23 documents found, page 1 of 3
Effective separation of nucleic acids from complex bacterial lysates using thre...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniNo abstract available.
Purification of RNA from recombinant lysates using biocompatible amino-acid-bas...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniRNA-based biopharmaceuticals are being envisioned as a powerful tool for the development of innovative medicines for prevalent diseases. Despite their undeniable relevance, the ubiquitous unstable nature of RNA coupled with the laborious and costly methods required for its extraction and purification still challenge the widespread application of this biopolymer. On the basis of the high affinity between amino-a...
Aqueous biphasic systems composed by biocompatible amino acid-based ionic liqui...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniThe emergence of RNA as a promising biopharmaceutical paved the way for the development of innovative medicines with broad therapeutic and prophylactic efficiencies in infectious diseases and cancer. Notwithstanding their relevance on this field, the labile nature and intrinsic low stability of RNA coupled with the laborious and costly methods of extraction and purification of this biopolymer constitute the mai...
Optimization of the production and purification of non-viral vectors for gene t...
Valente, Ana I.; Castro, Leonor S.; Rios, Inês D.; Pedro, Augusto Q.; Tavares, Ana P. M.; Sousa, Fani; Freire, Mara G.Cancer ranks the second leading cause of death globally and has significant societal and economic costs. To address this issue, the production of biopharmaceuticals is standing out. Nucleic acids are gaining popularity in preventing infections and as therapeutic agents in gene therapy, aiming to fix the target dysfunction by transfecting eukaryotic cells with gene-based products. Non-viral vectors, including pl...
A tecnologia do ADN recombinante aplicada ao desenvolvimento de vacinas, com es...
Castro, Leonor S.; Freire, Mara G.; Pedro, Augusto Q.Surtos, epidemias e pandemias são responsáveis por grandes alterações económicas, políticas, e sociais, permitindo igualmente um elevado desenvolvimento científico e tecnológico. Ao longo da história, estes eventos têm-se vindo a intensificar devido ao aumento na mobilidade entre populações, o que se traduz num aumento das cadeias de transmissão de agentes infeciosos. Oficialmente declarada pandemia em 11/03/20...
Alternative protein purification technologies using immobilized ionic liquids a...
Castro, Leonor S.; Lobo, Guilherme S.; Neves, Márcia C.; Freire, Mara G.; Pedro, Augusto Q.The increase of life expectancy has been accompanied by an increment of chronic diseases, with biopharmaceuticals becoming one of the most effective clinical treatments for a broad range of diseases. However, their manufacturing can be time-consuming and expensive, thus limiting their utilization. Their manufacturing is typically divided in the upstream and downstream phases with the latter being responsible fo...
Purification of immunoglobulin Y from egg yolk using thermoresponsive aqueous m...
Vicente, Filipa A.; Castro, Leonor S.; Mondal, Dibyendu; Coutinho, João A. P.; Tavares, Ana P. M.; Ventura, Sónia P. M.; Freire, Mara G.Immunoglobulin Y (IgY) represents an important class of antibodies, being present in egg yolk, with relevant medical applications and involving non-invasive methods of extraction. However, due to the complexity of egg yolk, the purification levels required for use in most medical applications demands the application of multi-step and cost-intensive techniques. Therefore, it is of upmost importance to develop a ...
Stabilization and purification of RNA using biocompatible ionic liquids
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniThe widespread use of RNA for the development of novel therapies has been hindered due to issues associated with its inherent chemical instability and fast degradation by ribonucleases1. In addition, the traditional extraction and purification techniques of this biomolecule are time-consuming and require highly toxic reagents, demanding for improved technologies able to provide RNA with high integrity, purity a...
Stabilization and purification of RNA using biobased ionic liquids
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniThe ubiquitous unstable nature of RNA has been preventing its widespread use in the development of novel therapies. Moreover, the traditional RNA extraction and purification techniques are laborious and require highly toxic reagents, demanding for improved technologies able to provide RNA with high purity and biological activity. To overcome the described bottlenecks, ionic-liquid-based aqueous biphasic systems...
Interferon-Based Biopharmaceuticals: Overview on the Production, Purification, ...
Castro, Leonor S.; Lobo, Guilherme S.; Pereira, Patrícia; Freire, Mara G.; Neves, Márcia C.; Pedro, Augusto Q.The advent of biopharmaceuticals in modern medicine brought enormous benefits to the treatment of numerous human diseases and improved the well-being of many people worldwide. First introduced in the market in the early 1980s, the number of approved biopharmaceutical products has been steadily increasing, with therapeutic proteins, antibodies, and their derivatives accounting for most of the generated revenues....