43 documents found, page 1 of 5
Long-term exposure to supraphysiological levels of testosterone impacts rat sub...
Valente-Santos, J; Vitorino, R; Sousa-Mendes, C; Oliveira, Paula; Colaço, B; Faustino-Rocha, Ana; Neuparth, MJ; Leite-Moreira, A; Duarte, JAThe salivary glands play a central role in the secretion of saliva, whose composition and volume affect oral and overall health. A lesser-explored dimension encompasses the possible changes in salivary gland proteomes in response to fluctuations in sex hormone levels. This study aimed to examine the effects of chronic exposure to testosterone on salivary gland remodeling, particularly focusing on proteomic adap...
Tracking Prostate Carcinogenesis over Time through Urine Proteome Profiling in ...
Moreira-Pais, Alexandra; Nogueira-Ferreira, Rita; Reis, Stephanie; Aveiro, Susana; Barros, António; Melo, Tânia; Matos, Bárbara; Duarte, José AlbertoProstate cancer (PCa) is one of the most lethal diseases in men, which justifies the search for new diagnostic tools. The aim of the present study was to gain new insights into the progression of prostate carcinogenesis by analyzing the urine proteome. To this end, urine from healthy animals and animals with prostate adenocarcinoma was analyzed at two time points: 27 and 54 weeks. After 54 weeks, the incidence ...
Tracking prostate carcinogenesis over time through urine proteome profiling in ...
Moreira-Pais, Alexandra; Nogueira-Ferreira, Rita; Reis, Stephanie; Aveiro, Susana; Barros, António; Melo, Tânia; Matos, Bárbara; Duarte, José AlbertoProstate cancer (PCa) is one of the most lethal diseases in men, which justifies the search for new diagnostic tools. The aim of the present study was to gain new insights into the progression of prostate carcinogenesis by analyzing the urine proteome. To this end, urine from healthy animals and animals with prostate adenocarcinoma was analyzed at two time points: 27 and 54 weeks. After 54 weeks, the incidence ...
Mining the Biomarker Potential of the Urine Peptidome: From Amino Acids Propert...
Trindade, Fábio; Barros, António Sousa; Silva, Jéssica; Vlahou, Antonia; Falcão-Pires, Inês; Guedes, Sofia; Vitorino, Carla; Ferreira, RitaNative biofluid peptides offer important information about diseases, holding promise as biomarkers. Particularly, the non-invasive nature of urine sampling, and its high peptide concentration, make urine peptidomics a useful strategy to study the pathogenesis of renal conditions. Moreover, the high number of detectable peptides as well as their specificity set the ground for the expansion of urine peptidomics t...
Taste sensitivity and lifestyle are associated with food preferences and BMI in...
Rodrigues, Lénia; Silvério, Rosa; Costa, Ana R.; Antunes, Célia; Pomar, Clarinda; Infante, Paulo; Conceição, Cristina; Amado, Francisco; Lamy, ElsaOral food perception together with lifestyle may affect food preferences and choices, influencing weight gain and obesity development. The present study was designed to evaluate the association of biological (taste sensitivity) and lifestyle variables with children food preferences, assessing whether all these variables contribute to explain BMI percentile. After anthropometric evaluation, 387 children were cla...
Association between estrogen receptors and GATA3 in bladder cancer: a systemati...
Bernardo, Carina; Monteiro, Fátima L.; Direito, Inês; Amado, Francisco; Afreixo, Vera; Santos, Lúcio L.; Helguero, Luisa A.Background: Estrogen receptors alpha (ERα) and beta (ERβ) and the cooperating protein GATA-binding factor 3 (GATA3) have been implicated in bladder carcinogenesis and tumour progression. GATA3 and ER have been functionally linked in the establishment of luminal fate in breast tissue, but to date their relationship in bladder cancer has not been established. This information will be useful to advance diagnostic ...
Protein Aggregation Patterns Inform about Breast Cancer Response to Antiestroge...
Direito, Inês; Monteiro, Liliana; Melo, Tânia; Figueira, Daniela; Lobo, João; Enes, Vera; Moura, Gabriela; Henrique, Rui; Santos, Manuel A. S.The protein quality control network, including autophagy, the proteasome and the unfolded protein response (UPR), is triggered by stress and is overactive in acquired antiestrogen therapy resistance. We show for the first time that the aggresome load correlates with apoptosis and is increased in antiestrogen-sensitive cells compared to endocrine-resistant variants. LC-MS/MS analysis of the aggregated proteins o...
Comparison of salivary proteome of children with different sensitivities for bi...
Rodrigues, Lénia; Espanca, Rosa; Costa, Ana; Antunes, Célia; Pomar, Clarinda; Capela e Silva, Fernando; Pinheiro, Cristina; Domingues, PedroBackground/objectives: Oral sensorial perception is a key aspect in food choices and knowing the mechanisms modulating such perception is of major importance in the context of child obesity, which is reaching high rates in Mediterranean countries. Salivary proteome has been linked to taste sensitivity in adults. The aim of this study was to search for differences in salivary proteomes of children with different...
Estrogen receptors in urogenital schistosomiasis and bladder cancer: Estrogen r...
Bernardo, Carina; Santos, Júlio; Costa, Céu; Tavares, Ana; Amaro, Teresina; Marques, Igor; Gouveia, Maria João; Félix, Vítor; Afreixo, VeraEstrogen-like metabolites have been identified in S. haematobium, the helminth parasite that causes urogenital schistosomiasis (UGS) and in patients´ blood and urine during UGS. Estrogen receptor (ER) activation is enriched in the luminal molecular subtype bladder cancer (BlaCa). To date, the significance of ER to these diseases remains elusive. We evaluated ERα and ERβ expression in UGS-related BlaCa (n = 27),...
De novo sequencing of proteins by mass spectrometry
Vitorino, Rui; Guedes, Sofia; Trindade, Fabio; Correia, Inês; Moura, Gabriela; Carvalho, Paulo; Santos, Manuel; Amado, FranciscoIntroduction Proteins are crucial for every cellular activity and unraveling their sequence and structure is a crucial step to fully understand their biology. Early methods of protein sequencing were mainly based on the use of enzymatic or chemical degradation of peptide chains. With the completion of the human genome project and with the expansion of the information available for each protein, various database...