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Unveiling the spectrum of xylooligosaccharides from lignocellulosic biomasses: ...

Paula, Solciaray C. S. E. de; Pérez, Victor Haber; Sousa, Maria I. S. R. de; Ribeiro, Djully L. G.; Amorim, Cláudia Catarina Oliveira

Lignocellulosic agro-industrial residues are a valuable source for oligosaccharide production, supporting the biorefinery concept and advancing innovative production strategies. This review focuses on xylooligosaccharides (XOS) production from various lignocellulosic residues, focusing on xylan as a primary feedstock. It discusses conventional methods (acid hydrolysis, autohydrolysis, enzymatic hydrolysis) and ...


Epilactose as a promising butyrate-promoter prebiotic via microbiota modulation

Cardoso, Beatriz B.; Amorim, Cláudia Catarina Oliveira; Franco-Duarte, Ricardo; Alves, Joana I.; Barbosa, Sónia G.; Silvério, Sara Isabel Cruz

Epilactose is a disaccharide composed of galactose and mannose, and it is currently considered an under development prebiotic. In this study, we described the prebiotic potential of epilactose by in vitro fermentation using human fecal inocula from individuals following a Mediterranean diet (DM) or a Vegan diet (DV). The prebiotic effect of epilactose was also compared with lactulose and raffinose, and interest...


Agro-industrial wastes as alternative substrates for the production of prebioti...

Braga, Adelaide Correia; Maia, Ana Benedita; Gomes, Daniela Filipa Correia; Costa, João Manuel Rainha; Cardoso, Beatriz Alexandra Batista

Fructooligosaccharides (FOS) are promising prebiotics in the relevant and increasing market of functional food. However, to achieve a more sustainable process, the industrial production of FOS should use cheap substrates. Zymomonas mobilis (ZM) has great potential for the production of FOS due to the presence of native enzymes (levansucrase) capable of metabolizing sucrose. In addition, ZM can use different car...


Valorization of coffee agro-industry residues for prebiotic production by one-p...

Fernandes, Andreia; Cordeiro, Ana; Rodrigues, L. R.; Amorim, Cláudia Catarina Oliveira

Prebiotics are interesting compounds able to modulate the gut microbiota by inducing the growth or activity of beneficial bacteria in the gastrointestinal tract, while the pathogenic ones are inhibited. Several carbohydrates have been considered prebiotics including xylooligosaccarides (XOS). XOS are the only nutraceuticals that can be produced from lignocellulosic biomass. Indeed, XOS can be produced from agro...


Assessing the prebiotic potential of xylooligosaccharides produced by one-step ...

Cordeiro, Ana; Fernandes, Andreia; Sousa, Joana; Cardoso, Beatriz Alexandra Batista; Alves, Joana; Silvério, Sara Isabel Cruz; Peres, António M.

The prebiotic effect is a fundamental concept in the fields of nutrition and gut health, referring to the beneficial effects of specific non-digestible dietary components on the gut microbiota, including xylooligosaccharides (XOS). These compounds function as food sources for beneficial gut bacteria, fostering their growth and activity. In this work, in vitro studies were performed to evaluate the prebiotic pot...


One-step production of a novel prebiotic mixture using Zymomonas mobilis ZM4

Braga, Adelaide Correia; Gomes, Daniela Ferreira Castro; Amorim, Cláudia Catarina Oliveira; Silvério, Sara Isabel Cruz; Alves, Joana I.; Rainha, João

Zymomonas mobilis ZM4 presents interesting features for the biotechnological production of prebiotics. In this work, a Box-Behnken design approach was used to optimize the medium composition and maximize the fructooligosaccharides (FOS) production, in a one-step process. Afterwards, the scale-up to bioreactor allowed a high yield, titer and productivity of FOS (0.52 ± 0.05 gFOS gSuc-1, 156.50 ± 0.58gL-1, 4.89 ±...


Zymomonas mobilis an emerging microbial cell factory to produce prebiotics

Braga, Adelaide Correia; Gomes, Daniela Filipa Correia; Costa, João Manuel Rainha; Amorim, Cláudia Catarina Oliveira; Silvério, Sara Isabel Cruz


Beneficial impact of raffinose on human intestinal microbiota

Amorim, Cláudia Catarina Oliveira; Silvério, Sara Isabel Cruz; Cardoso, B.; Alves, Joana I.; Pereira, M. A.; Rodrigues, L. R.

Until now the prebiotic potential of pure trisaccharide raffinose on human health remains poorly investigated. In particular, studies following the guidelines recently established by the International Scientific Association for Probiotics and Prebiotics are scarce [1]. Raffinose is non-digestible to humans but can be metabolized by colon bacteria [2], therefore being considered a prebiotic candidate. Different ...


Hydrolysates containing xylooligosaccharides produced by different strategies: ...

Gautério, Gabrielle Victoria; Amorim, Cláudia Catarina Oliveira; Silvério, Sara Isabel Cruz; Cardoso, Beatriz B.; Ballesteros, Lina F.; Alves, Joana I.

This study explores the structural characterization, antioxidant and prebiotic activities of hydrolysates containing xylooligosaccharides (XOS) produced by different strategies: direct fermentation of beechwood xylan (FermBX) and enzymatic treatment of beechwood (EnzBX) and rice husk (EnzRH) xylans. EnzBX and EnzRH showed XOS with a backbone of (1 4)-linked-xylopyranosyl residues and branches of arabinose, gala...


Prebiotic potential of hydrolysates containing xylooligosaccharides produced by...

Gautério, Gabrielle V.; Amorim, Cláudia Catarina Oliveira; Silvério, Sara Isabel Cruz; Alves, Joana I.; Pereira, M. Alcina; Kalil, Susana

Xylooligosaccharides (XOS) are considered emergent prebiotics with potential for several industrial applications. Here, we investigated the prebiotic activity of XOS-containing hydrolysates produced by different processes: direct fermentation of beechwood xylan (BXL) and enzymatic treatment of beechwood (XLH) or rice husk (RHH) xylans. BXL was obtained by fermentation of beechwood xylan (2.5 g/L increased up to...


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