10 documents found, page 1 of 1
Venom of the giant ant Dinoponera quadriceps attenuates inflammatory pain in mo...
Assreuy, Ana Maria Sampaio; Adjafre, Beatriz Lima; Sousa, Évilla Rayanne Oliveira; Alves, Ana Paula Negreiros Nunes; Pires, Alana de FreitasArthropod venoms are potential sources of bioactive substances, providing tools for the validation of popular use and new drugs design. Ants belonging to the genus Dinoponera are used in the folk medicine to treat inflammatory conditions. It was previously demonstrated that the venom of the giant ant Dinoponera quadriceps (DqV), containing a mixture of polypeptides, elicit antinociceptive effect in mi...
Purification and primary structure determination of a galactose-specific lectin...
Silva, Helton Colares; Nagano, C. S.; Souza, Luis A.G.; Nascimento, K. S.; Isídro, Renato; Delatorre, Plínio; Rocha, Bruno Anderson Matias daVatairea guianensis seeds, a typical plant from the Brazilian Amazon region that belongs to the Dalbergieae tribe, possess a lectin that was isolated by precipitation with solid ammonium sulfate followed by guar gum affinity chromatography. This lectin was named VGL. The V. guianensis lectin strongly agglutinated rabbit erythrocytes and was inhibited by d-galactose and d-galactose-derived sugars, especially N-a...
A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partia...
Alves, Ana Cecília; Vasconcelos, Mayron Alves de; Santiago, Mayara Quiroz; Pinto-Junior, Vanir Reis; Osterne, Vinicius José SilvaA novel lectin from seeds of Clathrotropis nitida (CNA) was purified and characterized. CNA is a glycoprotein containing approximately 3.3% carbohydrates in its structure. CNA promoted intense agglutination of rabbit erythrocytes, which was inhibited by galactosides and porcine stomach mucin (PSM). The lectin maintained its hemagglutinating activity after incubation in a wide range of temperatures (30-60 °C) an...
Contribution of the carbohydrate-binding ability of Vatairea guianensis lectin ...
Marques, Gabriela Fernandes Oliveira; Osterne, Vinicius José Silva; Almeida, Livia M.; Oliveira, Messias Vital; Brizeno, Luiz André CavalcanteVatairea guianensis lectin (VGL), Dalbergiae tribe, is a N-acetyl-galactosamine (GalNAc)/Galactose (Gal) lectin previously purified and characterized. In this work, we report its structural features, obtained from bioinformatics tools, and its inflammatory effect, obtained from a rat paw edema model. The VGL model was obtained by homology with the lectin of Vatairea macrocarpa (VML) as template, and we used it ...
Structural basis for both pro- and anti-inflammatory response induced by mannos...
Rocha, Bruno Anderson Matias da; Delatorre, Plínio; Oliveira, Taianá Maia; Benevides, R. G.; Pires, Alana de Freitas; Sousa, Albertina A.S.Legume lectins, despite high sequence homology, express diverse biological activities that vary in potency and efficacy. In studies reported here, the mannose-specific lectin from Cymbosema roseum (CRLI), which binds N-glycoproteins, shows both pro-inflammatory effects when administered by local injection and anti-inflammatory effects when by systemic injection. Protein sequencing was obtained by Tandem Mass Sp...
Venom of the giant ant Dinoponera quadriceps attenuates inflammatory pain in mo...
Assreuy, Ana Maria Sampaio; Adjafre, Beatriz Lima; Sousa, Évilla Rayanne Oliveira; Alves, Ana Paula Negreiros Nunes; Pires, Alana de FreitasArthropod venoms are potential sources of bioactive substances, providing tools for the validation of popular use and new drugs design. Ants belonging to the genus Dinoponera are used in the folk medicine to treat inflammatory conditions. It was previously demonstrated that the venom of the giant ant Dinoponera quadriceps (DqV), containing a mixture of polypeptides, elicit antinociceptive effect in mice models ...
The effect of Cratylia floribunda lectin on renal hemodynamics and ion transport
Havt, Alexandre; Assreuy, Ana Maria Sampaio; Nascimento, Nilberto Robson Falcão do; Fonteles, Manassés Claudino; Pereira, Lívia de PaulaLectins have been described as glycoproteins that reversibly and specifically bind to carbohydrates. Legume lectins isolated from the subtribe Diocleinae (Canavalia, Dioclea and Cratylia) are structurally homologous with respect to their primary structures. The Diocleinae lectins of Canavalia brasiliensis, Dioclea guianensis and Canavalia ensiformis have been shown to distinctly alter physiological parameters i...
Polysaccharide extract of Mimosa tenuiflora stem barks stimulates acute inflamm...
Silva-Leite, Kaira Emanuella Sales da; Queiroz, Cinthia Silva; Madeira, Juliana da Costa; Soares, Pedro Marcos Gomes; Pereira, Maria GonçalvesMimosa tenuiflora (Mimosaceae) or “jurema-preta” is well distributed in the northeast Brazil, being popularly used to treat skin lesions, burns and inflammation. The healing effect of the alcoholic extract prepared with its barks corroborates the popular use. This study aimed to evaluate the inflammatory response of polysaccharides extracted from M. tenuiflora barks (EP-Mt) by methanol/NaOH and ethanol precipit...
Protective effects of ethanolic extract from the red algae Amansia multifida on...
Aragão, Gislei Frota; Nonato, Dayanne Terra Tenório; Ponte, Edson Lopes da; Sales, Jonathan Rodrigues; Alencar, Daniel Barroso; Sampaio, Silvana SakerThis study aimed to investigate the EEAm effect in mice models of nociception, inflammation and in behavioral tests evaluating the central nervous system. EEAm had inhibitory effects in the following tests: acetic acid-induced writhing (78%); formalin (62% - inflammatory phase); open field (46%). EEAm increased the nociceptive latency (56%) in tail flick test and increased the death-latency by 36% in the pentyl...
The effect of Cratylia floribunda lectin on renal hemodynamics and ion transport
Havt, Alexandre; Assreuy, Ana Maria Sampaio; Nascimento, Nilberto Robson Falcão do; Fonteles, Manassés Claudino; Pereira, Lívia de PaulaAs lectinas são descritas como (glico)proteínas que se ligam, especificamente e reversivelmente, a carboidratos. Lectinas de leguminosas isoladas da subtribo Diocleinae (Canavalia, Dioclea eCratylia) são estruturalmente homólogas em relação às suas estruturas primárias. Demonstrou-se que as lectinas de DiocleinaeCanavalia brasiliensis, Dioclea guianensis eCanavalia ensiformis alteram diferentemente parâmetros f...