30 documents found, page 1 of 3
Chemical Characterization and Differential Lipid-Modulating Effects of Selected...
Amorim, Ricardo; Marques, Mário Pedro; Melim, Catarina; Varela, Carla; Sardão, Vilma A.; Teixeira, José; Dias, Maria Inês; Barros, LillianCôa Valley, located in the northeast of Portugal, harbors more than 500 medicinal plant species. Among them, four species stand out due to their traditional uses: Equisetum ramosissimum Desf. (hemorrhages, urethritis, hepatitis), Rumex scutatus L. subsp. induratus (Boiss. and Reut.) Malag. (inflammation, constipation), Geranium purpureum Vill., and Geranium lucidum L. (pain relief, gastric issues). Given their ...
RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabol...
Afonso, Marta B.; Islam, Tawhidul; Magusto, Julie; Amorim, Ricardo; Lenoir, Véronique; Simões, Rui F.; Teixeira, José; Silva, Liana C.Background and Aims: Receptor‐interacting protein kinase 3 (RIPK3) mediates NAFLD progression, but its metabolic function is unclear. Here, we aimed to investigate the role of RIPK3 in modulating mitochondria function, coupled with lipid droplet (LD) architecture in NAFLD. Approach and Results: Functional studies evaluating mitochondria and LD biology were performed in wild‐type (WT) and Ripk3−/− mice fed a cho...
From Non-Alcoholic Fatty Liver to Hepatocellular Carcinoma: A Story of (Mal)Ada...
Amorim, Ricardo; Magalhães, Carina C.; Borges, Fernanda; Oliveira, Paulo J.; Teixeira, JoséNon-alcoholic fatty liver disease (NAFLD) is a global pandemic affecting 25% of the world's population and is a serious health and economic concern worldwide. NAFLD is mainly the result of unhealthy dietary habits combined with sedentary lifestyle, although some genetic contributions to NAFLD have been documented. NAFLD is characterized by the excessive accumulation of triglycerides (TGs) in hepatocytes and enc...
Mitochondria-targeted anti-oxidant AntiOxCIN4 improved liver steatosis in Weste...
Amorim, Ricardo; Simões, Inês C. M.; Teixeira, José; Cagide, Fernando; Potes, Yaiza; Soares, Pedro; Carvalho, Adriana; Tavares, Ludgero C.Non-alcoholic fatty liver disease (NAFLD) is a health concern affecting 24% of the population worldwide. Although the pathophysiologic mechanisms underlying disease are not fully clarified, mitochondrial dysfunction and oxidative stress are key players in disease progression. Consequently, efforts to develop more efficient pharmacologic strategies targeting mitochondria for NAFLD prevention/treatment are underw...
The Alterations of Mitochondrial Function during NAFLD Progression-An Independe...
Simões, Inês C M; Amorim, Ricardo; Teixeira, José; Karkucinska-Wieckowska, Agnieszka; Carvalho, Adriana; Pereira, Susana P; Simões, Rui F.The progression of non-alcoholic fatty liver (NAFL) into non-alcoholic steatohepatitis implicates multiple mechanisms, chief of which is mitochondrial dysfunction. However, the sequence of events underlying mitochondrial failure are still poorly clarified. In this work, male C57BL/6J mice were fed with a high-fat plus high-sucrose diet for 16, 20, 22, and 24 weeks to induce NAFL. Up to the 20th week, an early m...
Exploratory Data Analysis of Cell and Mitochondrial High-Fat, High-Sugar Toxici...
Amorim, Ricardo; Simões, Inês C. M.; Veloso, Caroline; Carvalho, Adriana; Simões, Rui F.; Pereira, Francisco B.; Thiel, Theresa; Normann, AndreaNon-alcoholic steatohepatitis (NASH), one of the deleterious stages of non-alcoholic fatty liver disease, remains a significant cause of liver-related morbidity and mortality worldwide. In the current work, we used an exploratory data analysis to investigate time-dependent cellular and mitochondrial effects of different supra-physiological fatty acids (FA) overload strategies, in the presence or absence of fruc...
Photodynamic therapy at low-light fluence rate: "in vitro" assays on colon canc...
Rodrigues, J. A.; Amorim, Ricardo; Silva, M. F.; Baltazar, Fátima; Wolffenbuttel, R. F.; Correia, J. H.This paper presents the results of in vitro photodynamic therapy assays on RKO and HCT-15 cell lines. The envisaged implementation is in autonomous medical microdevices, such as endoscopic capsules for clinical treatment of several types of gastrointestinal tract tumors. Because of their very limited device volume, light fluence and fluence rate needed to destroy tumor cells should be minimized. Foscan or meta-...
Hydroxybenzoic Acid Derivatives as Dual-Target Ligands: Mitochondriotropic Anti...
Oliveira, Catarina; Cagide, Fernando; Teixeira, José; Amorim, Ricardo; Sequeira, Lisa; Mesiti, Francesco; Silva, Tiago; Garrido, Jorge; Remião, FernandoAlzheimer's disease (AD) is a multifactorial age-related disease associated with oxidative stress (OS) and impaired cholinergic transmission. Accordingly, targeting mitochondrial OS and restoring cholinergic transmission can be an effective therapeutic strategy toward AD. Herein, we report for the first time dual-target hydroxybenzoic acid (HBAc) derivatives acting as mitochondriotropic antioxidants and choline...
Discovery of a new mitochondria permeability transition pore (mPTP) inhibitor b...
Teixeira, José; Oliveira, Catarina; Cagide, Fernando; Amorim, Ricardo; Garrido, Jorge; Borges, Fernanda; Oliveira, Paulo J.Pharmacological interventions targeting mitochondria present several barriers for a complete efficacy. Therefore, a new mitochondriotropic antioxidant (AntiOxBEN3) based on the dietary antioxidant gallic acid was developed. AntiOxBEN3 accumulated several thousand-fold inside isolated rat liver mitochondria, without causing disruption of the oxidative phosphorylation apparatus, as seen by the unchanged respirato...
Development of hydroxybenzoic-based platforms as a solution to deliver dietary ...
Teixeira, José; Oliveira, Catarina; Amorim, Ricardo; Cagide, Fernando; Garrido, Jorge; Ribeiro, José A.; Pereira, Carlos M.; Silva, António F. M.Oxidative stress and mitochondrial dysfunction have been associated with metabolic and age-related diseases. Thus, the prevention of mitochondrial oxidative damage is nowadays a recognized pharmacological strategy to delay disease progression. Epidemiological studies suggested an association between the consumption of polyphenol-rich diet and the prevention of different pathologies, including diseases with a mi...