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Optimization of live prey enrichment media for rearing juvenile short-snouted s...

Palma, Jorge; Lima, Ricardo; Andrade, José Pedro; Lança, M.J.

This study aimed to determine a nutritionally adequate feeding protocol for Hippocampus hippocampus juveniles. In the experimental trial, seahorses were fed copepods from 0–7 days post parturition (DPP) and, from 8–28 DPP, four different dietary treatments: (copepods (control diet) (Cop); microalgae-enriched Artemia with a docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) ratio of 2:1 (ArtDHA/EPA); microal...


A crown for each monarch: a distinguishable pattern using photo-identification

Correia, Miguel; Antunes, Daniela; Andrade, José Pedro; Palma, Jorge

Photo-identification has been proven to be a successful individual recognition tool in seahorse species (e.g., long-snout seahorse Hippocampus reidi (Ginsburg, 1933) and long-snouted seahorse Hippocampus guttulatus (Cuvier, 1829)). Its use was deemed valuable for the assessment of wild populations and to understand variations in abundance over time when capture-recapture methods are needed. In this study, a com...


Control of zootechnology leads to improved Cuttlefish (Sepia officinalis, L.) r...

Capaz, Juan Carlos; Hernández-Brooke, Daniel; Balvet, Safia; Couto, Ana T.; Alves, Alexandra; Baptista Gonçalves, Rui; Frias, Paulo A.

Cephalopods are gaining momentum as an alternate group for aquaculture species diversification, not only because they are a good food source (highly appreciated in some worldwide markets) but they also have the potential to quickly reach a market size. However, there are some bottlenecks impeding the transition of culture technology from the laboratory to industry. One is related to control over reproduction in...


Seal and polar bear behavioral response to an icebreaker vessel in northwest Gr...

Lomac-MacNair, Kate; Andrade, José Pedro; Esteves, Eduardo

Icebreaker vessels are important scientific tools, enabling access and research within the polar regions of the world, including the High Arctic. These vessels have the potential to overlap with marine mammal habitats in infrequently studied areas. Marine mammal behavioral responses to icebreaker vessel presence and distance at which responses occur are not well documented or understood. During the Petermann 20...


Retinal differentiation in syngnathids: comparison in the developmental rate an...

Alvarez-Hernan, Guadalupe; Andrade, José Pedro; Escarabajal-Blazquez, Laura; Blasco, Manuel; Solana-Fajardo, Jorge; Martin-Partido, Gervasio

The altricial-precocial spectrum describes the degree of morphological maturation of offspring at the moment of hatching. In fishes, precocial species develop all their structures at early stages of embryogenesis and larvae hatch at an advanced stage of development, while altricial species hatch at a less developed stage. The timing of retinal development also varies significantly between precocial and altricia...


Interrelationship between contractility, protein synthesis and metabolism in ma...

Lamarre, Simon G.; MacCormack, Tyson J.; Bourloutski, Émilie; Callaghan, Neal I.; Pinto, Vanessa; Andrade, José Pedro; Sykes, António

Young juvenile cuttlefish (Sepia officinalis) can grow at rates as high as 12% body weight per day. How the metabolic demands of such a massive growth rate impacts muscle performance that competes for ATP is unknown. Here, we integrate aspects of contractility, protein synthesis, and energy metabolism in mantle of specimens weighing 1.1 g to lend insight into the processes. Isolated mantle muscle preparations w...


Effects of anthropogenic noise as a source of acoustic stress in wild populatio...

Palma, Jorge; Magalhães, Marisa Gonçalves; Correia, Miguel; Andrade, José Pedro

This study evaluated the effects of underwater noise as a source of acoustic stress in the wild populations of Hippocampus guttulatus in the Ria Formosa, south Portugal. Two different scenarios of underwater noise were tested: transient motor boat sound (63.4-127.6 dB) and constant sound produced by the motor of the boat directly above the animals (up to 137.1 dB). Observations were obtained in the wild between...


Effect of of Artemia inherent fatty acid metabolism on the bioavailability of e...

B Reis, Diana; Acosta, Nieves G.; Almansa, Eduardo; Garrido, Diego; Andrade, José Pedro; Sykes, Antonio V.; Rodriguez, Covadonga

The aim of the present study was to determine the effect of Artemia metanauplii endogenous fatty acid (FA) metabolism in the actual availability of dietary essential fatty acids (EFA) for Octopus vulgaris paralarvae development. To this end, both Artemia metanauplii inherent radiolabelled FA metabolism and the Octopus vulgaris paralarvae FA metabolism, after being fed with Artemia metanauplii incubated with rad...


The impact of dietary supplementation with astaxanthin on egg quality and growt...

Palma, Jorge; Andrade, José Pedro; Bureau, D. P.

This study investigated the effect of dietary astaxanthin supplementation on egg quality and juvenile growth of long snout seahorse (Hippocampus guttulatus). Captive breed seahorse broodstock were fed four diets composed of frozen shrimp [Atlantic ditch shrimp, Palaemonetes varians) used as a vector to deliver artificial diets with increasing levels of astaxanthin (0, 75, 100 and 125 mg kg(-1) dry weight)]. The...


Comparative study on fatty acid metabolism of early stages of two crustacean sp...

Reis, Diana; Acosta, Nieves G.; Almansa, Eduardo; Navarro, Juan C.; Tocher, Douglas R.; Andrade, José Pedro; Sykes, António V.; Rodríguez, Covadonga

The present study compared the lipid composition and in vivo capability of Artemia sp. metanauplii (the main live prey used in aquaculture) and Grapsus adscensionis zoeae (as a wild zooplankton model) to metabolise unsaturated fatty acids. The two species were incubated in vivo with 0.3μM of individual [1-14C]fatty acids (FA) including 18:1n-9, 18:2n-6, 18:3n-3, 20:4n-6 (ARA), 20:5n-3 (EPA) and 22:6n-3 (DHA) bo...


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