53 documents found, page 1 of 6
Phage display identified peptide with selectivity for human osteoarthritic chon...
Martins, Ivone M.; Canadas, Raphaël F.; Pereira, Hélder; Azeredo, Joana; Reis, R. L.; Oliveira, J. M.; Azevedo, Helena S.Osteoarthritis (OA) is one of the most common joint disorders in western populations, affecting millions of people worldwide and with a rising incidence as life expectancy continues to increase. Current therapies for OA management fail to halt the progressive degradation of articular cartilage, urging the need for more effective therapies to improve cartilage function and enhance patient's quality of life. Thro...
Soft Matter for Biomedical Applications
Azevedo, Helena S.; Mano, João F.; Borges, JoãoDynamic soft materials that have the ability to expand and contract, change stiffness, self-heal or dissolve in response to environmental changes, are of great interest in applications ranging from biosensing and drug delivery to soft robotics and tissue engineering. This book covers the state-of-the-art and current trends in the very active and exciting field of bioinspired soft matter, its fundamentals and co...
Supramolecular presentation of hyaluronan onto model surfaces for studying the ...
Xinqing Pang; O'Malley, Clare; Borges, João; Rahman, Muhammad M.; Collis, Dominic W. P.; Mano, João F.; Mackenzie, Ian C.; Azevedo, Helena S.The supramolecular presentation of extracellular matrix components on surfaces provides a platform for the investigation and control of cell behavior. Hyaluronan (HA) is one of the main components of the extracellular environment and has been shown to play an important role in different cancers and their progression. However, current methods of HA immobilization often require its chemical modification. Herein, ...
Nanostructured interfacial self-assembled peptide–polymer membranes for enhance...
Ribeiro, Sofia; Radvar, Elham; Yejiao Shi; Borges, J.; Pirraco, Rogério P.; Leonor, I. B.; Mano, J. F.; Reis, R. L.; Mata, A.; Azevedo, Helena S.Soft interfacial materials, such as self-assembled polymer membranes, are gaining increasing interest as biomaterials since they can provide selective barriers and/or controlled affinity interactions important to regulate cellular processes. Herein, we report the design and fabrication of multiscale structured membranes integrating selective molecular functionalities for potential applications in bone regenerat...
In vitro chondrogenic commitment of human Wharton's jelly stem cells by co-cult...
Pereira, R. C.; Costa-Pinto, A. R.; Frias, A. M.; Neves, N. M.; Azevedo, Helena S.; Reis, R. L.Wharton's jelly stem cells (WJSCs) are a potential source of transplantable stem cells in cartilage-regenerative strategies, due to their highly proliferative and multilineage differentiation capacity. We hypothesized that a non-direct co-culture system with human articular chondrocytes (hACs) could enhance the potential chondrogenic phenotype of hWJSCs during the expansion phase compared to those expanded in m...
Nanostructured interfacial self-assembled peptide–polymer membranes for enhance...
Ribeiro, Sofia; Radvar, Elham; Shi, Yejiao; Borges, João; Pirraco, Rogério P.; Leonor, Isabel B.; Mano, João F.; Reis, Rui L.; Mata, ÁlvaroSoft interfacial materials, such as self-assembled polymer membranes, are gaining increasing interest as biomaterials since they can provide selective barriers and/or controlled affinity interactions important to regulate cellular processes. Herein, we report the design and fabrication of multiscale structured membranes integrating selective molecular functionalities for potential applications in bone regenerat...
Phage display technology in biomaterials engineering: progress and opportunitie...
Martins, Ivone M.; Reis, R. L.; Azevedo, Helena S.The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acschembio.5b00717. Table S1 shows the follow-up analysis on the subsequent applications of peptide sequences listed in Table 1 (PDF).; The field of regenerative medicine has been gaining momentum steadily over the past few years. The emphasis in regenerative medicine is to use various in-vitro and in-vivo app...
Identification of peptides targeting human osteoarthritic chondrocytes using ph...
Martins, Ivone M.; Nóbrega, Franklin Luzia; Pereira, Hélder; Azeredo, Joana; Kluskens, Leon; Reis, R. L.; Azevedo, Helena S.Osteoarthritis (OA) is one of the most common degenerative joint disease and is characterized by a progressive degradation of articular cartilage extracellular matrix (ECM) leading to loss of joint mobility and function, accompanied by chronic pain. Currently used therapies for cartilage repair are still far from generating regenerated tissue with quality and stability comparable to native cartilage. We hypothe...
Co-assembly, spatiotemporal control and morphogenesis of a hybrid protein-pepti...
Inostroza-Brito, K. E.; Collin, E.; Siton-Mendelson, O.; Smith, K. H.; Monge-Marcet, A.; Ferreira, D. S.; Rodríguez, R. P.; Alonso, M.Controlling molecular interactions between bioinspired molecules can enable the development of new materials with higher complexity and innovative properties. Here we report on a dynamic system that emerges from the conformational modification of an elastin-like protein by peptide amphiphiles and with the capacity to access, and be maintained in, non-equilibrium for substantial periods of time. The system enabl...
Molecularly engineered self-assembling membranes for cell-mediated degradation
Ferreira , Daniela S.; Lin, Yi-An; Cui, Honggang; Hubbell, Jeffrey A.; Reis, R. L.; Azevedo, Helena S.The use of peptide engineering to develop self-assembling membranes that are responsive to cellular enzyme activities is reported. The membranes are obtained by combining hyaluronan (HA) and a rationally designed peptide amphiphile (PA) containing a proteolytic domain (GPQGIWGQ octapeptide) sensitive to matrix metalloproteinase-1 (MMP-1). Insertion of an octapeptide in a typical PA structure does not disturb it...