4 documents found, page 1 of 1
NEDD8 conjugation in Schistosoma mansoni : genome analysis and expression profi...
Pereira, Roberta Verciano; Gomes, Matheus de Souza; Olmo, Roenick Proveti; Sousa, Daniel M.; Passos, Liana Konovaloff Jannotti; Babá, Élio HideoNEDD8 is an ubiquitin-like molecule that covalently binds to target proteins through an enzymatic cascade analogous to ubiquitylation. This modifier is known to bind to p53 and p73, as well as all Cullin family proteins, which are essential components of Skp1/Cul-1/F-box protein (SCF)-like Ub ligase complexes. Here, we focused on a genomic analysis of the genes involved in the NEDD8 conjugation pathway in Schis...
Preliminary analysis of miRNA pathway in Schistosoma mansoni.
Gomes, Matheus de Souza; Cabral, Fernanda Janku; Passos, Liana Konovaloff Jannotti; Carvalho, Omar; Rodrigues, Vanderlei; Babá, Élio HideoRNA silencing refers to a series of nuclear and cytoplasmatic processes involved in the post-transcriptional regulation of gene expression or post-transcriptional gene silencing (PTGS), either by sequence-specific mRNA degradation or by translational arrest. The best characterized small RNAs are microRNAs (miRNAs), which predominantly perform gene silencing through post-transcriptional mechanisms. In this work ...
A novel and efficient and low-cost methodology for purification of Macrotyloma ...
Santana, Marcos Aurélio de; Santos, Alexandre Martins Costa; Oliveira, Marcelo Eurípedes; Oliveira, Jamil Silvano de; Babá, Élio HideoThe N-acetyl-galactosamine specific lectin fromMacrotyloma axillare seeds (LMA)was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L−1 fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pHdependent assays showed best hemagglutinating activity at pH 6.0–8.0; being decreased at acidic/alkaline conditions...
Characterisation of major vault protein during the life cycle of the human para...
Reis, Eneida Virgínia; Pereira, Roberta Verciano; Gomes, Matheus de Souza; Passos, Liana Konovaloff Jannotti; Babá, Élio HideoVaults are ribonucleoproteins (13MDa) highly conserved among lower and higher eukaryotes. Their association produces a complex composed of three proteins named Major Vault Protein (MVP), vault (PolyADP-ribose) polymerase (VPARP) and Telomerase-associated protein (TEP1), plus a small untranslated RNA. The exact function of this complex is unknown, although the biological role of vaults has been associated with m...