68 documents found, page 1 of 7

One-step purification of L-asparaginase from cell extracts using carbon xerogels

L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme with wide applications in the pharmaceutical sector and food processing industries. It is mainly used as a biotherapeutic for treating Acute Lymphoblastic Leukemia (ALL) and to reduce acrylamide formation in starchy compounds. Despite its relevance, current purification methods for microbial enzymes involve complex and expensive techniques. To overcome this drawb...

Supported ionic liquids to purify microbial L-Asparaginase

L-Asparaginase (ASNase) is a versatile enzyme that converts L-asparagine into ammonia and aspartic acid. This enzyme has applications in the food industry and health sector. However, high purity ASNase is required, resulting in high production costs. Therefore, in this work, two supported ionic liquids (SILs), specifically silica modified with dimethylbutylpropylammonium chloride ([Si][N3114]Cl) or triethylprop...

Supported ionic liquids for L-asparaginase downstream processing

L-asparaginase is an enzyme applied as a biopharmaceutical, as an acrylamide reduction agent and in the development of biosensors for the detection of L-asparagine (Nunes et al., 2020). L-asparaginase is widely distributed in nature, but microorganisms are the preferential source of production since they easily grow on inexpensive substrates (Lopes et al., 2017). For all the described applications, high levels ...

Purification of L-asparaginase through supported ionic liquid

L-asparaginase (ASNase) is an amidohydrolase enzyme applied as an anticancer agent in lymphoproliferative disorders treatment, as an acrylamide reduction agent in starch-rich foods cooked at high temperatures, and as a L-asparagine biosensor. ASNase is widely distributed in nature, e.g., microorganisms, plants and tissues of several animals. Nevertheless, most commercial ASNase are from recombinant microorganis...

Novel flow-through like mode platform for the purification of L-asparaginase

L-Asparaginase, an amidohydrolase enzyme applied in the pharmaceutical and food industries, is widely distributed in nature. Microorganisms are the preferential source of L-asparaginase production since they easily grow on inexpensive substrates. However, high levels of enzyme purity are required for all industrial applications, highlighting the need of developing cost-effective L-asparaginase purification proc...

Multi-walled carbon nanotubes as a platform for Immunoglobulin G attachment

Nanomaterials have been extensively used in different applications due to their peculiar characteristics and nanoscale dimensions. Among nanoparticles, carbon-based nanomaterials are becoming highly attractive for biomedical applications such as diagnosis, tissue engineering, drug delivery, and biosensing. The conjugation of carbon-based nanomaterials with antibodies combines the properties of these materials w...

A flow-through strategy using supported ionic liquids for L-asparaginase purifi...

Towards the sustainable extraction and purification of non-animal proteins from...

Advances towards the development of a sustainable economy must address the exploitation of bio-based products combined with the use of greener solvents and manufacturing processes that can preserve natural resources and the environment. To address the growing demand for proteins, their production must focus on non-animal sources, such as vegetal biomass and respective residues/waste, and on the use of sustainab...

Superior operational stability of immobilized l-asparaginase over surface-modif...

Made available in DSpace on 2022-04-28T19:46:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Fundação para a Ciência e a Tecnologia; l-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes the l-asparagine hydrolysis into l-aspartic acid and ammonia, being mainly applied in pharmaceutical and food industries. However, some d...

Enhanced Enzyme Reuse through the Bioconjugation of L-Asparaginase and Silica-B...

Made available in DSpace on 2022-04-28T19:50:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01; L-asparaginase (ASNase) is an amidohydrolase that can be used as a biopharmaceutical, as an agent for acrylamide reduction, and as an active molecule for L-asparagine detection. However, its free form displays some limitations, such as the enzyme’s single use and low stability. Hence, immobilization is ...