2 documents found, page 1 of 1
Simultaneous quantification of lycopene, β-carotene, retinol and α -tocopherol ...
Charão,Mariele F.; Moro,Angela M.; Brucker,Natália; Bulcão,Rachel P.; Baierle,Marília; Freitas,Fernando; Durgante,Juliano; Nascimento,SabrinaA method for the simultaneous quantification of lycopene, β-carotene, retinol and α-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at -20°C for...
Quantificação sérica de vitamina C por CLAE-UV e estudo de estabilidade
Baierle,Marília; Bairros,André de; Moreira,Ana Paula; Bulcão,Rachel; Roehrs,Miguel; Freitas,Fernando de; Durgante,Juliano; Brucker,NatáliaVitamin C, an exogenous antioxidant, is essential to human health. In this study, a method was validated to serum vitamin C quantification by HPLC-UV. Its stability with and without the use of tris [2-carboxy-ethyl] phosphine hydrochloride (TCEP), at -20 and -80 °C, in serum and supernatant were also evaluated. Analysis showed r² > 0.99, precision CV% < 15% and % bias < 15%, being linear, precise and accurat...