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Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cyto...
Sesso, Antonio; Yamashiro-Kanashiro, Edite Hatsumi; Arruda, Liã Bárbara; Kawakami, Joyce; Higuchi, Maria de Lourdes; Orii, Noemia MieA study with transmission electron microscopy of mycoplasma-contaminated HeLa cells using five cell donors referred to as donors A, B, C, D and E, observations are herein presented. Experiments performed with cells from donors B, C and D, revealed the presence of Mycoplasma hyorhinis after PCR and sequencing experiments. Bacteria probably originated from a cytoplasm with compacted tiny granular particles replac...
DETERMINATION OF VIRAL TROPISM BY GENOTYPING AND PHENOTYPING ASSAYS IN BRAZILIA...
Arruda, Liã Bárbara; Araújo, Marilia Ladeira de; Martinez, Maira Luccia; Gonsalez, Claudio Roberto; Duarte, Alberto José da Silva; Coakley, EoinThe clinical application of CCR5 antagonists involves first determining the coreceptor usage by the infecting viral strain. Bioinformatics programs that predict coreceptor usage could provide an alternative method to screen candidates for treatment with CCR5 antagonists, particularly in countries with limited financial resources. Thus, the present study aims to identify the best approach using bioinformatics to...
TESTING A SUBTYPE-SPECIFIC GP41 AMPLIFICATION METHOD FOR GENOTYPING INDIVIDUALS...
Arruda, Liã Bárbara; Weber, Laura I.; Santos, Marisa dos; Kawakubo, Edson M.; Martínez, Ana Maria B.The method used by YAGYU et al. for the subtype-specific polymerase chain reaction (PCR) amplification of the gp41 transmembrane region of the human immunodeficiency virus type-1 (HIV-1) env gene, was tested. HIV-1 proviral DNA from 100 infected individuals in Itajaí, South Brazil was used to analyze this method. Seventy individuals were determined according to this method as having PCR products at the expected...
Prevalence of Mycoplasma genitalium among HIV-infected men in São Paulo city de...
Arruda, Liã Bárbara