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Straightforward purification method for the determination of the activity of gl...

González-Ceballos, Lara; Carlos Guirado-Moreno, Jose; Utzeri, Gianluca; Miguel García, José; Fernández-Muiño, Miguel A.; Osés, Sandra M.

Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and p...


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