Cry8Ka5 is a mutant protein from Bacillus thuringiensis (Bt) that has been proposed for developing trans genic plants due to promising activity against coleopterans, like Anthonomus grandis (the major pest of Brazilian cotton culture). Thus, an early food safety assessment of Cry8Ka5 protein could provide valu able information to support its use as a harmless biotechnological tool. This study aimed to evaluate ...