8 documents found, page 1 of 1
Probe-based metagenomic pathogen detection: advancing laboratory capacity for c...
Ferreira, Rita; Coelho, Luís; Santos, João Dourado; Sobral, Daniel; Isidro, Joana; Mixão, Verónica; Pinto, Miguel; Nunes, Alexandra; Borrego, Maria JoséProbe-based pathogen enrichment, followed by NGS, is a promising tool for complex diagnosis, overcoming traditional challenges of shotgun metagenomics, namely small microbial/human genetic material ratio and demanding computational resources. Here, we assessed the combined detection performance of two Illumina probe-based panels, the Respiratory and the Urinary Pathogen ID panels (RPIP and UPIP), using 99 clini...
Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f ...
Gonzalez-Diaz, Aida; Carrera-Salinas, Anna; Pinto, Miguel; Cubero, Meritxell; van der Ende, Arie; Langereis, Jeroen D.; Domínguez, M. ÁngelesHaemophilus influenzae is an opportunistic pathogen adapted to the human respiratory tract. Non-typeable H. influenzae are highly heterogeneous, but few studies have analysed the genomic variability of capsulated strains. This study aims to examine the genetic diversity of 37 serotype f isolates from the Netherlands, Portugal, and Spain, and to compare all capsulated genomes available on public databases. Serot...
Antibody Binding and Complement-Mediated Killing of Invasive Haemophilus influe...
Dudukina, Elena; de Smit, Laura; Verhagen, Giel J.A.; van de Ende, Arie; Marimón, José María; Bajanca-Lavado, Paula; Ardanuy, Carmen; Marti, SaraHaemophilus influenzae is a Gram-negative bacterium that can be classified into typeable (types a through f) and nontypeable (NTHi) groups. This opportunistic pathogen asymptomatically colonizes the mucosal epithelium of the upper respiratory tract, from where it spreads to other neighboring regions, potentially leading to disease. Infection with NTHi can cause otitis media, sinusitis, conjunctivitis, exacerbat...
Identification of polysaccharide capsules among extensively drug-resistant geni...
González-Díaz, Aida; Tubau, Fe; Pinto, Miguel; Sierra, Yanik; Cubero, Meritxell; Càmara, Jordi; Ayats, Josefina; Bajanca-Lavado, Paula; Ardanuy, CarmenThe human commensal Haemophilus parainfluenzae is emerging as an opportunistic multidrug-resistant pathogen. The objectives of this work were to characterise a new capsular operon of extensively drug-resistant (XDR) H. parainfluenzae clinical isolates and study their resistance mechanisms using whole-genome sequencing. All strains were resistant to: ß-lactams, via amino acid changes in PBP3 (S385T, I442F, V511A...
Detection of the Hmw adhesins in clinical Haemophilus influenzae isolates from ...
Bosh, Ana; González, Aida; Carrera-Salinas, Anna; Cubero, Meritxell; Marimón, José María; Bajanca-Lavado, Paula; Ardanuy, Carmen; Marti, SaraBackground: Non-typeable Haemophilus influenzae (NTHi) forms part of the normal nasopharyngeal microbiota in humans, but it is also an opportunistic pathogen causing respiratory infections and bacteraemia. Recently, high molecular weight (HMW1) proteins have been identified as a key factor for cell invasion, a feature implicated in persistence during chronic infection(a). Our aims were to identify the different...
A osteomelite crónica pode ser o precedente de uma infeção por Corynebacterium ...
Faustino, Joana; Milheiro Silva, Tiago; Lameiras Campagnolo, João; Bajanca-Lavado, Paula; Gouveia, CatarinaIntrodução: A infeção por C. diphtheriae foi virtualmente eliminada de muitos países desenvolvidos, contudo é endémica na áfrica subsariana. A difteria cutânea é caracterizada por feridas crónicas não cicatrizantes, precedidas habitualmente por traumatismo. Caso Clínico: Rapariga de 11 anos, natural e residente na Guiné, internada na Guiné aos 10 anos por fratura exposta do úmero esquerdo secundária a queda da ...
Whole-genome-based characterization of invasive Haemophilus influenzae isolates...
Gonzalez Diaz, Aida; Pinto, Miguel; Bettencourt, Célia; Duarte, Silvia; Marti, Sara; Gomes, João Paulo; Bajanca-Lavado, PaulaIntroduction: Haemophilus influenzae (Hi) is responsible for severe invasive infections in both adults and children. Since the introduction in the year 2000 of the Hib vaccine, the incidence of disease has substantially declined, even though it doesn’t protect against non-typeable Hi (NTHi) isolates. Although not all NTHi are pathogenic, these are known to possess important virulence factors to promote coloniza...
Epidemiological analysis of invasive Haemophilus influenzae clinical isolates o...
Raeven, Elisabeth A.M.; González, Aida; van der Ende, Arie; Liñares, Josefina; Marimón, José María; Bajanca-Lavado, Paula; Langereis, Jeroen D.Background. Haemophilus influenzae is a human-restricted pathogen that forms part of the normal nasopharyngeal microbiota. The introduction of the H. influenzae serotype b vaccine has drastically decreased the number of bacteremia cases caused by H. influenzae serotype b (Hib). Conversely, the cases of non-typeable H. influenzae (NTHi) bacteremia have increased substantially. Therefore, we aimed to perform an e...