6 documents found, page 1 of 1
Selection and Characterisation of Minor Histocompatibility Antigen-Specific Reg...
Carolina Paulino, Pacini; Azevedo, Rita I.; Ramalhete, Luís; Lainé, Hugo A.J.; Soares, Maria V.D.; Lacerda, João F.Graft-versus-host disease is mediated by donor-derived T cells reactive against the recipient's broadly expressed minor histocompatibility antigens (mHA). Regulatory T cells (Treg) have been explored as a therapeutic approach for chronic GVHD (cGVHD). The promising results from polyclonal Treg trials in this setting have led us to develop a Treg product specific for mismatched minor antigens between patient and...
Suppression by allogeneic-specific regulatory T cells is dependent on the degre...
Bianchi, Joana; Vieira, Ana I. S.; Ligeiro, Dário; Azevedo, Rita I.; Lacerda, JoãoRegulatory T cell (Treg) infusion for graft-versus-host disease treatment has been increasingly investigated. However, polyclonal Treg may suppress the desired graft-versus-leukemia effect. Although allogeneic-specific (allo-specific) Treg may provide a more-targeted graft-versus-host disease treatment, there is the need to develop easily translatable expansion protocols and to better characterize their specifi...
Mesenchymal stromal cells induce regulatory T cells via epigenetic conversion o...
Azevedo, Rita I.; Minskaia, Ekaterina; Fernandes-Platzgummer, Ana; Vieira, Ana I. S.; da Silva, Cláudia Lobato; Cabral, Joaquim M.S.; Lacerda, JoãoRegulatory T cells (Treg) play a critical role in immune tolerance. The scarcity of Treg therapy clinical trials in humans has been largely due to the difficulty in obtaining sufficient Treg numbers. We performed a preclinical investigation on the potential of mesenchymal stromal cells (MSCs) to expand Treg in vitro to support future clinical trials. Human peripheral blood mononuclear cells from healthy donors ...
Naive and stem cell memory T cell subset recovery reveals opposing reconstituti...
Soares, Maria Vieira; Azevedo, Rita I.; Ferreira, Ines; Bucar, Sara; Ribeiro, Ana C.; Vieira, Ana I. S.; Pereira, Paulo N G; Ribeiro, Ruy M.The success of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in the treatment of hematological malignancies remains hampered by life-threatening chronic graft vs. host disease (cGVHD). Although multifactorial in nature, cGVHD has been associated with imbalances between effector and regulatory T cells (Treg). To further elucidate this issue, we performed a prospective analysis of patients underg...
Molecular markers distinguishing T Cell subtypes with TSDR strand-bias methylation
Minskaia, Ekaterina; Saraiva, Bárbara C.; Soares, Maria Vieira; Azevedo, Rita I.; Ribeiro, Ruy M.; Kumar, Saumya; Vieira, Ana I. S.; Lacerda, JoãoHuman regulatory CD4+CD25+FOXP3+ T cells (Treg) play important roles in the maintenance of self-tolerance and immune homeostasis in various disease settings and are also involved in the suppression of effective immune responses. These cells are heterogeneous in phenotype and function, and the ability to reliably distinguish between various FOXP3-expressing subpopulations can affect the development of successful...
The role of IL-7 in the homeostasis of human naive and memory CD4+T cell subsets
Azevedo, Rita I.The main focus of this work is to study the homeostasis of human naive and memory CD4+ T cell subsets, particularly assessing the role of IL-7 in this process. For this purpose, we assessed the potentially distinct effects of IL-7 in the homeostasis of naive CD4+ T cell subsets defined by CD31 expression. We describe for the first time the preferential proliferation of the CD31+ subset within adult naive CD4+ T...