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Polyhydroxyalkanoates production from a waste-derived feedstock driven by the r...

Guarda, Eliana C.; Amorim, Catarina L.; Pasculli, Gabriele; Castro, Paula M. L.; Galinha, Claudia F.; Duque, Anouk F.; Reis, Maria A. M.

In the last years, the production of plastics at large-scale has been an issue of great concern due to their environmental and health impacts. Biobased and biodegradable plastics, such as polyhydroxyalkanoates (PHA), have emerged as a possible more ecological and sustainable alternative to those conventional plastics. PHA can be produced by mixed microbial cultures (MMC) from waste-based feedstocks. This work a...


Can biomass mastication assist the downstreaming of polyhydroxyalkanoates produ...

Souza, Hiléia Karla Silva; Matos, Mariana; Reis, Maria A. M.; Covas, J. A.; Hilliou, L.

Polyhydroxyalkanoates (PHAs) are natural polyesters which biodegrade in soils and oceans but have more than double the cost of comparable oil-based polymers. PHA downstreaming from its biomass represents 50% of its overall cost. Here, in an attempt to assist downstreaming, mastication of wet biomasses is tested as a new mechanical continuous biomass pretreatment with potential for industrial upscaling. Downstre...


Development of a layered bacterial nanocellulose-PHBV composite for food packaging

Silva, Francisco Almeida Garrett Soares; Matos, Mariana; Dourado, Fernando; Reis, Maria A. M.; Branco, Pedro C.; Poças, Fátima; Gama, F. M.

BACKGROUND Most of the current materials used in food packaging are synthetic and non-degradable, raising environmental issues derived from the accumulation of plastics in landfills/waterways. The food industry increasingly needs eco-friendly sustainable materials that meet the food packaging requirements. Bacterial nanocellulose (BNC), a biopolymer obtained by fermentation, offers very good mechanical properti...


Acidogenic fermentation of brewers’ spent grain monitored through two-dimension...

Guarda, Eliana C.; Costa, Eunice; Gil, Cátia; Amorim, Catarina L.; Galinha, Claudia F.; Duque, Anouk F.; Castro, Paula M. L.; Reis, Maria A. M.

Biological systems are commonly controlled and monitored through offline and time-consuming tools, which often impairs an effective and real-time response to counteract system disturbances. The feasibility of using two-dimensional (2D) fluorescence spectroscopy as a non-invasive, non-destructive, and real-time procedure to monitor the acidogenic fermentation of brewer’s spent grain (BSG) in a granular sludge re...


A novel sustainable PHA downstream method

Mondal, Suchintan; Syed, Usman T.; Gil, Cátia; Hilliou, L.; Duque, Anouk F.; Reis, Maria A. M.; Brazinha, Carla

The pernicious impact of plastic originating from petroleum-based sources has been an increasing threat to our environment. Amongst several strategies tested and implied to manage these plastic wastes, one promising alternative is switching to biodegradable polymers. Polyhydroxyalkanoates (PHAs) are a class of microbial polyesters that are biodegradable, biocompatible and an attractive alternative for future pa...


The microbiome of anaerobic granules during volatile fatty acid production is s...

Guarda, Eliana C.; Costa, Eunice; Gil, Cátia; Galinha, Cláudia F.; Amorim, Catarina L.; Duque, Anouk F.; Castro, Paula M. L.; Reis, Maria A. M.


Polyhydroxyalkanoates from industrial cheese whey: production and characterizat...

Carvalheira, Mónica; Hilliou, Loic; Oliveira, Catarina S. S.; Guarda, Eliana C.; Reis, Maria A. M.

The composition of polyhydroxyalkanoate (PHA) monomers affects the properties and final applications of PHA polymers. This study focused on the feasibility of producing tailored PHA differing in hydroxyvalerate (HV) content, through manipulation of the acidogenic fermented stream composition, and on the characterization of PHA properties to determine the best composition for melt processing. Cheese whey was use...


How does knowledge on the microbiome can help advancing the valorization of bre...

Guarda, Eliana C.; Costa, Eunice; Galinha, Cláudia F.; Gil, Cátia; Amorim, Catarina L.; Duque, Anouk F.; Castro, Paula; Reis, Maria A. M.


Fluctuations of the pH shape the microbiome of anaerobic granules during volati...

Guarda, Eliana C.; Costa, Eunice; Gil, Cátia; Amorim, Catarina L.; Galinha, Cláudia F.; Duque, Anouk F.; Castro, Paula M. L.; Reis, Maria A. M.


Valorization of brewery waste through polyhydroxyalkanoates production supporte...

Carvalheira, Mónica; Amorim, Catarina L.; Oliveira, Ana Catarina; Guarda, Eliana C.; Costa, Eunice; Teixeira, Margarida Ribau; Castro, Paula M. L.

Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. Thi...


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