630 documents found, page 1 of 63
Diversity and comparative genomic analysis of Pseudomonas syringae pv. actinidi...
Gimranov, Emil; Santos, Marino; Monteiro, Rodrigo; Azeredo, Joana; Santos, Conceição; Moura, LuísaPseudomonas syringae pv. actinidiae is a pandemic agent of bacterial canker in kiwifruit. The virulence of bacteria can be mediated by horizontal gene transfer in a co-evolutionary process with other microorganisms, enriching their genetic repertoire and enhancing adaptation strategies and fitness. Temperate bacteriophages can integrate into bacterial genomes, increasing genetic diversity and potentially improv...
Treatment of patients at high risk of osteoporotic fracture in a USF: the impor...
Sotana, Carolina; Ribeiro, Daniela; Azeredo, Joana; Peixoto, Margarida; Chagas, JoanaIntroduction: Osteoporosis is a bone disease whose prevalence has been increasing, particularly in elderly females. The main clinical consequence is the fragility fracture, leading to increased morbidity and mortality, and a decreased quality of life for the patient, representing a significant socioeconomic burden. The current challenge is the early identification of individuals at higher risk of fracture who r...
Combination of phages and antibiotics with enhanced killing efficacy against du...
Akturk, Ergun; Pinto, Graça; Ostyn, Lisa; Crabbé, Aurélie; Melo, Luís Daniel Rodrigues; Azeredo, Joana; Coenye, TomPseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens commonly found in biofilm-associated polymicrobial respiratory infections that are challenging to control. Studies performed in laboratory standard conditions suggest that bacterio(phages) and antibiotic combinations are more active against bacterial communities and biofilms than each agent alone. The purpose of this work was to study ...
Successful bacteriophage therapy of pyoderma caused by methicillin-resistant St...
Mota-Sá, Cátia; Rodrigues, Cláudia; Silva, Vanessa; Azeredo, Joana; Melo, Luís Daniel RodriguesPyoderma caused by methicillin-resistant Staphylococcus pseudintermedius poses significant challenges to canine health. Successful therapy requires an integrated approach to prudent antimicrobial use. This report describes the successful treatment of a dog with generalised superficial pyoderma using a topical formulation of S. pseudintermedius phage applied once daily for a period of 30 days.; A piodermite caus...
Removal of virus from hands: a study on the role of hand wash and drying
Pinto, Graça; Rodrigues, Diana Alexandra Ferreira; Macieira, Mariana; Gião, Maria Salomé; Reis, Cassilda M.; Woolhouse, Sarah; Azeredo, JoanaSummary Background Proper hand hygiene is highly important to control pathogens transmission. Although the effect of washing and drying on hands bacterial contamination has been reported, studies on viruses are scarce. Aim To assess the viral load of artificially contaminated hands after washing and after drying. Methods 30 volunteers filled a questionnaire about hand hygiene and participated in microbial assay...
European general practitioners' attitudes towards person-centred care and facto...
Petriček, Goranka; Klemenc-Ketiš, Zalika; Ožvačić Adžić, Zlata; Cerovečki, Venija; Willems, Sara; Tsimtsiou, Zoi; Zelko, Erika; Collins, ClaireBACKGROUND: Person-centred care (PCC) is a fundamental principle in general practice, emphasising practices tailored to individual patient preferences, needs, and values. Despite the importance of PCC, general practitioners (GPs) face obstacles in effectively implementing it, with associated factors remaining unclear. OBJECTIVES: The PACE GP/FP study aims to explore GPs' attitudes towards PCC and the factors fa...
CRISPR‑Cas9 engineered Saccharomyces cerevisiae for endolysin delivery to comba...
Sáez Moreno, David; Cunha, Joana; Melo, Luís Daniel Rodrigues; Tanaka, Kenya; Bamba, Takahiro; Hasunuma, Tomosiha; Azeredo, Joana; Domingues, LucíliaListeriosis is an infection caused by the consumption of food contaminated with Listeria monocytogenes. It leads to febrile gastroenteritis, central nervous system infections, and even death in risk populations. Bacteriophage endolysins selectively kill bacteria hydrolyzing their cell walls and have emerged as a potential tool for listeriosis control. Ply511 is an anti-Listeria endolysin that has activity again...
Coagulase-Negative Staphylococci phages panorama: genomic diversity and in vitr...
Lopes, Maria Beatriz Afonso Sequeira; Silva, Maria Daniela Ferreira; Azeredo, Joana; Melo, Luís Daniel RodriguesCoagulase-negative staphylococci (CoNS) are commensal bacteria of the human skin and mucosal membranes. The incidence of nosocomial infections caused by these species is on the rise, leading to a potential increase in antibiotic tolerance and resistance. Phages are emerging as a promising alternative to combat CoNS infections. Scientists are isolating phages infecting CoNS with a particular interest in S. epide...
Outer membrane vesicles of carbapenem-resistant clinical Acinetobacter baumanni...
Monteiro, Rodrigo; Alcantud, Beatriz Santamarina; Piersma, Sjouke; Hendrickx, Antoni P. A.; Maaß, Sandra; Becher, Dörte; Azeredo, Joana; Bathoorn, ErikInfections caused by carbapenem-resistant Acinetobacter baumannii (A. baumannii; CRAb) are associated with high patient morbidity and mortality. The serious threat for human health imposed by CRAb was recently underscored by identification of close-to-untouchable carbapenem- and tetracycline-resistant isolates. Since outer membrane vesicles (OMVs) of Gram-negative bacteria may contribute to antimicrobial resist...
Lytic properties and genomic analysis of bacteriophage Brt_Psa3, targeting Pseu...
Gimranov, Emil; Oliveira, Hugo Alexandre Mendes; Santos, Conceição; Moura, Luísa; Azeredo, JoanaPseudomonas syringae pv. actinidiae (Psa) is the causative agent of bacterial canker in kiwifruit (Actinidia spp.). Psa biovar 3 is the most prevalent and virulent, causing frequent and severe outbreaks worldwide. While current treatments have low efficacy, bacteriophages emerge as possible environmentally safe alternative biocontrol agents. In this study, bacteriophage Brt\_Psa3 was isolated from the soil of a...