10 documents found, page 1 of 1
Peach palm core collection in Brazilian Amazonia
Cristo-Araújo, Michelly de; Doriane, Picanço Rodrigues; Astolfi-Filho, Spártaco A.T.; Clement, Charles RolandThe Peach palm Active Germplasm Bank has abundant genetic diversity in its holdings. Because it is a live collection, maintenance, characterization and evaluation are expensive, restricting its use. One way to promote more efficient use is to create a Core Collection, a set of accessions with at least 70% of the genetic diversity of the full collection with minimal repetition. The available geographic, molecula...
Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (...
Chagas, Andrezza C.; Medeiros, Jansen Fernandes de; Astolfi-Filho, Spártaco A.T.; Py-Daniel, VictorIn this study, anticoagulant activity was detected in salivary gland homogenates (SGHs) of Thyrsopelma guianense (Diptera: Simuliidae). The SGH yielded 1.07 μg ± 0.03 (n = 15) of total soluble protein per pair of glands. In addition, following SDS-PAGE (12.5% gel) and silver nitrate staining, 12 polypeptides with molecular weights ranging from 14-69 kDa were detected in all physiological ages analyzed (12 h, 24...
Analysis of the genetic variability of peach palm (Bactris gasipaes) in the Yur...
Silva, Cirlande Cabral da; Doriane, Picanço Rodrigues; Clement, Charles Roland; Astolfi-Filho, Spártaco A.T.Molecular markers were used to verify the existence of one or more primitive peach palm landraces, including Pampa Hermosa, in the microregion near Yurimáguas, Peru. This region provides the highest amount of seeds for peach palm heart agribusinesses in Brazil. In this analysis, we used 120 peach palm plants (Bactris gasipaes var. gasipaes) from four river basins around Yurimáguas. We used six primers, generati...
Use of AFLPs to distinguish landraces of pejibaye (Bactris gasipaes) in Brazili...
Clement, Charles Roland; Sousa, Nelcimar Reis; Doriane, Picanço Rodrigues; Astolfi-Filho, Spártaco A.T.; Moreno, Yolanda Núñez; Pascual, Vicente TorresAlthough the first inhabitants of western Amazonia domesticated pejibaye (Bactris gasipaes Kunth, Palmae) or peach palm for its fruits, today it is widely planted for its heart-of-palm. Like other domesticates, pejibaye presents a complex hierarchy of landraces developed before the conquest of the Americas. The existence of three landraces (Pará, Solimões, Putumayo) was proposed along the Amazonas and Solimões ...
Molecular-genetic analysis for validation of peach palm (Bactris gasipaes Kunt)...
Silva, Cirlande Cabral da; Doriane, Picanço Rodrigues; Clement, Charles Roland; Astolfi-Filho, Spártaco A.T.Numerous landraces of peach palm (Bactris gasipaes var. gasipaes) have been described in the Neotropics and are conserved in a genebank of INPA. We used RAPD markers to analyze the genetic diversity of eight peach palm landraces and two wild populations. Eight primers generated 124 markers; 101 markers were polymorphic (81.5 %). Observed heterozygosity was 0.38 and polymorphism was 93 %, both slightly higher th...
The Genome of Anopheles darlingi, the main neotropical malaria vector
Marinotti, Osvaldo; Cerqueira, Gustavo C.; Almeida, Luiz G.P.; Ferro, Maria Inês Tiraboschi; Loreto, Elgion Lúcio da Silva; Zaha, ArnaldoAnopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and f...
Conservation implications of the mating system of the Pampa Hermosa landrace of...
Picanço-Rodrigues, Doriane; Astolfi-Filho, Spártaco A.T.; Lemes, Maristerra R.; Gribel, Rogério; Magno Sebbenn, Alexandre; Clement, Charles RolandPeach palm (Bactris gasipaes) is cultivated by many indigenous and traditional communities from Amazonia to Central America for its edible fruits, and is currently important for its heart-of-palm. The objective of this study was to investigate the mating system of peach palm, as this is important for conservation and breeding. Eight microsatellite loci were used to genotype 24 open-pollinated progenies from thr...
Method for obtaining high-resolution proteomic analysis from pericarps of guarana
Souza, Andréa L.; Angelo, P.; Nogueira, P. P O; Gonçalves, José Francisco Carvalho de; Franco, A. M.; Astolfi-Filho, Spártaco A.T.Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract pre...
The complete genome sequence of Chromobacterium violaceum reveals remarkable an...
Vasconcelos, Ana Tereza; De-Almeida, Darcy Fontoura; Hungría, Mariangela; Guimarães, Cláudia Teixeira; Antônio, Regina VasconcellosChromobacterium violaceum is one of millions of species of free-living microorganisms that populate the soil and water in the extant areas of tropical biodiversity around the world. Its complete genome sequence reveals (i) extensive alternative pathways for energy generation, (ii) ≈500 ORFs for transport-related proteins, (iii) complex and extensive systems for stress adaptation and motility, and (iv) wide-spre...
Electrophoresis and spectrometric analyses of adaptation-related proteins in th...
Cordeiro, Isabelle Bezerra; Castro, Diogo Pereira de; Nogueira, P. P O; Angelo, P.; Nogueira, Paulo Afonso; Gonçalves, José Francisco Carvalho deChromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected was 1985; the number ranged from 99 to 380 in each assay....