24 documents found, page 1 of 3
Supercritical CO decellularized-ECM derived coreshell hydrogel for functional β...
Pazmino, Carlos; Sá, Simone; Azevedo, Ana; Oliveira, Ana L.; Amorim, SaraIntroduction: Type 1 Diabetes Mellitus (T1DM) is an autoimmune disease that destroys insulinproducing pancreatic cells, affecting over 9 million people worldwide. While insulin therapy is standard, it often leads to poor glucose control or hypoglycemia. Pancreatic islet transplantation shows promise but faces graft survival challenges. This study develops a permeable, pro-angiogenic immune-isolation hydrogel us...
Core–shell hydrogel based on supercritical CO2-decellularized pancreatic ECM fo...
Pazmino, Carlos; Sá, Simone; Amorim, Sara; Oliveira, Ana L.Type 1 Diabetes Mellitus (T1DM) affects over 9 million people worldwide and is characterized by autoimmune destruction of insulin-producing pancreatic ?-cells. While exogenous insulin remains the primary treatment, inaccurate dosing often leads to poor glycaemic control. Pancreatic islet transplantation offers a potential curative approach; however, long-term success is hindered by immune rejection and poor gra...
Patterned glycopeptide-based supramolecular hydrogel promotes the alignment and...
Castro, Vânia Isabel Baptista; Amorim, Sara; Caballero, David; Abreu, Catarina M.; Kundu, Subhas C; Reis, R. L.; Pashkuleva, I.; Pires, R. A.The functional restoration of a damaged cardiac tissue relies on a synchronized contractile capacity of exogenous and/or endogenous cardiomyocytes, which is challenging to achieve. Here, we explored the potential of the short glycopeptide diphenylalanine glucosamine-6-sulfate (FFGlcN6S) conjugated with an aromatic moiety, namely fluorenylmethoxycarbonyl (Fmoc), to enhance cardiac tissue regeneration. At physiol...
Supercritical CO2-assisted decellularization: advanced pancreatic tissue platfo...
Sá, Simone C.; Pazmino, Carlos; Sá, Joana; Amorim, Sara; Ribeiro, Viviana P.; Costa, Raquel; Oliveira, Ana L.Introduction: Decellularization is a procedure that aims to remove the cellular and antigenic material of a tissue while preserving its extracellular matrix (ECM) and biological properties. Decellularized ECM offers a natural microenvironment with significant potential for use in cell-based therapies and tissue regeneration [1]. Different protocols have been employed to decellularize tissues and organs. However...
Bio-based hydrogel for beta cell islet transplantation in type 1 diabetes therapy
Oliveira, Ana L.; Pazmino, Carlos; Sá, Simone; Amorim, SaraObjectives: Type 1 Diabetes Mellitus (T1DM) is a chronic autoimmune disease that destroys insulin-producing pancreatic cells, affecting over 9 million people globally. The primary treatment involves insulin therapy, but improper delivery can lead to poor glucose control or hypoglycemia. Pancreatic islet transplantation offers a potential alternative, but long-term graft survival remains a challenge. This study ...
Decellularized cardiac tissue for the development of non-immunogenic EPSs-rich ...
Pazmino, Carlos; Duarte, Marta M.; Oliveira, Ana L.; Amorim, SaraIntroduction: Cardiovascular diseases account for 32% of the global deaths, comprising different pathologies related to heart failure. The heart self-regeneration is limited, which is associated to the highest mortality and morbidity rates of cardiovascular diseases. To bridge this gap, we developed a decellularized extracellular matrix (dECM)-based cardiac patch functionalized with sulfated exopolysaccharides ...
Decellularized matrix-based hydrogel for targeted beta cell islet delivery in t...
Pazmino, Carlos; Sá, Simone; Amorim, Sara; Oliveira, Ana L.Introduction: Type 1 Diabetes Mellitus (T1DM) affects over 9 million people worldwide and is marked by autoimmune destruction of pancreatic β-cells, which produce insulin. Current treatment relies on exogenous insulin, but inaccurate delivery often causes poor glycemic control or hypoglycemia. Islet transplantation offers a promising alternative, though long-term graft survival remains a challenge. This work pr...
Unlocking the potential of supercritical CO₂ for an efficient pancreatic tissue...
Sá, Simone C.; Pazmino, Carlos; Sá, Joana; Amorim, Sara; Ribeiro, Viviana P.; Costa, Raquel; Oliveira, Ana L.Introduction: Tissue decellularization is a procedure that removes cellular and antigenic material from specific tissues, while preserving the extracellular matrix (ECM), with conditions tailored to the tissue's properties. The decellularized ECM is a promising material for cell-based therapies or for tissue defect repair through the development of scaffolds and membranes, among others. Pancreas is an organ tha...
I-CARE: implantable decellularized-based cardiac patch for cardiac tissue repair
Oliveira, Ana L.; Amorim, SaraIntroduction: Cardiovascular diseases account for 32% of the global deaths, comprising different pathologies related to heart failure. [1] The heart self-regeneration is limited, which is associated to the highest mortality and morbidity rates of cardiovascular diseases. The incapacity of endogenous regeneration reduces therapeutic approaches to the relief of symptoms rather than restoring the proper function o...
Fucoidan-coated silica nanoparticles promote the differentiation of human mesen...
Amorim, Sara; Dudik, Olesia; Soares da Costa, Diana; Reis, R. L.; Silva, Tiago H.; Pires, R. A.Silica nanoparticles (SiNPs) are widely used in biomedical applications, such as cancer therapy/diagnosis or tissue engineering and regenerative medicine. Herein, we synthesized SiNPs and modified them with sulfonic acid groups (by organosilylation followed by oxidation) or a sulfated polysaccharide (i.e., fucoidan, a seaweed biopolymer, by using electrostatic surface immobilization) due to the known capacity o...