8 documents found, page 1 of 1
Proteogenomic analysis of Georgfuchsia toluolica revealed unexpected concurrent...
Atashgahi, Siavash; Oosterkamp, Margreet J.; Peng, Peng; Frank, Jeroen; Kraft, Beate; Hornung, Bastian; Schleheck, David; Lücker, SebastianDenitrifying Betaproteobacteria play a key role in the anaerobic degradation of monoaromatic hydrocarbons. We performed a multi-omics study to better understand the metabolism of the representative organism Georgfuchsia toluolica strain G5G6 known as a strict anaerobe coupling toluene oxidation with dissimilatory nitrate and Fe(III) reduction. Despite the genomic potential for degradation of different carbon so...
Long-chain fatty acids degradation by desulfomonile species and proposal of can...
Alves, J. I.; Salvador, Andreia Filipa Ferreira; Carvalho, Ana Rita Castro; Zheng, Ying; Nijsse, Bart; Atashgahi, Siavash; Sousa, Diana Zita MachadoMicrobial communities with the ability to convert long-chain fatty acids (LCFA) coupled to sulfate reduction can be important in the removal of these compounds from wastewater. In this work, an enrichment culture, able to oxidize the long-chain fatty acid palmitate (C16:0) coupled to sulfate reduction, was obtained from anaerobic granular sludge. Microscopic analysis of this culture, designated HP culture, reve...
Organohalide-respiring Desulfoluna species isolated from marine environments
Peng, Peng; Goris, Tobias; Lu, Yue; Nijsse, Bart; Burrichter, Anna; Schleheck, David; Koehorst, Jasper J.; Liu, Jie; Sipkema, DetmerThe genus Desulfoluna comprises two anaerobic sulfate-reducing strains, D. spongiiphila AA1T and D. butyratoxydans MSL71T, of which only the former was shown to perform organohalide respiration (OHR). Here we isolated a third strain, designated D. spongiiphila strain DBB, from marine intertidal sediment using 1,4-dibromobenzene and sulfate as the electron acceptors and lactate as the electron donor. Each strain...
Microbial synthesis and transformation of inorganic and organic chlorine compounds
Atashgahi, Siavash; Liebensteiner, Martin G.; Janssen, Dick B.; Smidt, Hauke; Stams, Alfons Johannes Maria; Sipkema, DetmerOrganic and inorganic chlorine compounds are formed by a broad range of natural geochemical, photochemical and biological processes. In addition, chlorine compounds are produced in large quantities for industrial, agricultural and pharmaceutical purposes, which has led to widespread environmental pollution. Abiotic transformations and microbial metabolism of inorganic and organic chlorine compounds combined wit...
A benzene-degrading nitrate-reducing microbial consortium displays aerobic and ...
Atashgahi, Siavash; Hornung, Bastian; van der Waals, Marcelle J.; da Rocha, Ulisses Nunes; Hugenholtz, Floor; Nijsse, Bart; Molenaar, DouweIn this study, we report transcription of genes involved in aerobic and anaerobic benzene degradation pathways in a benzene-degrading denitrifying continuous culture. Transcripts associated with the family Peptococcaceae dominated all samples (2136% relative abundance) indicating their key role in the community. We found a highly transcribed gene cluster encoding a presumed anaerobic benzene carboxylase (AbcA a...
Prospects for harnessing biocide resistance for bioremediation and detoxification
Atashgahi, Siavash; Sánchez-Andrea, Irene; Heipieper, Hermann J.; van der Meer, Jan R.; Stams, Alfons Johannes Maria; Smidt, HaukeProkaryotes in natural environments respond rapidly to high concentrations of chemicals and physical stresses. Exposure to anthropogenic toxic substancessuch as oil, chlorinated solvents, or antibioticsfavors the evolution of resistant phenotypes, some of which can use contaminants as an exclusive carbon source or as electron donors and acceptors. Microorganisms similarly adapt to extreme pH, metal, or osmotic ...
Geochemical and microbial community determinants of reductive dechlorination at...
Atashgahi, Siavash; Lu, Yue; Zheng, Ying; Saccenti, Edoardo; Suarez-Diez, Maria; Ramiro-Garcia, Javier; Eisenmann, Heinrich; Elsner, MartinBiostimulation is widely used to enhance reductive dechlorination of chlorinated ethenes in contaminated aquifers. However, the knowledge on corresponding biogeochemical responses is limited. In this study glycerol was injected in an aquifer contaminated with cis-dichloroethene (cDCE), and geochemical and microbial shifts were followed for 265 days. Consistent with anoxic conditions and sulfate reduction after ...
Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus d...
Oosterkamp, Margreet J.; Boeren, Sjef; Atashgahi, Siavash; Plugge, Caroline M.; Schaap, Peter J.; Stams, Alfons Johannes MariaAlicycliphilus denitrificans strain BC grows anaerobically on acetone with nitrate as electron acceptor. Comparative proteomics of cultures of A. denitrificans strain BC grown on either acetone or acetate with nitrate was performed to study the enzymes involved in the acetone degradation pathway. In the proposed acetone degradation pathway, an acetone carboxylase converts acetone to acetoacetate, an AMP-depende...