131 documents found, page 1 of 14

Combining nucleic acid mimics and spectral imaging with fluorescence in situ hy...

[Excerpt] Nucleic acid mimics (NAMs)- based assays, such as locked nucleic acid/2′-O- methyl-RNA- fluorescence in vivo hybridization (LNA/2′OMe- FISH), have been developed for the identification and spatial location of Helicobacter pylori directly in the stomach. While H. pylori is considered the main gastric pathogen, there is a diverse range of stomach colonizers that may be associated with disease in the sto...

Minimum information guideline for spectrophotometric and fluorometric methods t...

Introduction: The lack of reproducibility of published experimental studies is one of the major issues facing science today, and the field of biofilm research is no exception. While many factors contribute to this phenomenon, selective or insufficient reporting of experimental details in the published literature is one of the more significant causes. Hypothesis and aims: One effective strategy to improve reprod...

Optimizing locked nucleic acid/2-O-methyl-RNA fluorescence in situ hybridizatio...

Despite the successful application of locked nucleic acid/2-O-methyl- RNA fluorescence in situ hybridization (LNA/2OMe-FISH) procedures for bacteria detection, there is a lack of knowledge on the properties that affect hybridization. Such information is crucial for the rational design of the protocols, especially in multiplex assays. Hence, this work aimed to evaluate the effect of 3 essential factors on the LN...

Critical review on biofilm methods

Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or have a negative impact particularly when formed in industrial settings or on medical devices. As such, research into the formation and elimination of biofilms is important for many disciplines. Several new methodologies have been r...

Morphological transition of Helicobacter pylori adapted to water

Aim: This study aims to investigate the morphological transition of Helicobacter pylori during adaptation to water. Materials \& methods: Different strains were adapted to water. Changes regarding cultivability and cellular morphology were recorded. Expression of 11 genes involved in H. pylori morphological changes was evaluated by real-time PCR. Results: H. pylori presented increased cultivability in water aft...

#126. Nova estratégia para detetar e localizar patogénicos periodontais: a técn...

[Excerto] Objetivos: A compreensão da dinâmica periodontal biofilme-hospedeiro, in situ, é crucial para melhorar o diagnóstico e definir tratamentos mais racionais e eficazes. Este trabalho tem como objetivo o desenvolvimento de sondas de ácido peptídico nucleico (PNA), um mímico do DNA, para a identificac¸ão e localizac¸ão de Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans)ePorphyromonas gingivalis...

FISHji: new ImageJ macros for the quantification of fluorescence in epifluoresc...

Fluorescence in situ hybridization (FISH) is based on the use of fluorescent staining dyes, however, the signal intensity of the images obtained by microscopy is seldom quantified with accuracy by the researcher. The development of innovative digital image processing programs and tools has been trying to overcome this problem, however, the determination of fluorescent intensity in microscopy images still has is...

Metodologias para a identificação de microrganismos patogénicos em amostras ali...

Enabling systematic, harmonised and large-scale biofilms data computation: the ...

Background and Objective: Biofilms are receiving increasing attention from the biomedical community. Biofilm-like growth within human body is considered one of the key microbial strategies to augment resistance and persistence during infectious processes. The Biofilms Experiment Workbench is a novel software workbench for the operation and analysis of Biofilms experimental data. The goal is to promote the inter...

Agent-based spatiotemporal simulation of biomolecular systems within the open s...

Agent-based modelling is being used to represent biological systems with increasing frequency and success. This paper presents the implementation of a new tool for biomolecular reaction modelling in the open source Multiagent Simulator of Neighborhoods framework. The rationale behind this new tool is the necessity to describe interactions at the molecular level to be able to grasp emergent and meaningful biolog...