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Assessing process variable effects on laccase-mediated treatment for enhanced s...

Michelin, Michele; Gomes, Daniel Gonçalves; Freitas, Emanuelle N.; Alnoch, Robson C.; Salgado, José Carlos S.; Coelho, Eduardo João Louro

Lignocellulosic materials (LCM) have gained popularity as a substitute for petroleum, enabling the production of biofuels and various compounds. Eucalyptus bark residue (EBR), generated by pulp and paper mills, is a prime illustration of such materials. The conversion of holocellulose in these materials typically involves the application of enzymes. Nevertheless, these materials may still contain lignin, widely...


Immobilization and application of the recombinant xylanase GH10 of Malbranchea ...

Alnoch, Robson C.; Alves, Gabriela S.; Salgado, Jose C. S.; Andrades, Diandra de; Freitas, Emanuelle Neiverth de; Nogueira, Karoline M. V.; Vici, Ana C.

Xylooligosaccharides (XOS) are widely used in the food industry as prebiotic components. XOS with high purity are required for practical prebiotic function and other biological benefits, such as antioxidant and inflammatory properties. In this work, we immobilized the recombinant endo-1,4-β-xylanase of Malbranchea pulchella (MpXyn10) in various chemical supports and evaluated its potential to produce xylooligos...


Immobilization and stabilization of acetyl xylan esteraseof Aspergillus nidulans

Alves, Gabriela S.; Andrades, Diandra de; Segato, Fernando; Michelin, Michele; Buckeridge, Marcos S.; Alnoch, Robson C.

[Excerpt] Acetyl xylan esterase (EC 3.1.1.72) are enzymes that cleave the ester bonds of the O-acetyl groups of the xylan backbone to create new sites for subsequent hydrolysis of other enzymes, such as endoxylanases (BIELY et al., 2014). D-xylose is one of the main constituents of xylan, the main class of hemicellulose. To degrade this structure, it is necessary that enzymatic hydrolysis occurs synergistically...


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