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Meat processing wastewater valorised through microalgae production

Sousa, Ana S. S.; Oliveira, Ana T.; Castro, Paula M. L.; Amorim, Catarina L.

The treatment of meat processing wastewater (MPWW) is crucial to mitigating its environmental impact but remains challenging due to its high organic content and contents of nitrogen and solids. The novelty of this work lies in evaluating the treatment and valorization of real MPWW with variable composition, as well as the process scalability, in a fed-batch raceway reactor while exploring the synergistic microb...


Unveiling the agronomic potential of extracellular polymeric substances recover...

Amorim, Catarina L.; Pereira, Sofia I.A.; Sousa, Ana S. S.; Wilfert, Philipp; Loosdrecht, Mark van; Martins, António; Castro, Paula M. L.

Background: Extracellular polymeric substances (EPS) recovered from waste aerobic granular sludge (AGS) generated during wastewater treatment represent a promising route for resource recovery. As valuable biopolymers, EPS have garnered special attention across multiple sectors, particularly agriculture, although their benefits in this field remain understudied. This study assessed the agronomic potential of EPS...


Shifts in aerobic granular sludge bacteriome driven by seawater intrusion in wa...

Miranda, Catarina; Castro, Paula M. L.; Amorim, Catarina L.

High salinity levels in wastewater treatment plants (WWTPs) can be attributed to multiple sources, including seawater intrusion in wastewater streams. In coastal WWTPs, this phenomenon is becoming more frequent, causing transient salinity shocks on the microbial populations involved in the treatment process. Aerobic granular sludge (AGS) has emerged as a revolutionary technology that has been adopted worldwide ...


Enhancing photosynthetic efficiency and nutrient uptake in Maize (Zea mays L.) ...

Amorim, Catarina L.; Pereira, Sofia I. A.; Sousa, Ana S. S.; Wilfert, Philipp; Loosdrecht, Mark van; Martins, António; Castro, Paula M. L.

Extracellular polymeric substances (EPSs) are biopolymers that can be recovered from waste aerobic granular sludge (AGS) generated in wastewater treatment plants (WWTPs). These biopolymers represent a valuable resource with attractive attributes, making them suitable for applications across different sectors. In agriculture, EPSs have attracted growing interest, although their beneficial effects are still under...


Microbiome adaptation of aerobic granular sludge allows process resilience duri...

Miranda, Catarina; Maia, Alexandra S.; Tiritan, Maria Elizabeth; Castro, Paula M. L.; Amorim, Catarina L.

Coastal wastewater treatment plants often face multiple stressors (e.g., pharmaceuticals and oscillating seawater levels) simultaneously, and their combined effects on biological treatment systems are still largely underestimated. In this study, an aerobic granular sludge (AGS) reactor was challenged over a four-month period with wastewater that had daily fluctuations in seawater content (7.5 to 22.5 g/L) and o...


Exploring the biodegradation of PET in mangrove soil and its intermediates by e...

Saidu, Muhammad Bashir; Moreira, Irina S.; Amorim, Catarina L.; Wu, Rongben; Ho, Yuen-Wa; Fang, James Kar-Hei; Castro, Paula M. L.; Gonçalves, David

The biodegradation of Polyethylene terephthalate (PET) is important due to the environmental impact of plastic waste. This study investigates the degradation of PET films in soil microcosms, with and without mangrove plants, and with mangrove plants bioaugmented with a bacterial consortium (Bacillus sp.- GPB12 and Enterococcus sp.- WTP31B-5) while following the evolution of soil microcosm microbiome. The abilit...


Enhancing photosynthetic efficiency and nutrient uptake in Maize (Zea mays L.) ...

Amorim, Catarina L.; Pereira, Sofia I. A.; Sousa, Ana S. S.; Wilfert, Philipp; Loosdrecht, Mark van; Martins, António; Castro, Paula M. L.

Extracellular polymeric substances (EPS) can be recovered from waste aerobic granular sludge (AGS) generated during biological wastewater treatment. These biopolymers are rich in organic carbon and nutrients and can improve the water-holding capacity of soils, making them suitable for agricultural applications.


Wastewater stressors shape the microbiome of aerobic granular sludge

Miranda, Catarina; Maia, Alexandra S.; Tiritan, Maria Elizabeth; Amorim, Catarina L.; Castro, Paula M. L.

Aerobic granular sludge (AGS) is a revolutionary biological treatment technology, which, due to its competitive advantages, has been increasingly implemented in full-scale wastewater treatment plants (WWTPs). WWTPs are frequently challenged by multiple stressors, including pharmaceuticals and fluctuating salinity, which not only increase the complexity of wastewater but also impair the biological treatment perf...


Adaptative biological response of aerobic granular sludge to events of single o...

Alves, Marta; Henriques, Isabel; Castro, Paula M. L.; Amorim, Catarina L.

Wastewater comprises various stressors and their individual and combined immediate effects on aerobic granular sludge (AGS) are still underexplored. In this study, the AGS was exposed for 24 hours to wastewater with varying salt concentrations (up to 30 g NaCl L⁻¹) alongside pharmaceuticals (diclofenac, DCF or carbamazepine, CBZ). Differences in extracellular polymeric substances (EPS) production and compositio...


Meat processing wastewater valorised through microalgae production

Sousa, Ana S. S.; Oliveira, Ana S.; Castro, Paula M. L.; Amorim, Catarina L.

The treatment of meat processing wastewater (MPWW) is crucial to mitigating its environmental impact but remains challenging due to its high organic load and elevated nitrogen and solids content. Microalgae-based processes have been used to treat various types of wastewater, including municipal and industrial sources, offering a sustainable approach that not only cleans wastewater but also produces valuable bio...


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