Portugal’s architecture reflects a rich history influenced by Roman, Moorish, Neoclassical and Romantic styles, with the 20th century marked by Art Deco and colonial influences. Regional styles vary, with white-painted buildings commonly experiencing color changes due to biofilm formation. Visually striking are the red-colored biological patinascommon in the coastal areas. A survey of 120 historical buildings a...
Caves, with distinctive features and specialized ecosystems, harbour microorganisms that have adapted metabolically to survive in seemingly harsh conditions with limited essential elements. Despite the potential threat of biodeterioration to subterranean heritage due to microbial proliferation, caves serve as vital reservoirs of bioactive compounds produced by subsurface-adapted microorganisms. These microorgan...
The Eugénio de Almeida Foundation’s Casa de Fresco is a historical monument of valuable historic–artistic significance, which currently reveals an assortment of biofilms due to the proliferation of microorganisms in the stone and rocaille elements. The biodeterioration in this area was studied as part of the Conservation and Restoration Project. We effectively characterized the local microbial community using m...
Paenibacillus, rod-saped gram-positive endospores forming aerobic or facultative anaerobic bacteria, colonize diverse ecosystems and are involved in the biodegradation of cultural heritage assets. Biodeteriogenic microorganisms can be easily detected/identified by ribonucleic acid- fluorescent in situ hybridization RNA-FISH with specific probes. In this work, probes designed in silico were analyzed to calculate...
Mastitis in ewes is responsible for massive economic losses. Antibiotics are routinely used for mastitis control but its excessive use leads to development of antibiotic resistance with deleterious outcomes both for animal and public health. Essential oils (EOs) show antibacterial proprieties and no resistance has been reported after prolonged exposure; however their efficacy depends on their chemical compositi...
To distinguish Rhodotorula sp., from other microorganisms that produce the same type of alterations on CH materials, proper identification methods must be applied. RNA-fluorescence and in situ hybridization (RNA-FISH) has the potential to specifically identify the target microorganism of interest in complex microbial communities (it is based on hybridization of fluorescently-labeled oligonucleotide probes targe...