6 documents found, page 1 of 1
Determination of cell abundances and paralytic shellfish toxins in cultures of ...
B. Lopes, Marta; Amorim, Ana; Calado, Cecília; Reis Costa, PedroHarmful algal blooms are responsible worldwide for the contamination of fishery resources, with potential impacts on seafood safety and public health. Most coastal countries rely on an intense monitoring program for the surveillance of toxic algae occurrence and shellfish contamination. The present study investigates the use of near infrared (NIR) spectroscopy for the rapid in situ determination of cell concent...
Assessing plasmid bioprocess reproducibility and C‐source uptake stage through ...
B. Lopes, Marta; Calado, CecíliaBACKGROUND: A considerable effort has been put forth by the biopharmaceutical industry to guarantee high-quality products and patient safety. Ensuring the reproducibility of microbial cultures is essential to achieve such high standards. Reproducibility is usually assessed by offline, costly and time-consuming analyses of the final product, disregarding the culture behavior along time, which affects the final p...
Metabolic profiling of recombinant Escherichia coli cultivations based on high-t...
Sales, Kevin C.; Rosa, Filipa; Ribeiro Da Cunha, Bernardo; Sampaio, Pedro; B. Lopes, Marta; Calado, CecíliaEscherichia coli is one of the most used host microorganism for the production of recombinant products, such as heterologous proteins and plasmids. However, genetic, physiological and environmental factors influence the plasmid replication and cloned gene expression in a highly complex way. To control and optimize the recombinant expression system performance, it is very important to understand this complexity. ...
High-throughput FTIR-based bioprocess analysis of recombinant cyprosin production
Sampaio, Pedro; Sales, Kevin C.; Rosa, Filipa O.; B. Lopes, Marta; Calado, CecíliaTo increase the knowledge of the recombinant cyprosin production process in Saccharomyces cerevisiae cultures, it is relevant to implement efficient bioprocess monitoring techniques. The present work focuses on the implementation of a mid-infrared (MIR) spectroscopy-based tool for monitoring the recombinant culture in a rapid, economic, and high-throughput (using a microplate system) mode. Multivariate data ana...
Does nonlinear modeling play a role in plasmid bioprocess monitoring using four...
B. Lopes, Marta; Calado, Cecília; Figueiredo, Mario; Bioucas-Dias, JoseThe monitoring of biopharmaceutical products using Fourier transform infrared (FT-IR) spectroscopy relies on calibration techniques involving the acquisition of spectra of bioprocess samples along the process. The most commonly used method for that purpose is partial least squares (PLS) regression, under the assumption that a linear model is valid. Despite being successful in the presence of small nonlinearitie...
In Situ Near-Infrared (NIR) Versus High-Throughput Mid- Infrared (MIR) Spectros...
Sales, Kevin C.; Rosa, Filipa; Sampaio, Pedro N.; Fonseca, LuÍs P.; B. Lopes, Marta; Calado, CecíliaThe development of biopharmaceutical manufacturing processes presents critical constraints, with the major constraint being that living cells synthesize these molecules, presenting inherent behavior variability due to their high sensitivity to small fluctuations in the cultivation environment. To speed up the development process and to control this critical manufacturing step, it is relevant to develop high-thr...