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Comprehensive DNA metabarcoding-based detection of non-indigenous invertebrates...

Lavrador, Ana S.; Amaral, Fábio G.; Moutinho, Jorge; Vieira, Pedro Emanuel Ferreira Reis; Costa, Filipe O.; Duarte, Sofia Alexandra Ferreira

eDNA metabarcoding has been increasingly employed in the monitoring of marine invertebrate non-indigenous species (NIS), in particular using filtered seawater. However, comprehensive detection of all NIS may require a diversity of sampling substrates. To assess the effectiveness of 5 sample types (hard and artificial substrates, water, zooplankton) on the recovery of invertebrates’ diversity, two marinas were m...


Surveillance of non-indigenous invertebrate species through DNA metabarcoding i...

Lavrador, Ana Sofia Castro; Amaral, Fábio G.; Vieira, Pedro Emanuel Ferreira Reis; Costa, Filipe O.; Duarte, Sofia Alexandra Ferreira

DNA metabarcoding has been widely used in biodiversity assessments as a complement to traditional morphology-based techniques. This technique is useful for the early detection and surveillance of non-indigenous species (NIS) in aquatic ecosystems. When introduced to new locations, NIS can establish, and become invasive, which may cause severe alterations to native ecosystems and biodiversity. As most introducti...


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