53 documents found, page 1 of 6
Imobilização de lipase de aspergillus niger C em nanoparticulas magnéticas de h...
Santos, T. C.; Viana, J. S; Terzi, Selma da Costa; Paris, Elaine Cristina; Cabral, Lourdes Maria Corrêa; Brígida, Ana Iraidy SantaO presente trabalho teve por objetivo desenvolver um método para imobilizar lipase de Aspergillus niger C em nanopartículas magnéticas de hidroxiapatita e óxido de ferro (1:1). E a caracterização do mesmo também foi realizada. A imobilização foi efetuada por meio de processo adsortivo. A cinética de adsorção mostrou equilíbrio a partir de 4h de imobilização para a atividade do derivado, com rendimento máximo em...
Dendrimers: breaking the paradigm of current musculoskeletal autoimmune therapies
Rodrigues, Daniel Barreira; Oliveira, Joaquim M.; Santos, T. C.; Reis, R. L.The advances achieved by cell-based therapies to treat autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS), despite promising, are still insufficient for the current demands. RA and MS therapeutic approaches follow world guidelines to use disease modifying drugs and biological agents that, regardless of some good results in clinical outcomes, are well known for several systemic sec...
Simultaneous determination of standardized ileal digestible tryptophan and lysi...
Pasquetti, T. J.; Pozza, P. C.; Moreira, I.; Santos, T. C.; Diaz-Huepa, L. M.; Castilha, L. D.; Perondi, D. [UNESP]; Carvalho, P. L. O.; Kim, S. W.Made available in DSpace on 2018-11-26T16:18:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-11-01; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); The digestible amino acid (AA) determination of ingredients, or experimental diets, are essential in obtaining the real levels of AA that are being studied, as well ...
Stem cell-containing hyaluronic acid-based spongy hydrogels for integrated diab...
Silva, Lucília Pereira; Santos, T. C.; Rodrigues, Daniel Barreira; Pirraco, Rogério P.; Cerqueira, Mariana Teixeira; Reis, R. L.; Correlo, V. M.The detailed pathophysiology of diabetic foot ulcers is yet to be established and improved treatments are still required. We propose a strategy that directs inflammation, neovascularization, and neoinnervation of diabetic wounds. Aiming to potentiate a relevant secretome for nerve regeneration, stem cells were precultured in hyaluronic acid-based spongy hydrogels under neurogenic/standard media before transplan...
In vivo osteogenic differentiation of stem cells inside compartmentalized capsu...
Correia, C.; Santos, T. C.; Pirraco, R. P.; Cerqueira, M. T.; Marques, A. P.; Reis, R. L.; Mano, J. F.Capsules coated with polyelectrolytes and co-encapsulating adipose stem (ASCs) and endothelial (ECs) cells with surface modified microparticles are developed. Microparticles and cells are freely dispersed in a liquified core, responsible to maximize the diffusion of essential molecules and allowing the geometrical freedom for the autonomous three-dimensional (3D) organization of cells. While the membrane wraps ...
Eumelanin-releasing spongy-like hydrogels for skin re-epithelialization purposes
Silva, Lucília Pereira; Oliveira, Sílvia; Pirraco, Rogério P.; Santos, T. C.; Reis, R. L.; Marques, A. P.; Correlo, V. M.Melanin function in the skin has been associated with pigmentation but other properties such as electrical conductance, photoprotection, and antioxidant and antimicrobial activity have also been recognized. Nonetheless, the use of melanin in a skin wound healing context has never been considered. In this sense, eumelanin particles with a typical round and nano-sized morphology and electrical conductivity of 2.0...
Can host reaction animal models be used to predict and modulate skin regeneration?
Santos, T. C.; Reis, R. L.; Marques, A. P.The study of host reactions in the biomedical and tissue engineering (TE) fields is a key issue but somehow set aside where TE constructs are concerned. Every day new biomaterials and TE constructs are being developed and presented to the scientific community. The combination of cells and biomolecules with scaffolding materials, as TE constructs, make the isolation and the understanding of the effect of each on...
Cell sheet engineering using the stromal vascular fraction of adipose tissue as...
Costa, M.; Cerqueira, Mariana Teixeira; Santos, T. C.; Marques, Belém Sampaio; Ludovico, Paula; Marques, A. P.; Pirraco, Rogério P.; Reis, R. L.Current vascularization strategies for Tissue Engineering constructs, in particular cell sheet-based, are limited by time-consuming and expensive endothelial cell isolation and/or by the complexity of using extrinsic growth factors. Herein, we propose an alternative strategy using angiogenic cell sheets (CS) obtained from the stromal vascular fraction (SVF) of adipose tissue that can be incorporated into more c...
Natural melanin promotes the differentiation of an early epidermal cell fractio...
Silva, Lucília Pereira; Oliveira, Sílvia; Lago, M. E. L.; Cerqueira, Mariana Teixeira; Pirraco, Rogério P.; Santos, T. C.; Reis, R. L.; Correlo, V. M.The aim of the study was to determine whether melanin extracted from Sepia officinalis , common cuttlefish ink, affects the phenotype of an interfollicular keratinocyte cell fraction, and therefore propose a strategy to enhance wound re-epithelialization that also provides protection against ROS. To demonstrate the properties of the melanin, rapidly adherent human keratinocytes (KCs, passage<2, n=3) were expose...
Neovascularization induced by the hyaluronic acid-based spongy-like hydrogels d...
Silva, Lucília Pereira; Pirraco, Rogério P.; Santos, T. C.; Novoa-Carballal, R.; Cerqueira, Mariana Teixeira; Reis, R. L.; Correlo, V. M.Neovascularization has been a major challenge in many tissue regeneration strategies. Hyaluronic acid (HA) of 3â 25 disaccharides is known to be angiogenic due to its interaction with endothelial cell receptors. This effect has been explored with HA-based structures but a transitory response is observed due to HA burst biodegradation. Herein we developed gellan gum (GG)-HA spongy-like hydrogels from semi-interp...