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Omega-3 and conjugated fatty acids impact on human microbiota modulation using ...

Salsinha, Ana Sofia; Araújo-Rodrigues, Helena; Dias, Cindy; Cima, André; Rodríguez-Alcalá, Luís Miguel; Relvas, João B.; Pintado, Manuela

Background& aims: Gut microbiota has been gaining increasing attention and itsimportant role in the maintenance of a general good health condition is alreadyestablished. The potential of gut microbiota modulation through diet is animportant research focus to be considered. Lipids, as omega-3 fatty acids, arewell known for their beneficial role on organs and corresponding diseases.However, their impact on gut mi...


Comparative analysis of polysaccharide and nutritional composition of biologica...

Araújo-Rodrigues, Helena; Amorim, Manuela; Freitas, Victor de; Relvas, João B.; Tavaria, Freni K.; Pintado, Manuela

This study chemically characterized three Pleurotus ostreatus fruiting bodies cultivated in the Iberian Peninsula under different conditions (biological and industrial), with emphasis on polysaccharide analysis. Comprehensive comparative data on cultivation-dependent nutritional variations will potentially improve their nutritional and therapeutic applications. Industrial mushrooms (POC and POA) contained signi...


Impact of gastrointestinal tract on mushroom biomass: deciphering their potenti...

Araújo-Rodrigues, Helena; Salsinha, Ana Sofia; Relvas, João Bettencourt; Tavaria, Freni K.; Pintado, Manuela E.

Resume: Prebiotic supplementation is an extensively used strategy for treating several disorders. It plays key roles in promoting gut microbiota balance. Besides nutritional value, mushrooms are a rich source of bioactive compounds (e.g., α-/β-glucans) with prebiotic potential. While most research studies focus on testing extracts, a positive synergistic effect among mushroom biomass (MB) macromolecules has bee...


The potential of acorn extract treatment on PUFAS oxidative stability: a case s...

Araújo-Rodrigues, Helena; Ribeiro, Tânia Bragança; Machado, Manuela; Pereira, Carlos D.; Pintado, Manuela E.

Fish byproducts are valuable sources of Ω-3 polyunsaturated fatty acids (PUFAs). Their valorization potentially alleviates pressure on this sector. This study uses a circular economy approach to investigate the oil fraction from sardine cooking wastewater (SCW). Analysis of its fatty acid (FA) profile revealed promising PUFA levels. However, PUFAs are highly susceptible to oxidation, prompting the exploration o...


The use of cecal samples from Wistar rats in in vitro fermentation to determine...

Salsinha, Ana Sofia; Cima, André; Araújo-Rodrigues, Helena; Viana, Sofia; Reis, Flávio; Coscueta, Ezequiel R.; Rodríguez-Alcalá, Luis Miguel

The influence of gut microbiota in the onset and development of several metabolic diseases, such as obesity and diabetes, has gained attention over the last few years. Indeed, diet plays an essential role in gut microbiota modulation. Western diet (WD), characterized by high-sugar and high-fat consumption, alters gut microbiome composition, diversity index, microbial relative levels, and functional pathways. Se...


An overview on mushroom polysaccharides: health-promoting properties, prebiotic...

Araújo-Rodrigues, Helena; Sousa, Ana Sofia; Relvas, João Bettencourt; Tavaria, Freni K.; Pintado, Manuela

Mushroom polysaccharides are recognized as “biological response modifiers”. Besides several bioactivities, a growing interest in their prebiotic potential has been raised due to the gut microbiota modulation potential. This review comprehensively summarizes mushroom polysaccharides' biological properties, structure-function relationship, and underlying mechanisms. It provides a recent overview of the key findin...


Solubilization and hydrolysis of porcine coagulated blood protein using sub-cri...

Marques, Bianca; Nunes, Rafaela; Araújo-Rodrigues, Helena; Pintado, Manuela; Pereira, Ricardo N.; Teixeira, José A.; Rocha, Cristina M. R.

Pork represents a major fraction of the meat consumed worldwide but only 30% of the blood generated in slaughterhouses is re-used as raw material for food and feed. Innovative technologies and efficient processing strategies capable of generating added-value products from it are now attracting attention. In this study, the hydrolysis of porcine coagulated blood using sub-critical solvent extraction was investig...


Impact of washing and freezing on nutritional composition, bioactive compounds,...

Marçal, Sara; Sousa, Sérgio; Araújo-Rodrigues, Helena; Silva, Inês V.; Campos, Débora A.; Pintado, Manuela

Mango peels are widely produced and highly perishable. Disinfectant washing and freezing are among the most used methods to preserve foods. However, their impact on products’ properties is conditioned by the foods’ features. This study evaluated for the first time the phytochemical composition, antioxidant activity, and microstructure of mango peels washed with peracetic acid (27 mg/mL for 19 min) and frozen at...


Influence of the gastrointestinal tract on mushroom biomass: understanding thei...

Araújo-Rodrigues, Helena; Maziero Fogarin, Henrique; Salsinha, Ana Sofia; de Carvalho, Nelson Mota; Relvas, João Bettencourt; Tavaria, Freni K.

Recent research data have suggested the high therapeutical potential of gut microbiota modulation in numerous diseases. Nowadays, fecal transplantation, probiotics and prebiotics supplementation are strategies extensively used in the treatment of several disorders. Prebiotic-rich foods play an important role in gut microbiota modulation. Beyond the rich nutritional value and various biological properties of mus...


Solubilization and hydrolysis of porcine coagulated blood protein using sub-cri...

Marques, Bianca; Nunes, Rafaela; Araújo-Rodrigues, Helena; Pintado, Manuela; Pereira, Ricardo N.; Teixeira, J. A.; Rocha, Cristina M.R.

Pork represents a major fraction of the meat consumed worldwide but only 30% of the blood generated in slaughterhouses is re-used as raw material for food and feed. Innovative technologies and efficient processing strategies capable of generating added-value products from it are now attracting attention. In this study, the hydrolysis of porcine coagulated blood using sub-critical solvent extraction was investig...


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