10 documents found, page 1 of 1
Proteomics insights into the responses of Saccharomyces cerevisiae during mixed...
Peng, Chuantao; Andersen, Birgit; Arshid, Samina; Larsen, Martin R.; Albergaria, Helena; Lametsch, Rene; Arneborg, NilsABSTRACT: The response of Saccharomyces cerevisiae to cocultivation with Lachancea thermotolerans during alcoholic fermentations has been investigated using tandem mass tag (TMT)-based proteomics. At two key time-points, S. cerevisiae was sorted from single S. cerevisiae fermentations and from mixed fermentations using flow cytometry sorting. Results showed that the purity of sorted S. cerevisiae was above 96% ...
Effect of GAPDH-derived antimicrobial peptides on sensitive yeasts cells: membr...
Branco, Patricia; Albergaria, Helena; Arneborg, Nils; Prista, CatarinaABSTRACT: Saccharomyces cerevisiae secretes antimicrobial peptides (AMPs) derived from glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which induce the death of several non-Saccharomyces yeasts. Previously, we demonstrated that the naturally secreted GAPDH-derived AMPs (i.e. saccharomycin) caused a loss of culturability and decreased the intracellular pH (pHi) of Hanseniaspora guilliermondii cells. In this st...
Cell-to-cell contact and antimicrobial peptides play a combined role in the dea...
Kemsawasd, Varongsiri; Branco, Patrícia; Almeida, Maria Gabriela; Caldeira, Jorge; Albergaria, Helena Albergaria; Arneborg, NilsThe roles of cell-to-cell contact and antimicrobial peptides in the early death of Lachanchea thermotolerans CBS2803 during anaerobic, mixed-culture fermentations with Saccharomyces cerevisiae S101 were investigated using a commercially available, double-compartment fermentation system separated by cellulose membranes with different pore sizes, i.e. 1000 kDa for mixed- and single-culture fermentations, and 1000...
Identification of novel GAPDH-derived antimicrobial peptides secreted by Saccha...
Branco, Patrícia; Francisco, Diana; Chambon, Christophe; Hébraud, Michel; Arneborg, Nils; Almeida, Maria Gabriela; Caldeira, Jorge; Albergaria, HelenaSaccharomyces cerevisiae plays a primordial role in alcoholic fermentation and has a vast worldwide application in the production of fuel-ethanol, food and beverages. The dominance of S. cerevisiae over other microbial species during alcoholic fermentations has been traditionally ascribed to its higher ethanol tolerance. However, recent studies suggested that other phenomena, such as microbial interactions medi...
Identification of novel GAPDH-derived antimicrobial peptides secreted by Saccha...
Branco, Patricia; Francisco, Diana; Chambon, Christophe; Hébraud, Michel; Arneborg, Nils; Gabriela Almeida, M.; Caldeira, Jorge; Albergaria, HelenaSaccharomyces cerevisiae plays a primordial role in alcoholic fermentation and has a vastworldwide application in the production of fuel-ethanol, food and beverages. The dominance of S. cerevisiae over other microbial species during alcoholic fermentations has been traditionally ascribed to its higher ethanol tolerance. However, recent studies suggested that other phenomena, such as microbial interactions media...
Interactions between Saccharomyces cerevisiae and Hanseniaspora guilliermondii:...
Branco, Patricia; Kemsawasd, Varongsiri; Arneborg, Nils; Albergaria, HelenaSeveral studies have shown that the early death of non-Saccharomyces during wine fermentations are due to yeast-yeast interactions induced by Saccharomyces cerevisiae (Sc) through different mechanisms such as growth arrest mediated by a cell-cell contact mechanism (Nissen et al. 2003) and death mediated by killer-like toxins (Pérez-Nevado et al 2006; Albergaria et al. 2010). Besides, previous work also showed t...
Dominance of Saccharomyces cerevisiae in wine fermentations: secretion of antim...
Albergaria, Helena; Branco, Patricia; Francisco, Diana; Coutinho, Rute; Monteiro, Margarida; Malfeito-Ferreira, Manuel; Arneborg, NilsIn present work we investigated the antagonistic effect by S. cerevisiae against several wine-related microbial species vis-à-vis the secretion of antimicrobial peptides (AMPs).
Antimicrobial peptides from Saccharomyces cerevisiae induce physiological chang...
Branco, Patricia; Albergaria, Helena; Arneborg, NilsSaccharomyces cerevisiae secretes antimicrobial peptides (AMPs) during alcoholic fermentation that are active against other wine-related yeasts (e.g. Hanseniaspora guilliermondii) (Albergaria et al., 2010) and bacteria (e.g. Oenococcus oeni) (Osborne and Edwards, 2007). In the present study we assessed the physiological changes induced by those AMPs on sensitive yeast cells of Hanseniaspora guilliermondii, name...
Interactions between Saccharomyces cerevisiae and Hanseniaspora guilliermondii:...
Branco, Patricia; Kemsawasd, Varongsiri; Arneborg, Nils; Albergaria, HelenaSeveral studies have shown that the early death of non-Saccharomyces during wine fermentations are due to yeast-yeast interactions induced by Saccharomyces cerevisiae (Sc) through different mechanisms such as growth arrest mediated by a cell-cell contact mechanism (Nissen et al. 2003) and death mediated by killer-like toxins (Pérez-Nevado et al 2006; Albergaria et al. 2010). Besides, previous work also showed t...
Dominance of Saccharomyces cerevisiae in wine fermentations: secretion of antim...
Albergaria, Helena; Branco, Patricia; Francisco, Diana; Coutinho, Rute; Monteiro, Margarida; Malfeito-Ferreira, Manuel; Arneborg, NilsIn present work we investigated the antagonistic effect by S. cerevisiae against several wine-related microbial species vis-à-vis the secretion of antimicrobial peptides (AMPs).