4 documents found, page 1 of 1
Field randomized trial to evaluate the efficacy of the Leish - Tec vaccine agai...
Silva, Shara Regina; Feres, Ana Maria Leonardi Tibúrcio; Silva, João Carlos França da; Dias, Edelberto Santos; Michalsky, Érika MonteiroBackground: A canine vaccine remains a promising approach for effective control of visceral leishmaniasis(VL), given its complex epidemiology in areas where zoonotic VL is prevalent. Leish-Tec®is a recombi-nant vaccine, based on the Leishmania A2 antigen, against canine VL (CVL). It is, since 2014, the singlecommercial vaccine licensed in Brazil. Here, Leish-Tec®efficacy was estimated through a randomizedfield ...
Novel recombinant multiepitope proteins for the diagnosis of asymptomatic Leish...
Faria, Angélica Rosa; Veloso, Luciano de Castro; Vital, Wendel Coura; Reis, Alexandre Barbosa; Damasceno, Leonardo Miranda; Gazzinelli, Ricardo TostesBackground: Visceral leishmaniasis is the most severe form of leishmaniasis. Worldwide, approximately 20% of zoonotic human visceral leishmaniasis is caused by Leishmania infantum, also known as Leishmania chagasi in Latin America. Current diagnostic methods are not accurate enough to identify Leishmania-infected animals and may compromise the effectiveness of disease control. Therefore, we aimed to produce and...
Comparative evaluation of phenol and thimerosal as preservatives for a candidat...
Mayrink, Wilson; Tavares, Carlos Alberto Pereira; Deus, Rosângela Barbosa de; Pinheiro, Melina Barros; Guimarães, Tânia Mara Pinto DabésFor decades thimerosal has been used as a preservative in the candidate vaccine for cutaneous leishmaniasis, which was developed by Mayrink et al. The use of thimerosal in humans has been banned due to its mercury content. This study addresses the standardization of phenol as a new candidate vaccine preservative. We have found that the proteolytic activity was abolished when the test was conducted using the can...
Use of PCR–RFLP to identify Leishmania species in naturally-infected dogs.
Andrade, Hélida Monteiro de; Reis, Alexandre Barbosa; Santos, Sara Lopes dos; Volpini, Ângela Cristina; Marques, Marcos José; Romanha, Alvaro JoséTissue imprints on Giemsa stained slides from dogs were used to investigate the presence of Leishmania amastigotes by either optical microscopy (OM) or Polymerase chain reaction (PCR) detection of DNA. Samples from skin, spleen, lymph node, liver and bone marrow from a Leishmaniasis endemic area dogs where Leishmania ( Leishmania ) chagasi and Leishmania ( Viannia) braziliensis are sympatric were studied. Dogs ...