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Cloning and expression of Neospora caninum rhoptry 2 and its potential as an im...

Santos Junior,Alceu G. dos; Conrad,Neida Lucia; Piraine,Renan Eugênio A.; Cunha,Rodrigo C.; Andreotti,Renato; Leite,Fábio P.L.

ABSTRACT: The protozoan Neospora caninum is known worldwide as one of the main causes of abortion in cattle. During infection, rhoptry proteins present in the apical complex of the parasite play important roles in adhesion and parasitophorous vacuole formation. The use of N. caninum ROP2 in experimental vaccines has shown promising protective results. In our study we performed cloning and expression in Escheric...

Date: 2021   |   Origin: Oasisbr

Serological evaluation of Neospora caninum in pregnant women treated at referra...

Duarte,Pâmella Oliveira; Csordas,Bárbara Guimarães; Oshiro,Leandra Marla; Higa,Leandro de Oliveira Souza; Zimmermann,Namor Pinheiro

Abstract Neospora caninum is an obligate intracellular protozoan with canids (Canis domesticus, Canis lupus dingo, Canis latrans, Canis lupus) as its definitive hosts. The objective of this study was to detect anti-N. caninum antibodies in pregnant women seen at referral center for prenatal screening in the state of state Mato Grosso do Sul, Brazil. A total of 188 serum samples from pregnant women provided by t...

Date: 2020   |   Origin: Oasisbr

Amblyomma scalpturatum Neumann, 1906 (Acari: Ixodidae): confirmation in Acre St...

Aguirre,André de Abreu Rangel; Rodrigues,Vinícius da Silva; Costa,Ivaneide Nunes da; Garcia,Marcos Valério; Csordas,Bárbara Guimarães; Andreotti,Renato

Abstract Amblyomma scalpturatum is a tick species that is unique to South America. It is commonly associated with the Amazon biome and has been reported in some Brazilian states. This tick species exhibits host specificity: it parasitizes tapirs and suidae. Its role in transmitting pathogens to humans is still unknown. Amblyomma scalpturatum is known to be a human-biting tick; however, there is only one report ...

Date: 2019   |   Origin: Oasisbr

Biological parameters of Amblyomma coelebs Neumann, 1906 (Acari: Ixodidae) unde...

Aguirre,André de Abreu Rangel; Rodrigues,Vinicius da Silva; Costa,Ivaneide Nunes da; Garcia,Marcos Valerio; Higa,Leandro de Oliveira Souza

Abstract One generation of Amblyomma coelebs life cycle under experimental conditions was evaluated. Ten tick pairs were allowed to feed on rabbits under laboratory conditions (LC), resulting six engorged females with a mean weight of 1,403.9 mg. Two females were maintained in a forest reserve under natural conditions (NC), and four were maintained in incubators (LC). The engorgement period lasted 10.33 days. P...

Date: 2018   |   Origin: Oasisbr

Wildlife species, Ixodid fauna and new host records for ticks in an Amazon fore...

Zimmermann,Namor Pinheiro; Aguirre,André de Abreu Rangel; Rodrigues,Vinicius da Silva; Garcia,Marcos Valério; Medeiros,Jansen Fernandes

Abstract The objective of this work was to evaluate the diversity of ticks associated with free-living animals and to investigate new host records for ticks. Ticks were collected from animals rescued during the flood of the Jamari River in the municipality of Ariquemes, state of Rondônia, North Region of Brazil. A total of 39 animals were captured, out of which 10 were amphibians, 19 were reptiles and 10 were m...

Date: 2018   |   Origin: Oasisbr

Analysis of Bm86 conserved epitopes: is a global vaccine against Cattle Tick Rh...

Blecha,Isabella Maiumi Zaidan; Csordas,Bárbara Guimarães; Aguirre,André de Abreu Rangel; Cunha,Rodrigo Casquero; Garcia,Marcos Valério; Andreotti,Renato

Abstract The cattle tick Rhipicephalus microplus causes significant economic losses in agribusiness. Control of this tick is achieved mainly through the application of chemical acaricides, often resulting in contamination of animal food products and of the environment. Another major concern associated with acaricide use is the increasing reports of resistance of this tick vector against the active ingredients o...

Date: 2018   |   Origin: Oasisbr

Evaluation of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) resistance ...

Higa,Leandro de Oliveira Souza; Garcia,Marcos Valério; Barros,Jacqueline Cavalcante; Koller,Wilson Werner; Andreotti,Renato

Abstract The Rhipicephalus (Boophilus) microplus tick is responsible for considerable economic losses in Brazil, causing leather damage, weight loss and reduced milk production in cattle and results in the transmission of pathogens. Currently, the main method for controlling this tick is using acaricides, but their indiscriminate use is one of the major causes of resistance dissemination. In this study, the adu...

Date: 2016   |   Origin: Oasisbr

New insights from molecular characterization of the tick Rhipicephalus (Boophil...

Csordas,Bárbara Guimarães; Garcia,Marcos Valério; Cunha,Rodrigo Casquero; Giachetto,Poliana Fernanda; Blecha,Isabella Maiumi Zaidan; Andreotti,Renato

Abstract The Rhipicephalus (Boophilus) microplus complex currently consists of five taxa, namely R. australis, R. annulatus, R. (B.) microplus clade A sensu, R. microplus clade B sensu, and R. (B.) microplus clade C sensu. Mitochondrial DNA-based methods help taxonomists when they are facing the morpho-taxonomic problem of distinguishing members of the R. (B.) microplus complex. The purpose of this study was to...

Date: 2016   |   Origin: Oasisbr

Evaluation of arboviruses of public health interest in free-living non-human pr...

Rocha,Tatiana Carneiro da; Batista,Paulo Mira; Andreotti,Renato; Bona,Ana Caroline Dalla; Silva,Mário Antônio Navarro da; Lange,Rogério

INTRODUCTION: The aim of the present study was to evaluate the presence of arboviruses from the Flavivirus genus in asymptomatic free-living non-human primates (NHPs) living in close contact with humans and vectors in the States of Paraná and Mato Grosso do Sul, Brazil. METHODS: NHP sera samples (total n = 80, Alouatta spp. n = 07, Callithrix spp. n = 29 and Sapajus spp. n = 44) were screened for the presence o...

Date: 2015   |   Origin: Oasisbr

Neospora caninum and Toxoplasma gondii serodiagnosis in human immunodeficiency ...

Oshiro,Leandra Marla; Motta-Castro,Ana Rita Coimbra; Freitas,Solange Zacalusni; Cunha,Rodrigo Casquero; Dittrich,Rosangela Locatelli

ABSTRACTINTRODUCTION:Neospora caninum and Toxoplasma gondii belong to the Sarcocystidae family, and both have one definitive and various intermediary hosts. Owing to their weak immune systems, immunocompromised persons might be prone to opportunistic infections. The aim of this study was to investigate the presence of anti- N. caninum and anti- T. gondii antibodies in immunocompromised individuals.METHODS:This ...

Date: 2015   |   Origin: Oasisbr

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