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Padronização de multiplex PCR para detecção de dermatófitos em pelos e crostas ...
Leal,Carlos A.S.; Kim,Pomy C.P.; Almeida,Jonatas C.; Melo,Renata P.B.; Santos,André S.; Lima,Débora C.V.; Pinheiro Júnior,José W.; Mota,Rinaldo A.RESUMO: Objetivou-se padronizar uma reação do tipo multiplex PCR (mPCR) para detectar Microsporum canis, Microsporum gypseum e o complexo Trichophyton mentagrophytes em amostras de pelos e/ou crostas de cães e gatos. 250 amostras de pelos e/ou crostas de cães e gatos foram analisadas por meio de exame direto e cultura, o DNA das mesmas foi extraído para mPCR. Primers foram desenhados e como controle positivo da...
Recovery of Mollicutes from the reproductive tract of dairy cattle in the state...
Santos,Sandra B.; Pinheiro-Júnior,José W.; Mota,André R.; Santos,André S.; Alves,Bruno H.L.S.; Oliveira,Júnior M.B.; Silva,Leonildo B.G.Abstract: The aim of the present study was to report the occurrence of members of the Mollicutesclass in the reproductive system of dairy cattle in Brazil. Five farms containing dairy cattle were visited in January of 2012. In total, 100 cows of different ages, breeds and stages of lactation were examined in the present study. The cows were part of intensive or semi-intensive management systems and were submitt...
Occurrence of Toxoplasma gondii DNA in sheep naturally infected and slaughtered...
Bezerra,Mauro J.G.; Cruz,Jefferson A.L.O.; Kung,Eugênio S.; Silva,José G.; Santos,André S.; Moraes,Érica P.B.X.; Pinheiro Junior,José W.The aim of the present study was to assess the occurrence of antibodies to Toxoplasma gondii and to detect genomic DNA of the parasite in the reproductive organs, fetuses and fetal membranes of sheep in slaughterhouses in the state of Pernambuco, Brazil. The Indirect Immunofluorescence technique (IFA) was used for screening. The Polymerase Chain Reaction (PCR) was used to detect DNA of T. gondii in the animals ...
Molecular characteristics of Machado-Joseph disease mutation in 25 newly descri...
Lopes-Cendes,Iscia; Teive,Hélio G.A.; Cardoso,Francisco; Viana,Erika M.; Calcagnotto,Maria E.; Costa,Jaderson C. da; Trevisol-Bittencourt,Paulo C.Machado-Joseph disease (MJD) is a form of autosomal dominant spinocerebellar ataxia first described in North-American patients originating from the Portuguese islands of the Azores. Clinically this disorder is characterized by late onset progressive ataxia with associated features, such as: ophthalmoplegia, pyramidal and extrapyramidal signs and distal muscular atrophies. The causative mutation is an expansion ...
Frequency of the different mutations causing spinocerebellar ataxia (SCA1, SCA2...
Lopes-Cendesi,Iscia; Teive,Hélio G.A.; Calcagnotto,Maria E; da Costa,Jaderson C.; Cardoso,Francisco; Viana,Erika; Maciel,Jaime A.; Radvany,JoãoSpinocerebellar ataxia type 1 (SCA1), spinocerebellar ataxia type 2 (SCA2) and Machado-Joseph disease or spinocerebellar ataxia type 3 (MJD/SCA3) are three distinctive forms of autosomal dominant spinocerebellar ataxia (SCA) caused by expansions of an unstable CAG repeat localized in the coding region of the causative genes. Another related disease, dentatorubropallidoluysian atrophy (DRPLA) is also caused by a...