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Adaptative biological response of aerobic granular sludge to events of single o...

Alves, Marta; Henriques, Isabel; Castro, Paula M. L.; Amorim, Catarina L.

Wastewater comprises various stressors and their individual and combined immediate effects on aerobic granular sludge (AGS) are still underexplored. In this study, the AGS was exposed for 24 hours to wastewater with varying salt concentrations (up to 30 g NaCl L⁻¹) alongside pharmaceuticals (diclofenac, DCF or carbamazepine, CBZ). Differences in extracellular polymeric substances (EPS) production and compositio...


Structural and functional shifts in the microbial community of a heavy metal-co...

Silva, Isabel; Alves, Marta; Malheiro, Catarina; Silva, Ana Rita R.; Loureiro, Susana; Henriques, Isabel; González-Alcaraz, M. Nazaret

The interplay between metal contamination and climate change may exacerbate the negative impact on the soil microbiome and, consequently, on soil health and ecosystem services. We assessed the response of the microbial community of a heavy metal-contaminated soil when exposed to short-term (48 h) variations in air temperature, soil humidity or ultraviolet (UV) radiation in the absence and presence of Enchytraeu...


Bioremediation of coastal aquaculture effluents spiked with florfenicol using m...

Oliveira, Ana S.; Alves, Marta; Leitão, Frederico; Tacão, Marta; Henriques, Isabel; Castro, Paula M. L.; Amorim, Catarina L.

Aquaculture is a crucial industry in the agri-food sector, but it is linked to serious environmental problems. There is a need for efficient treatment systems that allow water recirculation to mitigate pollution and water scarcity. This work aimed to evaluate the self-granulation process of a microalgae-based consortium and its capacity to bioremediate coastal aquaculture streams that sporadically contain the a...


Bioremediation of coastal aquaculture effluents spiked with florfenicol using m...

Oliveira, Ana S.; Alves, Marta; Leitão, Frederico; Tacão, Marta; Henriques, Isabel; Castro, Paula M. L.; Amorim, Catarina L.

Aquaculture is a crucial industry in the agri-food sector, but it is linked to serious environmental problems. There is a need for efficient treatment systems that allow water recirculation to mitigate pollution and water scarcity. This work aimed to evaluate the self-granulation process of a microalgae-based consortium and its capacity to bioremediate coastal aquaculture streams that sporadically contain the a...


Microplastics accumulate priority antibiotic-resistant pathogens: evidence from...

Silva, Isabel; Rodrigues, Elsa T.; Tacão, Marta; Henriques, Isabel

Microplastics (MPs) might accumulate and transport antibiotic-resistant bacteria (ARB) in aquatic systems. We determined the abundance and diversity of culturable ciprofloxacin- and cefotaxime-resistant bacteria in biofilms covering MPs placed in river water, and characterized priority pathogens from these biofilms. Our results showed that the abundance of ARB colonizing MPs tends to be higher compared to sand ...


Third generation cephalosporin-resistant Klebsiella pneumoniae thriving in pati...

Rocha, Jaqueline; Ferreira, Catarina; Mil-Homens, Dalila; Busquets, Antonio; Fialho, Arsénio M; Henriques, Isabel; Gomila, Margarita; Manaia, Célia M.

Klebsiella pneumoniae are ubiquitous bacteria and recognized multidrug-resistant opportunistic pathogens that can be released into the environment, mainly through sewage, where they can survive even after wastewater treatment. A major question is if once released into wastewater, the selection of lineages missing clinically-relevant traits may occur. Wastewater (n = 25) and clinical (n = 34) 3rd generation ceph...


Microbial Associations of Abyssal Gorgonians and Anemones (>4,000 m Depth) at t...

Quintanilla, Elena; Rodrigues, Clara F; Henriques, Isabel; Hilário, Ana

Deep coral-dominated communities play paramount roles in benthic environments by increasing their complexity and biodiversity. Coral-associated microbes are crucial to maintain fitness and homeostasis at the holobiont level. However, deep-sea coral biology and their associated microbiomes remain largely understudied, and less from remote and abyssal environments such as those in the Clarion-Clipperton Fracture ...


Short-Term Responses of Soil Microbial Communities to Changes in Air Temperatur...

Silva, Isabel; Alves, Marta; Malheiro, Catarina; Silva, Ana Rita R; Loureiro, Susana; Henriques, Isabel; González-Alcaraz, M Nazaret

We analyzed the effects on a soil microbial community of short-term alterations in air temperature, soil moisture and ultraviolet radiation and assessed the role of invertebrates (species Enchytraeus crypticus) in modulating the community's response to these factors. The reference soil, Lufa 2.2, was incubated for 48 h, with and without invertebrates, under the following conditions: standard (20 °C + 50% water ...


KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds

Teixeira, Pedro; Pinto, Nuno; Henriques, Isabel; Tacão, Marta

Carbapenems are antibiotics of pivotal importance in human medicine, the efficacy of which is threatened by the increasing prevalence of carbapenem-resistant Enterobacterales (CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban ponds over a one-year period and retrieved 23 isolates. These were submitted to BOX-PCR, PFGE, 16S r...


Food Ingredients Derived from Lemongrass Byproduct Hydrodistillation: Essential...

Rodrigues, Luís; Coelho, Elisabete; Madeira, Renata; Teixeira, Pedro; Henriques, Isabel; Coimbra, Manuel A.

Essential oil (EO), hydrolate, and nondistilled aqueous phase (decoction) obtained from the hydrodistillation of lemongrass byproducts were studied in terms of their potential as food ingredients under a circular economy. The EO (0.21%, dry weight basis) was composed mainly of monoterpenoids (61%), the majority being citral (1.09 g/kg). The minimal inhibitory concentrations (MIC) of lemongrass EO against Escher...


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