19 documents found, page 1 of 2
Systematic SARS-CoV-2 S-gene sequencing in wastewater samples enables early lin...
Reis, Ana C.; Pinto, Daniela; Monteiro, Sílvia; Santos, Ricardo; Martins, João Vieira; Sousa, Alexandra; Páscoa, Rute; Lourinho, Rita; Cunha, Mónica V.As the COVID-19 pandemic reached its peak, many countries implemented genomic surveillance systems to track the evolution and transmission of SARS-CoV-2. Transition from the pandemic to the endemic phase prioritized alternative testing strategies to maintain effective epidemic surveillance at the population level, with less intensive sequencing efforts. One such promising approach was Wastewater-Based Surveilla...
Genomic epidemiology sheds light on the emergence and spread of Mycobacterium b...
Pereira, André C.; Reis, Ana C.; Cunha, Mónica V.Animal tuberculosis (TB) remains a serious concern for animal and human health. Mycobacterium bovis circulates in multi-host systems, dominated by the European 2 clonal complex (Eu2) in Iberia. In this work, we use genomic epidemiology to infer the emergence, spread, and spatiotemporal patterns of Eu2 in the official epidemiological risk area of animal TB in Portugal. Phylogenetic analysis of 144 M. bovis whole...
Empathy and Coping in Allied Health Sciences: Gender Patterns
Dores, Artemisa Rocha; Martins, Helena; Reis, Ana C.; Carvalho, Irene P.This study aimed to examine the patterns of associations between empathy and coping among undergraduate men and women studying at Allied Health Sciences. This cross-sectional study is part of a larger longitudinal study conducted in an Allied Health Sciences School. Participants were 183 undergraduate students from 12 training programs (e.g., Physiotherapy, Occupational Therapy, Speech Therapy).
Whole Genome Sequencing Refines Knowledge on the Population Structure of Mycoba...
Reis, Ana C.; Salvador, Liliana C. M.; Robbe-Austerman, Suelee; Tenreiro, Rogério; Botelho, Ana; Albuquerque, Teresa; Cunha, Mónica V.Classical molecular analyses of Mycobacterium bovis based on spoligotyping and Variable Number Tandem Repeat (MIRU-VNTR) brought the first insights into the epidemiology of animal tuberculosis (TB) in Portugal, showing high genotypic diversity of circulating strains that mostly cluster within the European 2 clonal complex. Previous surveillance provided valuable information on the prevalence and spatial occurre...
Genome-wide estimation of recombination, mutation and positive selection enligh...
Reis, Ana C.; Cunha, Mónica V.Genome sequencing has reinvigorated the infectious disease research feld, shedding light on disease epidemiology, pathogenesis, host–pathogen interactions and also evolutionary processes exerted upon pathogens. Mycobacterium tuberculosis complex (MTBC), enclosing M. bovis as one of its animal-adapted members causing tuberculosis (TB) in terrestrial mammals, is a paradigmatic model of bacterial evolution. As oth...
Systems epidemiology to devise new interventions for multi-host tuberculosis
Reis, Ana C.Animal tuberculosis (TB) is an infectious disease of livestock and wildlife mainly caused by Mycobacterium bovis and, in a minor extent, Mycobacterium caprae. In Portugal, animal TB is maintained in a multi-host system involving livestock and wild ungulates. The understanding of the processes driving transmission at this interface is key to inform control. In this work, M. caprae isolates (n=55) from Portugal w...
The open pan-genome architecture and virulence landscape of Mycobacterium bovis
Reis, Ana C.; Cunha, Mónica V.Animal tuberculosis (TB) is an emergent disease caused by Mycobacterium bovis, one of the animal-adapted ecotypes of the Mycobacterium tuberculosis complex (MTC). In this work, whole-genome comparative analyses of 70 M. bovis were performed to gain insights into the pan-genome architecture. The comparison across M. bovis predicted genome composition enabled clustering into the core- and accessory-genome compone...
Ecological drivers of Mycobacterium avium subsp. paratuberculosis detection in ...
Cunha, Mónica V.; Rosalino, L. M.; Leão, Célia; Bandeira, Victor; Fonseca, Carlos; Botelho, Ana; Reis, Ana C.Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of Johne's disease or paratuberculosis, a chronic infection affecting domestic ruminants worldwide. Despite sporadic reports of MAP occurrence in non-ruminants, information on the risk factors predisposing for infection is still scarce and evidence of transmission paths linking the livestock-wildlife-environment interfaces also remains l...
Long-term molecular surveillance provides clues on a cattle origin for Mycobact...
Reis, Ana C.; Tenreiro, Rogério; Albuquerque, Teresa; Botelho, Ana; Cunha, Mónica V.Animal tuberculosis (TB), caused by Mycobacterium bovis, is maintained in Portugal in a multi-host system, with cattle, red deer and wild boar, playing a central role. However, the ecological processes driving transmission are not understood. The main aim of this study was thus to contribute to the reconstruction of the spatiotemporal history of animal TB and to refine knowledge on M. bovis population structure...
The hard numbers of tuberculosis epidemiology in wildlife: A meta‐regression an...
Reis, Ana C.; Ramos, Beatriz; Pereira, André C.; Cunha, Mónica V.Tuberculosis (TB) is a widespread disease that crosses the human and animal health boundaries, with infection being reported in wildlife, from temperate and subtropical to arctic regions. Often, TB in wild species is closely associated with disease occurrence in livestock but the TB burden in wildlife remains poorly quantified on a global level. Through meta-regression and systematic review, this study aimed to...