14 documents found, page 1 of 2
Gene transcripts responsive to drought stress identified in Citrus macrophylla ...
da Silva, Melina; Pinto, Patricia IS; Guerra, Rui Manuel Farinha das Neves; Duarte, Amilcar; Power, Deborah; Marques, N T.Citrus macrophylla Wester (CM) has valuable agronomic characteristics such as the ability to grow in saline soils, although with low tolerance to prolonged drought stress (DS). To understand the mechanisms that characterize CM response to water scarcity, this study compared transcriptome profile changes in CM stem tissue when exposed to DS and identified a total of 2745 differentially expressed transcripts (DET...
HLB e a psila-africana-dos citrinos: um novo problema e novas soluções
Magalhães, Tomás; Duarte, Beatriz; Poeira, Rita; Neto, Luís; Marques, N T.; Duarte, AmilcarOs citrinos são uma cultura importante na Península Ibérica, fazendo parte da dieta mediterrânica e contribuindo para a economia dos dois países. Atualmente a citricultura desta região está ameaçada por uma doença devastadora, o huanglongbing, uma vez que a psila-africana-dos-citrinos, vetor da bactéria que causa esta doença, já se encontra em quase toda a região costeira de Portugal e também no norte de Espanh...
Trichome density in relation to volatiles emission and 1,8-Cineole synthase gen...
Marques, N T.; Filipe, Alexandra; Barroso, Jose; Trindade, Helena; Power, Deborah; Figueiredo, Ana Cristina; Pinto, Patricia IS1,8-Cineole is the main volatile produced by Thymus albicans Hoffmanns. & Link 1,8-cineole chemotype. To understand the contribution of distinct plant organs to the high 1,8-cineole production, trichome morphology and density, as well as emitted volatiles and transcriptional expression of the 1,8-cineole synthase (CIN) gene were determined separately for T. albicans leaves, bracts, calyx, corolla and infloresce...
Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses acc...
Varanda, C. M.; Materatski, P.; Campos, M. D.; Clara, M. I.; Marques, N T.; Nolasco, Gustavo; Felix, M. R.Previous extensive field surveys in olive orchards have revealed high levels of Olive mild mosaic virus (OMMV) and Olive latent virus 1 (OLV-1), frequently appearing in mixed infections. These viruses belong to genus Alphanecrovirus and their RNA dependent RNA polymerase (RdRp),as well as their p6 and p8 amino acid sequences share over 87% identity.
Molecular cloning and functional characterization of a monoterpene synthase iso...
Filipe, Alexandra; Cardoso, João; Miguel, Maria Graca; Anjos, Liliana; Trindade, Helena; Figueiredo, Ana Cristina; Barroso, Jose; Power, DeborahThe essential oil of Thymus albicans Hoffmanns. & Link, a native shrub from the Iberian Peninsula, is mainly composed of monoterpenes. In this study, a 1,8-cineole synthase was isolated from the 1,8-cineole chemotype. A partial sequence that lacked the complete plastid transit peptide but contained an extended C-terminal when compared to other related terpene synthases was generated by PCR and Rapid Amplificati...
Identification of asymptomatic plants infected with Citrus tristeza virus from a...
Afonso, Andreia; Guerra, Rui Manuel Farinha das Neves; Cavaco, A. M.; Pinto, Patricia IS; Andrade, André; Duarte, Amílcar; Power, Deborah; Marques, N T.Citrus tristeza virus (CTV) affects citrus crops with differing severity, depending on the viral strain, the citrus cultivar and the scion/rootstock combinations. In this study we address the problem of identifying asymptomatic infected plants using reflectance spectra of the leaves in the visible/near infrared region. Sixteen young citrus plants (8 Citrus x clementina hort. ex Tanaka ‘Fina’ and 8 Citrus sinens...
Combined expression of p20 and p23 proteins from Citrus tristeza virus show enh...
Costa, Angela A.; Martins, Tatiana R.; Marques, N T.; Nolasco, GustavoViruses developed a strategy to counter-defence the posttranscriptional gene silencing mechanism (PTGS) based on the activity of silencing suppressor proteins. Citrus tristeza virus (CTV), a member of the genus Closterovirus, has two suppressor proteins (p20 and p23) that target the local RNA silencing response of the host. In GFP transient co-expression assays performed on Nicotiana benthamiana 16C plants, loc...
Citrus tristeza virus p23 may suppress systemic silencing but is not related to...
Costa, A.; Marques, N T.; Nolasco, GustavoCitrus tristeza virus (CTV) the causal agent of the most devastating viral disease of citrus, has evolved three silencing suppressor proteins namely p20, p23 and p25 protein, previously reported for the monophyletic isolate T36. In this study the p23 suppressing activity from isolates of each of seven phylogenetic groups recognized for CTV was characterized in Nicotiana benthamiana 16C plants expressing GFP. Da...
Comparing p20's RNA silencing suppressing activity among five phylogenetic grou...
Marques, N T.; Costa, A. A.; Lopes, D.; Silva, Goncalo; Nolasco, GustavoThe p20 protein encoded by the Citrus Tristeza Virus (CTV) was previously identified as a RNA silencing suppressor. In this study, we analyzed the p20's suppressing activity from five phylogenetic groups of CTV, using the co-infiltration assay of Green fluorescence protein (GFP) gene and the suppressor gene in 16C line Nicotiana benthamiana plants. Green fluorescence, GFP mRNA relative levels and GFP specific s...
Identification of an RNA silencing suppressor encoded by Grapevine leafroll-ass...
Gouveia, P.; Dandlen, S. A.; Costa, A.; Marques, N T.; Nolasco, GustavoGLRaV-3, a member of the Closteroviridae family and type member of the genus Ampelovirus, is involved in the grapevine leafroll disease. Until now no RNA silencing suppressor has been found among viruses of this genus, contrary to what happens with a large number of other viral genera. In the sister genus Closterovirus, RNA silencing suppressors are present in the 3' end of the genome and have molecular weights...