11 documents found, page 1 of 2
Cytokine-based insights into bloodstream infections and bacterial gram typing i...
Araújo, Rúben Alexandre Dinis; Ramalhete, Luís; Von Rekowski, Cristiana; Henrique Fonseca, Tiago Alexandre; Calado, Cecília; Bento, LuísTimely and accurate identification of bloodstream infections (BSIs) in intensive care unit (ICU) patients remains a key challenge, particularly in COVID-19 settings, where immune dysregulation can obscure early clinical signs. Methods: Cytokine profiling was evaluated to discriminate between ICU patients with and without BSIs, and, among those with confirmed BSIs, to further stratify bacterial infections by Gra...
Integration of FTIR spectroscopy and machine learning for kidney allograft reje...
Ramalhete, Luís; Araújo, Rúben Alexandre Dinis; Bigotte Vieira, Miguel; Vigia, Emanuel; Aires, Inês; Ferreira, Aníbal; Calado, CecíliaKidney transplantation is a life-saving treatment for end-stage kidney disease, but allograft rejection remains a critical challenge, requiring accurate and timely diagnosis. The study aims to evaluate the integration of Fourier Transform Infrared (FTIR) spectroscopy and machine learning algorithms as a minimally invasive method to detect kidney allograft rejection and differentiate between T Cell-Mediated Reje...
Survivability prediction based on the serum molecular fingerprint in critically...
Correia, Inês; Araújo, Rúben Alexandre Dinis; Henrique Fonseca, Tiago Alexandre; Von Rekowski, Cristiana; Bento, Luís; Calado, CecíliaIt is relevant to discover biomarkers enabling to predict critically ill patients’ survival. This study focused on 45 patients, from which 22 deceased and 23 were discharged from an Intensive Care Unit (ICU). It was considered the serum molecular fingerprint, as acquired by Fourier Transform Infra-Red (FTIR) spectroscopy, obtained 3 days before the patients discharged or death at the ICU. It was possible to obt...
Infection biomarkers at intensive care units
Araújo, Rúben Alexandre Dinis; Ramalhete, Luís; Henrique Fonseca, Tiago Alexandre; Von Rekowski, Cristiana; Bento, Luís; Calado, CecíliaIt is relevant to discover infection biomarkers, especially for critically ill patients in intensive care units (ICU), as these patients often present non-infectious inflammatory processes that obscure typical infectious markers. This study focused on 20 ICU patients, half of whom had acquired bacterial blood infections (bacteremia). Due to the significance of inflammatory processes in these patients, it was ev...
Trends in COVID-19 patient characteristics and mortality throughout the pandemi...
Von Rekowski, Cristiana; Pinto, Iola; Henrique Fonseca, Tiago Alexandre; Araújo, Rúben Alexandre Dinis; Ferreira, Artur; Calado, Cecília; Bento, LuísAs SARS-CoV-2 continues to circulate globally and new variants emerge, it remains relevant to gather data on the affected patients’ clinical characteristics and outcomes to understand how individual factors and public health measures affect prognosis. Thus, we analyzed data of 870 ICU patients admitted for COVID-19 across two distinct phases of the pandemic: before and after the introduction of immunization. Ex...
Predicting critically ill patients outcome in the ICU using UHPLC-HRMS data
Henrique Fonseca, Tiago Alexandre; Von Rekowski, Cristiana; Araújo, Rúben Alexandre Dinis; Oliveira, Maria Conceição; Bento, Luís; Justino, GonçaloThe available scores to predict patients’ outcomes in specific settings generally present low sensitivities and specificities when applied to intensive care units’ (ICUs) populations. Advancements in analytical techniques, notably Ultra-High Performance Liquid Chromatography- Mass Spectrometry (UHPLCHRMS) transformed biomarker identification, enabling a comprehensive profiling of biofluids, including serum. In ...
Comparison of the serum whole molecular composition with the serum metabolome t...
Correia, Inês; Henrique Fonseca, Tiago Alexandre; Pataco, Jéssica; Oliveira, Mafalda; Caldeira, Viviana; Domingues, N.; Von Rekowski, CristianaOmics Sciences serve as an essential tool to advance precision medicine. Since conventional omics sciences rely on laborious, complex and time-consuming analytical processes, this study evaluated whether the serum molecular fingerprint, captured by FTIR spectroscopy, could predict mortality risk in critically ill patients. Both the whole serum and the serum metabolome (i.e., serum after removal of macromolecule...
Early mortality prediction in intensive care unit patients based on serum metab...
Araújo, Rúben Alexandre Dinis; Ramalhete, Luís; Von Rekowski, Cristiana; Henrique Fonseca, Tiago Alexandre; Bento, Luís; Calado, CecíliaPredicting mortality in intensive care units (ICUs) is essential for timely interventions and efficient resource use, especially during pandemics like COVID-19, where high mortality persisted even after the state of emergency ended. Current mortality prediction methods remain limited, especially for critically ill ICU patients, due to their dynamic metabolic changes and heterogeneous pathophysiological processe...
Impact of Epigallocatechin-3-gallate (EGCG) on the molecular profile of plasma ...
Araújo, Rúben Alexandre DinisBackground and Goals: Epigallocatechin-3-gallate (EGCG) is the major catechin present in green tea and it is known to display diverse biological activities as antioxidation, antiinflammation, antiproliferation, antimicrobial, antiviral, among others. The present work aimed to evaluate the impact of consumption of EGCG on the molecular profile of human serum and plasma. Methods: The effect of the consumption of ...
Impact of Epigallocatechin-3-gallate (EGCG) on the molecular profile of plasma ...
Araújo, Rúben Alexandre DinisABSTRACT - Background and Goals: Epigallocatechin-3-gallate (EGCG) is the major catechin present in green tea and it is known to display diverse biological activities as antioxidation, antiinflammation, antiproliferation, antimicrobial, antiviral, among others. The present work aimed to evaluate the impact of consumption of EGCG on the molecular profile of human serum and plasma. Methods: The effect of the cons...