Objective: To assess the effectiveness of an educational hypermedia in the knowledge of Nursing academics on peripheral venipuncture. Method: Quasi-experimental study with pre and post-test design. Results: The mean number of right answers of the 73 individuals who participated in the study, in the pre-test, was 7.7 (DP = 1.05), and 8.3 in the post-test (DP = 0.74). The comparation of pre and post hypermedia me...