Fluorescence microscopy is a highly sensitive imaging technique used in various scientific fields such as cellular biology, environmental sciences, medicine, and pharmacy. It offers the advantage of using multiple fluorescent labels to visualize different biomolecules and generate multicolored images for identifying specific components within complex biomolecular structures and studying their interactions. Thes...
Fluorescent labels, commonly used in highly sensitive analytical techniques for detecting and tracking biomolecules in critical fields like cellular biology, medicine, medicinal chemistry, and environmental science, are currently too expensive for routine use in standard applications, with most exhibiting small Stokes shifts. This limitation underscores the potential of 4-diethylaminobenzaldehyde derivatives as...
Portugal’s architecture reflects a rich history influenced by Roman, Moorish, Neoclassical and Romantic styles, with the 20th century marked by Art Deco and colonial influences. Regional styles vary, with white-painted buildings commonly experiencing color changes due to biofilm formation. Visually striking are the red-colored biological patinascommon in the coastal areas. A survey of 120 historical buildings a...
Currently, fluorescence microscopy is one of the highly sensitive imaging techniques that allow visualize, detect, and track biomolecules in analytical studies in many important scientific areas as cellular biology, environmental sciences, medicine, pharmacy, among others. One of the great advantages of fluorescence microscopy is the possibility to use several fluorescent labels to detect different biomolecules...
Cultural heritage assets are being continuously submitted to physical, chemical and biological degradation. Biodegradation is the major factor for their deterioration, with microorganisms being of critical importance due to their metabolism and proliferation. In this context, it is frequently necessary identify and locate individual microorganisms and detect small amounts of biomolecules, as proteins, to know, ...
Biodeterioration of Cultural Heritage is the consequence of interactions between living organisms, material support and environmental conditions. In stone materials, deterioration by fungi is mainly the result of mechanical, chemical, and physical processes, consisting essentially of penetration of hyphae, production of fruiting bodies, production and release of destructive extracellular organic acids, enzymes,...
Pristine environments can be defined as places with limited or no connections to anthropogenic activities [1], for example, karstic and marine caves, important landmarks of Natural and Cultural Heritage. Usually, these environments are exposed to extreme factors such as temperature, salinity, osmolarity, UV radiation, pressure, or pH, with values close to the limit of life. In these extreme environments, living...
Pristine environments can be defined as places with limited or no connections to anthropogenic activities [1], for example, karstic and marine caves, important landmarks of Natural and Cultural Heritage. Usually, these environments are exposed to extreme factors such as temperature, salinity, osmolarity, UV radiation, pressure, or pH, with values close to the limit of life. In these extreme environments, living...
Caso de estudo da biodeterioração da Casa de Fresco pertencentes ao jardim da Fundação Eugénio de Almeida em Évora (Portugal). A investigação foi realizada com os principais objetivos de: i) caracterização dos materiais utilizados; ii) avaliação e caracterização da comunidade microbiana através de técnicas metagenómicas filogenéticas e iii) investigação de biocidas comerciais, de forma a promover a salvaguarda ...
Aromatic plants are appreciated as food flavors, being preserved and consumed by the local population in daily diets, and in Mediterranean Diet. Essential oils (EOs), complex mixtures rich in terpene compounds, have shown several biological properties, mainly as antimicrobials and antioxidants. Alentejo, S.W. of Portugal, is very rich in flavoring autochthones aromatic plants. Therefore, it is important to eval...