20 documents found, page 1 of 2

Supercritical CO2-assisted decellularization: advanced pancreatic tissue platfo...

Introduction: Decellularization is a procedure that aims to remove the cellular and antigenic material of a tissue while preserving its extracellular matrix (ECM) and biological properties. Decellularized ECM offers a natural microenvironment with significant potential for use in cell-based therapies and tissue regeneration [1]. Different protocols have been employed to decellularize tissues and organs. However...

Patterned glycopeptide-based supramolecular hydrogel promotes the alignment and...

The functional restoration of a damaged cardiac tissue relies on a synchronized contractile capacity of exogenous and/or endogenous cardiomyocytes, which is challenging to achieve. Here, we explored the potential of the short glycopeptide diphenylalanine glucosamine-6-sulfate (FFGlcN6S) conjugated with an aromatic moiety, namely fluorenylmethoxycarbonyl (Fmoc), to enhance cardiac tissue regeneration. At physiol...

Unlocking the potential of supercritical CO₂ for an efficient pancreatic tissue...

Decellularization, the process of removing cells from a tissue or organ while preserving the extracellular matrix (ECM), often employs harsh chemical solvents, compromising ECM integrity. This is particularly damaging in case of soft tissues. Supercritical carbon dioxide (scCO2), achieved at 31ºC and 74Bar, is an alternative solvent which presents high transfer rates and diffusivity while being chemically inert...

I-CARE: implantable decellularized-based cardiac patch for cardiac tissue repair

Introduction: Cardiovascular diseases account for 32% of the global deaths, comprising different pathologies related to heart failure. [1] The heart self-regeneration is limited, which is associated to the highest mortality and morbidity rates of cardiovascular diseases. The incapacity of endogenous regeneration reduces therapeutic approaches to the relief of symptoms rather than restoring the proper function o...

Unlocking the potential of supercritical CO₂ for an efficient pancreatic tissue...

Introduction: Tissue decellularization is a procedure that removes cellular and antigenic material from specific tissues, while preserving the extracellular matrix (ECM), with conditions tailored to the tissue's properties. The decellularized ECM is a promising material for cell-based therapies or for tissue defect repair through the development of scaffolds and membranes, among others. Pancreas is an organ tha...

Melodic and vocal performance of school-aged children from disadvantaged backgr...

Fucoidan-coated silica nanoparticles promote the differentiation of human mesen...

Silica nanoparticles (SiNPs) are widely used in biomedical applications, such as cancer therapy/diagnosis or tissue engineering and regenerative medicine. Herein, we synthesized SiNPs and modified them with sulfonic acid groups (by organosilylation followed by oxidation) or a sulfated polysaccharide (i.e., fucoidan, a seaweed biopolymer, by using electrostatic surface immobilization) due to the known capacity o...

Psychometric properties of the portuguese version of the occupational fatigue e...

Reliable and valid instruments are essential for understanding fatigue in occupational settings. This study analyzed the psychometric properties of the Portuguese version of the Occupational Fatigue Exhaustion/Recovery (OFER). A cross-sectional study was conducted with 260 workers (M = 46.52 ± 13.16 years) from a textile industry, exposed to shift work. Convergent and discriminant validity, internal consistency...

3D hydrogel mimics of the tumor microenvironment: the interplay among hyaluroni...

The present work reports on a 3D model of the tumor microenvironment that contains hyaluronic acid (HA) and alginate, and demonstrates the utility of this model to study the effect of HA size on the crosstalk between cancer cells and mesenchymal stem cells (MSCs). The system incorporates a core that contains HA of specific size (i.e. 6.4, 741 or 1500 kDa) with encapsulated epithelial MKN45 cancer cells and a s...

Bioactivity of biosilica obtained from North Atlantic deep-sea sponges

Demosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalysed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia barretti (GB), Stelletta normani (SN), Axinella infundibuliformis (AI) and Phakellia ventilabrum (PV) to explore the...