32 documents found, page 1 of 4
Microglial Rac1 is essential for experience-dependent brain plasticity and cogn...
Socodato, Renato; Almeida, Tiago O.; Portugal, Camila C.; Santos, Evelyn C.S.; Tedim-Moreira, Joana; Ferreira, João Galvão; Canedo, TeresaMicroglia, the largest population of brain immune cells, continuously interact with synapses to maintain brain homeostasis. In this study, we use conditional cell-specific gene targeting in mice with multi-omics approaches and demonstrate that the RhoGTPase Rac1 is an essential requirement for microglia to sense and interpret the brain microenvironment. This is crucial for microglia-synapse crosstalk that drive...
Normative mice retinal thickness: 16-month longitudinal characterization of wil...
Batista, Ana; Guimarães, Pedro; Martins, João; Moreira, Paula I.; Ambrósio, António F.; Castelo-Branco, Miguel; Serranho, Pedro; Bernardes, RuiAnimal models of disease are paramount to understand retinal development, the pathophysiology of eye diseases, and to study neurodegeneration using optical coherence tomography (OCT) data. In this study, we present a comprehensive normative database of retinal thickness in C57BL6/129S mice using spectral-domain OCT data. The database covers a longitudinal period of 16 months, from 1 to 16 months of age, and pro...
Retinal imaging in animal models: searching for biomarkers of neurodegeneration
Batista, Ana; Guimarães, Pedro; Serranho, Pedro; Nunes, Ana; Martins, João; Moreira, Paula I.; Ambrósio, António F.; Morgado, MiguelThere is a pressing need for novel diagnostic and progression biomarkers of neurodegeneration. However, the inability to determine disease duration and stage in patients with Alzheimer’s disease (AD) hinders their discovery. Because animal models of disease allow us to circumvent some of these limitations, they have proven to be of paramount importance in clinical research. Due to the clear optics of the eye, t...
Longitudinal normative OCT retinal thickness data for wild-type mice, and chara...
Ferreira, Hugo; Martins, João; Moreira, Paula I.; Ambrósio, António F.; Castelo-Branco, Miguel; Serranho, Pedro; Bernardes, RuiMice are widely used as models for many diseases, including eye and neurodegenerative diseases. However, there is a lack of normative data for retinal thickness over time, especially at young ages. In this work, we present a normative thickness database from one to four-months-old, for nine layers/layer-aggregates, including the total retinal thickness, obtained from the segmentation of spectral-domain optical ...
The Benefits of Flavonoids in Diabetic Retinopathy
Matos, Ana L; Bruno, Diogo F; Ambrósio, António F.; Santos, Paulo FDiabetic retinopathy (DR), one of the most common complications of diabetes, is the leading cause of legal blindness among adults of working age in developed countries. After 20 years of diabetes, almost all patients suffering from type I diabetes mellitus and about 60% of type II diabetics have DR. Several studies have tried to identify drugs and therapies to treat DR though little attention has been given to ...
Microglia Dysfunction Caused by the Loss of Rhoa Disrupts Neuronal Physiology a...
Socodato, Renato; Portugal, Camila C; Canedo, Teresa; Rodrigues, Artur Filipe; Almeida, Tiago O; Henriques, Joana F; Vaz, Sandra H; Magalhães, JoãoNervous tissue homeostasis requires the regulation of microglia activity. Using conditional gene targeting in mice, we demonstrate that genetic ablation of the small GTPase Rhoa in adult microglia is sufficient to trigger spontaneous microglia activation, producing a neurological phenotype (including synapse and neuron loss, impairment of long-term potentiation [LTP], formation of β-amyloid plaques, and memory ...
Microglial Activation in the Retina of a Triple-Transgenic Alzheimer's Disease ...
Salobrar-García, Elena; Rodrigues-Neves, Ana C.; Ramírez, Ana I.; de Hoz, Rosa; Fernández-Albarral, José A.; López-Cuenca, Inés; Ramírez, José M.Alzheimer's disease (AD) is the most common type of dementia in the world. The main biomarkers associated with AD are protein amyloid-β (Aβ) plaques and protein tau neurofibrillary tangles, which are responsible for brain neuroinflammation mediated by microglial cells. Increasing evidence has shown that the retina can also be affected in AD, presenting some molecular and cellular changes in the brain, such as m...
Microglia dysfunction caused by the loss of rhoa disrupts neuronal physiology a...
Socodato, Renato; Portugal, Camila C.; Canedo, Teresa; Rodrigues, Artur; Almeida, Tiago O.; Henriques, Joana F.; Vaz, Sandra H.; Magalhães, JoãoNervous tissue homeostasis requires the regulation of microglia activity. Using conditional gene targeting in mice, we demonstrate that genetic ablation of the small GTPase Rhoa in adult microglia is sufficient to trigger spontaneous microglia activation, producing a neurological phenotype (including synapse and neuron loss, impairment of long-term potentiation [LTP], formation of β-amyloid plaques, and memory ...
Microglia dysfunction caused by the loss of Rhoa disrupts neuronal physiology a...
Socodato, Renato; Portugal, Camila C.; Canedo, Teresa; Rodrigues, Artur; Almeida, Tiago O.; Henriques, Joana F.; Vaz, Sandra H.; Magalhães, JoãoNervous tissue homeostasis requires the regulation of microglia activity. Using conditional gene targeting in mice, we demonstrate that genetic ablation of the small GTPase Rhoa in adult microglia is sufficient to trigger spontaneous microglia activation, producing a neurological phenotype (including synapse and neuron loss, impairment of long-term potentiation [LTP], formation of β-amyloid plaques, and memory ...
Sexual dimorphism of the adult human retina assessed by optical coherence tomog...
Nunes, Ana; Serranho, Pedro; Quental, Hugo; Ambrósio, António F.; Castelo-Branco, Miguel; Bernardes, RuiSexual dimorphism in the human visual system is a well-established phenomenon, and recent research has unveiled possible connections between gonadal hormones and the retina status. In the literature, the findings are quite diverse and inconclusive results have been reported as well. In the study herein, texture analysis was applied to computed optical coherence tomography (OCT) fundus images to identify differe...