Coastal environments have been recognized as key reservoirs for antibiotic-resistant bacteria. The present study evaluated marine gastropods, Phorcus lineatus, as potential bioindicators to assess the spread of antibiotic-resistant bacteria. P. lineatus was sampled in four sites, with different anthropogenic pressures, along the northwest Portuguese coastal area. From these specimens, bacteria were isolated and...
O objetivo deste documento é informar a indústria alimentar sobre os riscos associados à contami- nação por L. monocytogenes. A presença desta bactéria em produtos acabados ou ambientes de pro- cessamento alimentar representa sérias ameaças à saúde do consumidor e pode levar a consequências económicas significativas. Este manual tem como objetivo alertar a indústria para os riscos recorrentes da contaminação e ...
Listeriosis, caused by Listeria monocytogenes, remains one of the most serious foodborne illness in high-income countries, despite its rarity. This paper addresses the challenge of persistent L. monocytogenes in food processing environments, which leads to significant public health and economic impacts. We examine the persistence mechanisms of L. monocytogenes, focusing on natural selection due to disinfectant ...
The importance of animals as reservoirs of human pathogens is widely recognised, with livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) being consistently reported. This study presents a phenotypic and genotypic characterization of five MRSA isolates from raw bovine milk in northern Portugal, using culturomics and whole genome sequencing (WGS), to assess their potential risk for human i...
The Kerlan-Jobe Orthopaedic Clinic Shoulder & Elbow Score (KJOC) is used to identify dysfunctions and estimate injury risk in overhead sports athletes. Although it has been validated in several countries, a European Portuguese version is currently unavailable. This study aimed to translate, culturally adapt and assess psychometric properties (validity and reliability) of the European Portuguese KJOC (KJOC-PT). ...
Listeria monocytogenes is a pathogenic microorganism that causes listeriosis, an infection that usually occurs after consumption of contaminated food and is considered particularly dangerous due to its ability to grow and multiply under adverse conditions. In recent years, there has been an increase in the consumption of unprocessed products, such as raw milk and dairy products, by people of all ages, including...
Introduction: Listeria monocytogenes (Lm) persisters are a subpopulation of growth-arrested cells that possess non-heritable, temporary tolerance to lethal doses of antibiotics (e.g., gentamicin), which can be studied through biphasic killing curves – initial drop of susceptible cells followed by a plateau of persisters in response to continuous antibiotic exposure or to its increasing concentration. In the con...
Introduction: In the context of food safety, persister cells are a public health concern since these may contribute to the persistence and survival of bacteria in food processing environments. Listeria monocytogenes persisters are described as a subpopulation of growth-arrested cells that possess non-heritable, temporary tolerance to lethal doses of antibiotics (e.g., gentamicin) and can revert to an active sta...
Introduction: Foodborne listeriosis has a low case rate but high mortality, posing a public health concern. Listeria monocytogenes can contaminate food-processing and storage environments, compromising the food supply chain. L. monocytogenes adapts to stresses like nutrient deprivation, extreme temperatures, and high salinity. Understanding its persistence mechanisms, including the ability to enter a viable but...