10 documents found, page 1 of 1
CuMV VLPs Containing the RBM from SARS-CoV-2 Spike Protein Drive Dendritic Cell...
Sebastião, Ana Isabel; Mateus, Daniela; Carrascal, Mylène A.; Sousa, Cátia; Cortes, Luísa; Bachmann, Martin F; do Carmo, Anália; Matos, Ana M.Dendritic cells (DCs) are the most specialized and proficient antigen-presenting cells. They bridge innate and adaptive immunity and display a powerful capacity to prime antigen-specific T cells. The interaction of DCs with the receptor-binding domain of the spike (S) protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a pivotal step to induce effective immunity against the S protei...
YES1 Kinase Mediates the Membrane Removal of Rescued F508del-CFTR in Airway Cel...
Barros, Patrícia; Matos, Ana M.; Matos, Paulo; Jordan, PeterRecent developments in CFTR modulator drugs have had a significant transformational effect on the treatment of individuals with Cystic Fibrosis (CF) who carry the most frequent F508del- CFTR mutation in at least one allele. However, the clinical effects of these revolutionary drugs remain limited by their inability to fully restore the plasma membrane (PM) stability of the rescued mutant channels. Here, we shed...
Treatment of Polarized Cystic Fibrosis Airway Cells With HGF Prevents VX-661-Re...
Matos, Ana M.; Jordan, Peter; Matos, PauloCystic fibrosis (CF), the most common inherited disease in Caucasians, is caused by mutations in CFTR, the most frequent of which is F508del. F508del causes ER retention and degradation of the mutant CFTR protein, but also defective channel gating and decreased half-life at the plasma membrane. Despite the recent successes with small molecule CFTR modulator drugs, the folding-corrector/gating-potentiator drug c...
Effect of Thermal Drying and Chemical Treatments with Wastes on Microbiological...
Santos, Andreia F.; Santos, Cátia P.; Matos, Ana M.; Cardoso, Olga; Quina, Margarida J.This work aims to evaluate the microbiological contamination of sewage sludge (SS) collected in urban wastewater treatment plants (WWTP) from Portugal. Two types of SS were considered: urban mixed (UM) and from anaerobic digestion (AD). The two types of samples were characterized in relation to the main physical and chemical parameters, as well as the microbiological contamination (Escherichia coli and Salmonel...
Multiscale multifactorial approaches for engineering tendon substitutes
Gonçalves, Ana I.; Rodrigues, Márcia T.; Matos, Ana M.; Almeida, Helena; Gomez-Florit, Manuel; Domingues, Rui Miguel Andrade; Gomes, Manuela E.The physiology of tendons and the continuous strains experienced daily make tendons very prone to injury. Excessive and prolonged loading forces and aging also contribute to the onset and progression of tendon injuries, and conventional treatments have limited efficacy in restoring tendon biomechanics. Tissue engineering and regenerative medicine (TERM) approaches hold the promise to provide therapeutic solutio...
Magnetic biomaterials and nano-instructive tools as mediators of tendon mechano...
Matos, Ana M.; Gonçalves, Ana I.; El Haj, Alicia J.; Gomes, Manuela E.Tendon tissues connect muscle to bone allowing the transmission of forces resulting in joint movement. Tendon injuries are prevalent in society and the impact on public health is of utmost concern. Thus, clinical options for tendon treatments are in demand, and tissue engineering aims to provide reliable and successful long-term regenerative solutions. Moreover, the possibility of regulating cell fate by trigge...
Inhibition of calpain 1 restores plasma membrane stability to pharmacologically...
Matos, Ana M.; Pinto, Francisco R.; Barros, Patrícia; Amaral, Margarida D.; Pepperkok, Rainer; Matos, PauloCystic fibrosis (CF) is a genetic disease caused by mutations in the gene encoding CF transmembrane conductance regulator (CFTR), a chloride channel normally expressed at the surface of epithelial cells. The most frequent mutation, resulting in Phe-508 deletion, causes CFTR misfolding and its premature degradation. Low temperature or pharmacological correctors can partly rescue the Phe508del-CFTR processing def...
Ozone and Photocatalytic Processes for Pathogens Removal from Water: A Review
Gomes, João; Matos, Ana M.; Gmurek, Marta; Quinta-Ferreira, R. M.; Martins, Rui C.The search for alternative water sources is pushing to the reuse of treated water coming from municipal wastewater treatment plants. However, this requires that tightened standards be fulfilled. Among them is the microbiological safety of reused water. Although chlorination is the mostly applied disinfection system, it presents several disadvantages, such as the high doses required and the possibility of format...
Removal of Enteric Pathogens from Real Wastewater Using Single and Catalytic Oz...
Gomes, João; Frasson, Danilo; Quinta-Ferreira, Rosa M.; Matos, Ana M.; Martins, Rui C.Water scarcity is one of the main problems of this century. Water reclamation appears as an alternative due to the reuse of treated wastewater. Therefore, effluents treatment technologies (activated sludge, rotary biological discs, percolating beds) must be improved since they are not able to remove emerging contaminants such as enteric pathogens (bacteria and virus). These pollutants are difficult to remove fr...
Prolonged co-treatment with HGF sustains epithelial integrity and improves phar...
Matos, Ana M.; Gomes-Duarte, Andreia; Faria, Márcia; Barros, Patrícia; Jordan, Peter; Amaral, Margarida D.; Matos, PauloCystic fibrosis (CF), the most common inherited disease in Caucasians, is caused by mutations in the CFTR chloride channel, the most frequent of which is Phe508del. Phe508del causes not only intracellular retention and premature degradation of the mutant CFTR protein, but also defective channel gating and decreased half-life when experimentally rescued to the plasma membrane (PM). Despite recent successes in th...