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An in vitro model of catheter-associated urinary tract infections to investigat...

Azevedo, A. S.; Almeida, Carina; Gomes, Luciana C.; Ferreira, Carla; Mergulhão, Filipe J.; Melo, Luís F.; Azevedo, Nuno F.

Uncommon bacteria, such as Delftia tusurhatensis have been isolated from CAUTIs in combination with well-established pathogenic bacteria such as Escherichia coli. Nonetheless, the reason why E. coli coexists with other bacteria instead of outcompeting and completely eliminating them is unknown. As such, a flow cell reactor simulating the hydrodynamic conditions found in CAUTIs (shear rate of 15 s1) was used to ...


Detection and discrimination of microorganisms using Locked Nucleic Acid - Fluo...

Azevedo, A. S.; Pereira, B.; Almeida, Carina; Melo, Luís F.; Wengel, J.; Azevedo, N. F.

The impact of multispecies biofilms on catheter-associated urinary tract infections outcome is still unclear due to the lack of adequate methodologies to discriminate the populations in situ. Employing fluorescence in situ hybridization (FISH) to discriminate the populations in a biofilm, can contribute to the understanding of microorganisms interactions in this structures. Consequently, this information might ...


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