Extractive fermentation is an integrated process that uses aqueous biphasic systems to combine the production and primary purification procedures into a single step. With this concept, the main goal of this work was to design a bioprocess that integrates laccase production and purification using a genetically engineered yeast (Pichia pastoris), creating a competitive and sustainable process. Successful expressi...
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Based on the principles of green chemistry, enzymatic biocatalysis contributes to a more eco-friendly and sustainable industrial chemistry. Therefore, its wide use in industrial and biotechnological processes has increasingly become more evident. One promising application is the enzymatic polymerization of various compounds. Laccase, a multicopper oxidase with high catalytic efficiency and low substrate specifi...