The species in Amanita ser. Amidella (E.-J.Gilbert) Neville & Poumarat form a well-defined clade, but some taxa remain difficult to discriminate. In particular, the concept of Amanita valens (E.-J.Gilbert) Bertault remains controversial. To understand the phylogenetic placement of a set of collections from southern Portugal with a novel nrDNA barcode, we have obtained nrDNA sequences for previously unassessed t...