8 documents found, page 1 of 1
Neuron–microglia contact-dependent mechanisms attenuate methamphetamine-induced...
Bravo, Joana; Ribeiro, Inês; Terceiro, Ana Filipa; Andrade, Elva B.; Portugal, Camila Cabral; Lopes, Igor M.; Azevedo, Maria M.; Sousa, MafaldaExposure to methamphetamine (Meth) has been classically associated with damage to neuronal terminals. However, it is now becoming clear that addiction may also result from the interplay between glial cells and neurons. Recently, we demonstrated that binge Meth administration promotes microgliosis and microglia pro-inflammation via astrocytic glutamate release in a TNF/IP3R2-Ca2+-dependent manner. Here, we inves...
Neuron-microglia contact-dependent mechanisms attenuate methamphetamine-induced...
Bravo, Joana; Ribeiro, Inês Moreira; Terceiro, Ana Filipa; Andrade, Elva B.; Portugal, Camila Cabral; Lopes, Igor M.; Azevedo, Maria M.; Sousa, MafaldaExposure to methamphetamine (Meth) has been classically associated with damage to neuronal terminals. However, it is now becoming clear that addiction may also result from the interplay between glial cells and neurons. Recently, we demonstrated that binge Meth administration promotes microgliosis and microglia pro-inflammation via astrocytic glutamate release in a TNF/IP<sub>3</sub>R2-Ca<sup>2+</sup>-dependent ...
Immunophenotype of gastric tumors unveils a pleiotropic role of regulatory T ce...
Rocha, Sara; Basto, Afonso; Ijsselsteijn, Marieke E.; Teles, Sara P.; Azevedo, Maria M.; Gonçalves, Gilza; Gullo, Irene; Almeida, Gabriela M.Gastric cancer (GC) patients display increased regulatory T cell (Tregs) numbers in peripheral blood and among tumor-infiltrating lymphocytes. Nevertheless, the role of Tregs in GC progression remains controversial. Here, we sought to explore the impact of Tregs in GCs with distinct histology, and whether Tregs can directly influence tumor cell behavior and GC development. We performed a comprehensive immunophe...
Involvement of calpains in adult neurogenesis: implications for stroke
Machado, Vanessa M.; Morte, Maria I.; Carreira, Bruno P.; Azevedo, Maria M.; Takano, Jiro; Iwata, Nobuhisa; Saido, Takaomi C.; Asmussen, HanneloreCalpains are ubiquitous proteases involved in cell proliferation, adhesion and motility. In the brain, calpains have been associated with neuronal damage in both acute and neurodegenerative disorders, but their physiological function in the nervous system remains elusive. During brain ischemia, there is a large increase in the levels of intracellular calcium, leading to the activation of calpains. Inhibition of...
Proteolytic systems and AMP-activated protein kinase are critical targets of ac...
Fernandes, Ângela Margarida Alves; Azevedo, Maria M.; Pereira, Olga; Marques, Belém Sampaio; Paiva, Artur; Neves, Margarida Correia; Castro, IsabelThe therapeutic strategies against acute myeloid leukemia (AML) have hardly been modified over four decades. Although resulting in a favorable outcome in young patients, older individuals, the most affected population, do not respond adequately to therapy. Intriguingly, the mechanisms responsible for AML cells chemoresistance/ susceptibility are still elusive. Mounting evidence has shed light on the relevance o...
Involvement of calpains in adult neurogenesis: implications for stroke
Machado, Vanessa M.; Morte, Maria I.; Carreira, Bruno P.; Azevedo, Maria M.; Takano, Jiro; Iwata, Nobuhisa; Saido, Takaomi C.; Asmussen, HanneloreCalpains are ubiquitous proteases involved in cell proliferation, adhesion and motility. In the brain, calpains have been associated with neuronal damage in both acute and neurodegenerative disorders, but their physiological function in the nervous system remains elusive. During brain ischemia, there is a large increase in the levels of intracellular calcium, leading to the activation of calpains. Inhibition of...
Determination of chitin content in fungal cell wall: an alternative flow cytome...
Costa-de-Oliveira, Sofia; Silva, Ana P.; Miranda, Isabel M.; Salvador, Alexandre; Azevedo, Maria M.; Munro, Carol A.; Rodrigues, Acácio G.The conventional methods used to evaluate chitin content in fungi, such as biochemical assessment of glucosamine release after acid hydrolysis or epifluorescence microscopy, are low throughput, laborious, time-consuming, and cannot evaluate a large number of cells. We developed a flow cytometric assay, efficient, and fast, based on Calcofluor White staining to measure chitin content in yeast cells. A staining i...
Fitoquímicos presentes em frutos silvestres: evidência científica para a sua ac...
Azevedo, Maria M.; Santos, Dario J. L.; Silva , Amélia M.Os frutos silvestres, nomeadamente a amora, o arando vermelho, a framboesa, o mirtilo e a baga de sabugueiro, são particularmente ricos em fitoquímicos (e.g., flavonóides, taninos, polifenóis e ácidos fenólicos) cuja acção biológica se relaciona com protecção antioxidante, cardiovascular, acção anti-envelhecimento e anti-cancerígena. A obtenção de extractos purificados, a sua caracterização química e a elucidaç...