenes persisters are described as a subpopulation of growth-arrested cells that possess non-heritable, temporary tolerance to lethal doses of antibiotics (e.g., gentamicin) and can revert to an active sta...
High salinity and temperature contribute to Listeria monocytogenes persistence ...
Azevedo, Mónica; Sousa, Mariana; Magalhães, Rui; Almeida, Gonçalo; Teixeira, PaulaIntroduction: Foodborne listeriosis has a low case rate but high mortality, posing a public health concern. Listeria monocytogenes can contaminate food-processing and storage environments, compromising the food supply chain. L. monocytogenes adapts to stresses like nutrient deprivation, extreme temperatures, and high salinity. Understanding its persistence mechanisms, including the ability to enter a viable but...
Assessing the persister state to benzalkonium chloride in Listeria monocytogenes
Sousa, Mariana; Azevedo, Mónica; Magalhães, Rui; Almeida, Gonçalo; Teixeira, PaulaIntroduction: Listeria monocytogenes (Lm) persisters are a subpopulation of growth-arrested cells that possess non-heritable, temporary tolerance to lethal doses of antibiotics (e.g., gentamicin), which can be studied through biphasic killing curves – initial drop of susceptible cells followed by a plateau of persisters in response to continuous antibiotic exposure or to its increasing concentration. In the con...
Occurrence and diversity of Listeria monocytogenes in Portuguese dairy farms
Nunes, Bárbara; Barata, Ana Rita; Oliveira, Ricardo; Guedes, Hugo; Almeida, Carina Manuela Fernandes; Silva, Gabriela Jorge da; Nogueira, TeresaListeria monocytogenes is a pathogenic microorganism that causes listeriosis, an infection that usually occurs after consumption of contaminated food and is considered particularly dangerous due to its ability to grow and multiply under adverse conditions. In recent years, there has been an increase in the consumption of unprocessed products, such as raw milk and dairy products, by people of all ages, including...
Occurrence and diversity of Listeria monocytogenes in Portuguese dairy farms
Nunes, Bárbara; Barata, Ana Rita; Oliveira, Ricardo; Guedes, Hugo; Almeida, Carina; Jorge da Silva, Gabriela; Nogueira, Teresa; Saavedra, Maria JoséListeria monocytogenes is a pathogenic microorganism that causes listeriosis, an infection that usually occurs after consumption of contaminated food and is considered particularly dangerous due to its ability to grow and multiply under adverse conditions. In recent years, there has been an increase in the consumption of unprocessed products, such as raw milk and dairy products, by people of all ages, including...
Reimplante dentário intencional: revisão narrativa
Almeida, GonçaloO Reimplante Intencional é um procedimento que tem como objetivo dar solução a dentes que não aparentam ter solução que muitas vezes, com indicação para extração, permitindo a sua manutenção em boca. Apesar de ser considerado como um procedimento de último recurso, atualmente dá-se muito valor à preservação da dentição natural tornando o reimplante intencional especialmente relevante como uma das “ferramentas” ...