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Preliminary report of HIV and Toxoplasma gondii occurrence in pregnant women fr...

Sitoe, Sonia Paula Benedito Luis; Rafael, Bernardete; Meireles, Luciana Regina; Andrade Jr., Heitor Franco de; Thompson, Ricardo

Toxoplasmosis, a protozoan disease, causes severe disease in fetuses during pregnancy and deadly encephalitis in HIV patients. There are several studies on its seroprevalence around the world, but studies focusing on African countries are limited in number and mostly anecdotal. We studied two groups of samples from Mozambique by ELISA, using serum samples from 150 pregnant women and six Cerebrospinal fluid (CSF...

Date: 2010   |   Origin: Oasisbr


Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women

Macre, Miriam de Souza; Pires, Márcio; Meireles, Luciana Regina; Angel, Sérgio O.; Andrade Jr., Heitor Franco de

Toxoplasma gondii causes severe fetal disease during acute infection in pregnant women, thus demanding early diagnosis for effective treatment and fetus preservation. Fetal tests are inefficient and risky, and diagnosis is based on maternal IgM serology, which had weak screening ability due to increased sensitivity, with alternative IgG avidity tests. Here, we performed ELISA and avidity assays using a recombin...

Date: 2009   |   Origin: Oasisbr

Single step polymerase chain reaction (PCR) for the diagnosis of the Leishmania...

Paiva, Byanca Regina; Passos, Luciana Neves; Falqueto, Aloisio; Malafronte, Rosely dos S.; Andrade Jr., Heitor Franco de

No Brasil, o principal agente etiológico da leishmaniose, apresentando freqüentemente comprometimento das mucosas, pertence ao subgênero (Viannia). A conduta terapêutica no tratamento da leishmaniose depende de seu diagnóstico parasitológico e os métodos clássicos restringem sua identificação. Neste trabalho, descrevemos uma reação de PCR, utilizando primers desenhados a partir de seqüências repetitivas de mini...

Date: 2004   |   Origin: Oasisbr

Serological survey of antibodies to Toxoplasma gondii in food animals from São ...

Meireles, Luciana Regina; Galisteo Jr., Andrés Jimenez; Andrade Jr., Heitor Franco de

Toxoplasmosis is one of the most prevalent parasitic infections of man and livestock, and its transmission has usually been attributed to ingestion of undercooked or raw meat from infected livestock, with the infection rate in those animals being an important risk predictor of human disease, high in Brazil and São Paulo State. Looking for this public health problem, we tested serum samples from cattle, goat, sh...

Date: 2003   |   Origin: Oasisbr

Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni wo...

Pinto, Pedro Luiz Silva; Kanamura, Herminia Y.; Silva, Rita Maria; Rossi, Cristina Renata Nardoto; Andrade Jr., Heitor Franco de; Amato Neto, Vicente

Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies ag...

Date: 1995   |   Origin: Oasisbr

Malária experimental: contaminação de cepas e animais de biotério por eperythro...

Andrade Jr., Heitor Franco de; Laurenti, Márcia Dalastra; Goto, Hiro; Duarte, Maria Irma Seixas; Corbett, Carlos Eduardo Pereira

This report describes an accidental contamination of a murine malaria strain (Plasmodium berghei) by a hemoparasite (Eperythrozoon coccoides) wich resulted in important modification of the experimental malarial infection behaviour. The hemoparasite "was detected by optical and electron microscopy, and the contamination was attributed to rodents usually employed in the strain maintenance, obtained from the same ...

Date: 1986   |   Origin: Oasisbr

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