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Conductive materials change the composition and activity of butyrate-degrading ...

Braga, Cátia Sofia Neves; Costa, João Carlos Sequeira; Duarte, Maria Salomé; Martins, Gilberto; Pereira, Inês A. C.; Pereira, Luciana; Alves, M. M.

Conductive materials (CM) can improve methane production (MP) efficiency in many methanogenic systems. However, several types of CM exist, and there are uncertainties regarding whether they all improve MP efficiency to the same extent and modulate microbial communities in a similar way. To investigate that, different microbial enrichments with and without activated carbon (AC), magnetite (Mag), and zeolites (Ze...


Effect of trace metal bioavailability on the anaerobic digestion of purified te...

Pinheiro, Aline Martins; Tavares, T.; Alves, M. M.; Duarte, Maria Salomé Lira

The production of purified terephthalic acid (PTA), a precursor for the plastic industry, employs metalscobalt (Co) and manganese (Mn)as catalysts, generating wastewater (PTA WW) that contains metals and organic compounds. Co and Mn are essential to microbial necessities during anaerobic digestion (AD), but they can potentially inhibit this process when present in high concentrations. This study investigated th...


Micro-aeration as a pre-treatment to decrease LCFA toxicity in anaerobic biorea...

Duarte, Maria Salomé Lira; Costa, Luís; Magalhães, Carla Isabel Pereira; Pereira, M. A.; Stams, Alfons. J. M; Alves, M. M.; Cavaleiro, Ana Júlia Viana

Introduction: Micro-aeration in anaerobic digestion (AD) has shown process improvement, namely in the degradation of long-chain fatty acid (LCFA). Under micro-aeration, oleate, a toxic LCFA, can be faster converted to methane [1,2]. This is related to the presence of facultative anaerobic bacteria (FAB) which seem to be linked to oleate-to-palmitate conversion, although their role in this transformation is not ...


Monitoring the stability of aerobic granular sludge under increasing fractions ...

Silva, Sérgio Alves; Duarte, Maria Salomé Lira; Amaral, A. Luís; Ferreira, Eugénio C.; Alves, M. M.; Mesquita, D. P.

This work investigates the effects of increasing fraction of slowly biodegradable chemical oxygen demand (sbCOD) on the morphology, stability, and performance of aerobic granular sludge (AGS) used for wastewater treatment. A sequencing batch reactor (SBR) was supplied with synthetic wastewater containing acetate as readily biodegradable COD (rbCOD) and increasing concentrations of oleate as slowly biodegradable...


Microbial activity of the inoculum determines the impact of activated carbon, m...

Braga, Cátia Sofia Neves; Martins, Gilberto; Duarte, Maria Salomé Lira; Soares, O. Salomé G. P.; Pereira, M. Fernando R.; Pereira, Inês A. C.

The conversion of organic matter to methane through anaerobic digestion (AD) process can be enhanced by different materials. However, literature reports show inconsistent results on the effect of materials in different AD systems. In this study, we evaluated the influence of the inoculum's activity on methane production (MP) efficiency in the presence of different materials (activated carbon (AC), magnetite (Ma...


MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

Dueholm, Morten Kam Dahl; Andersen, Kasper Skytte; Petersen, Anne-Kirstine C.; Rudkjøbing, Vibeke; Alves, M. M.; Bajón-Fernández, Yadira

Anaerobic digestion represents a key biotechnology for the transformation of organic waste into renewable energy (biogas) and relies on complex microbial communities that work in concert to degrade the complex substrates into methane and carbon dioxide. Here, we sequenced more than half a million high-quality, full-length 16S rRNA gene sequences from 285 full-scale anaerobic digesters (ADs) across the world to ...


Micro-aeration to improve oleate degradation in bioreactors

Magalhães, Carla Isabel Pereira; Duarte, Maria Salomé Lira; Pereira, M. A.; Stams, Alfons Johannes Maria; Cavaleiro, Ana Júlia Viana; Alves, M. M.

Oleate (OL) is a long-chain fatty acid (LCFA) abundant in lipid-rich wastewater, and it is toxic to anaerobic microbial communities. Previous works showed that OL was faster converted to methane when in the presence of small oxygen concentrations, entering in bioreactors without control. In this study, controlled micro-aeration was applied, triggered by redox potential (ORP), to drive OL biodegradation to metha...


MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

Dueholm, Morten Kam Dahl; Andersen, Kasper Skytte; Korntved, Anne-Kirstine C.; Rudkjøbing, Vibeke; Alves, M. M.; Bajón-Fernández, Yadira

Anaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing of 285 full-scale anaerobic digesters (ADs) to expand our knowledge about diversity and function of the bacteria and archaea in ADs worldwide. The sequences are processed into full-length 16S rRNA amplicon sequence variants (FL-ASVs) and...


Non-conductive silicon-containing materials improve methane production by pure ...

Braga, Cátia Sofia Macedo; Martins, Gilberto; Soares, O. Salomé G. P.; Pereira, M. Fernando R.; Pereira, Inês A. C.; Pereira, Luciana; Alves, M. M.

Conductive materials (CM) enhance methanogenesis, but there is no clear correlation between conductivity and faster methane production (MP) rates. We investigated if MP by pure cultures of methanogens (Methanobacterium formicicum, Methanospirillum hungatei, Methanothrix harundinacea and Methanosarcina barkeri) is affected by CM (activated carbon (AC), magnetite), and other sustainable alternatives (sand and gla...


MOSCA 2.0: a bioinformatics framework for metagenomics, metatranscriptomics and...

Costa, João Carlos Sequeira; Pereira, Vítor; Alves, M. M.; Pereira, M. A.; Rocha, Miguel; Salvador, Andreia Filipa Ferreira

The analysis of meta-omics data requires the utilization of several bioinformatics tools and proficiency in informatics. The integration of multiple meta-omics data is even more challenging, and the outputs of existing bioinformatics solutions are not always easy to interpret. Here, we present a meta-omics bioinformatics pipeline, Meta-Omics Software for Community Analysis (MOSCA), which aims to overcome these ...


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