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Potential protective role of amphibian skin bacteria against water mold saprole...

Costa, Sara; Proença, Diogo Neves; Alves, Artur; Morais, Paula V.; Lopes, Isabel

Amphibian populations have experienced a severe decline over the past 40 years, driven primarily by environmental pollution, habitat destruction, climate change, and disease. This work reports, for the first time, saprolegniosis in Pelophylax perezi egg masses and saprolegniosis in amphibians in Portugal. After isolation and phylogenetic analysis, the pathogen was identified as Saprolegnia australis. Following ...


Microbial consortia selection for the development of an innovative nature-based...

Moreira, Irina S.; Novo, Beatriz; Monteiro, Pedro; Pinto, Glória; Castro, Paula M. L.; Alves, Artur; Tacão, Marta; Alves, Marta

Air pollutants, like benzene, toluene, and xylene (BTX), pose significant health and environmental risks being associated with 6.7 million premature deaths annually. Despite plants natural air purification capabilities in green infrastructures, high pollution levels in cities can hinder urban greening when tolerance levels are exceeded. Therefore, this study aimed to develop plant-beneficial microbial consortia...


How temperature modulates the expression of pathogenesis-related molecules of t...

Félix, Carina; Meneses, Rodrigo; Gonçalves, Micael F. M.; Duarte, Ana S.; Jorrín-Novo, Jesus V.; van de Peer, Yves; Deforce, Dieter

Lasiodiplodia hormozganensis, initially recognized as a fungal plant pathogen, is recognized now acknowledged as a potential threat to humans. However, our understanding of the pathogenesis mechanisms of Lasiodiplodia species remains limited, and the impact of temperature on its pathogenicity is unclear. This study aims to elucidate the effects of temperature on the biology of L. hormozganensis, focusing on the...


P8 - Marine fungi exhibit antimicrobial activity against human oral pathogens

Correia, Bruna L.; Devesas, Daniela; Noites, Rita; Gomes, Ana T. P. C.; Esteves, Ana Cristina; Alves, Artur; Duarte, Ana Sofia

The emergence of resistance to antibiotics and antimycotics has become a challenge in the treatment of infectious diseases, including infections of the oral cavity. Marine fungi are a source of novel biologically active compounds, namely in what concerns the development of antimicrobial and anticancer solutions. Our study aimed to test the antimicrobial activity and the cytotoxicity of the extracts of the two r...


Recent progress in marine mycological research in different countries, and pros...

Pang, Ka-Lai; Jones, E. B. Gareth; Abdel-Wahab, Mohamed A.; Adams, Sarah J.; Alves, Artur; Azevedo, Egídia; Bahkali, Ali H.; Barata, Margarida

Early research on marine fungi was mostly descriptive, with an emphasis on their diversity and taxonomy, especially of those collected at rocky shores on seaweeds and driftwood. Subsequently, further substrata (e.g. salt marsh grasses, marine animals, seagrasses, sea foam, seawater, sediment) and habitats (coral reefs, deep-sea, hydrothermal vents, mangroves, sandy beaches, salt marshes) were explored for marin...


Molecular techniques and target selection for the identification of Candida spp...

Magalhães, Joana; Correia, Maria José; Silva, Raquel M.; Esteves, Ana Cristina; Alves, Artur; Duarte, Ana Sofia

Candida species are the causative agent of oral candidiasis, with medical devices being platforms for yeast anchoring and tissue colonization. Identifying the infectious agent involved in candidiasis avoids an empirical prescription of antifungal drugs. The application of high-throughput technologies to the diagnosis of yeast pathogens has clear advantages in sensitivity, accuracy, and speed. Yet, conventional ...


Unveiling the secretome of the fungal plant pathogen neofusicoccum parvum induc...

Pour, Forough Nazar; Pedrosa, Bruna; Oliveira, Micaela; Fidalgo, Cátia; Devreese, Bart; Driessche, Gonzalez Van; Félix, Carina; Rosa, Nuno; Alves, Artur

Neofusicoccum parvum is a fungal plant pathogen of a wide range of hosts but knowledge about the virulence factors of N. parvum and host–pathogen interactions is rather limited. The molecules involved in the interaction between N. parvum and Eucalyptus are mostly unknown, so we used a multi-omics approach to understand pathogen–host interactions. We present the first comprehensive characterization of the in vit...


Effect of the Combined Treatments with LC2017 and Trichoderma atroviride Strain...

Reis, Pedro; Mondello, Vincenzo; Diniz, Inês; Alves, Artur; Rego, Cecília; Fontaine, Florence

Grapevine trunk diseases constitute one of the major problems for viticulture worldwide, with Botryosphaeria dieback considered as one of the most important of these diseases. In this work, we aimed to (i) evaluate the effect of the combination of two products, Esquive® (a Trichoderma-based product) and LC2017 (a low-copper-based product), in the control of Lasiodiplodia theobromae, by evaluating the internal l...


Unveiling biological activities of marine fungi: the effect of sea salt

Gonçalves, Micael F. M.; Paço, Ana; Escada, Luís F.; Albuquerque, Manuela S. F.; Pinto, Carlos A.; Saraiva, Jorge A.; Duarte, Ana Sofia

There is an urgent need for new substances to overcome current challenges in the health sciences. Marine fungi are known producers of numerous compounds, but the manipulation of growth conditions for optimal compound production can be laborious and time-consuming. In Portugal, despite its very long coastline, there are only a few studies on marine fungi. From a collection of Portuguese marine fungi, we screened...


Combining an HA + CU (II) site-targeted copper-based product with a pruning wou...

Reis, Pedro; Gaspar, Ana; Alves, Artur; Fontaine, Florence; Rego, Cecília

The genus Lasiodiplodia has been reported from several grape growing regions and is considered as one of the fastest wood colonizers, causing Botryosphaeria dieback. The aim of this study was to (i) evaluate the efficacy of Esquive®, a biocontrol agent, on vineyard pruning wound protection, applied single or, in a combined protection strategy with a new site-targeted copper-based treatment (LC2017), and (ii) co...


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